Fuzzy Systems

Swapnil G. Sanmukh ¹, Waman N. Paunikar ¹

Affiliations
1 Ecosystem Division, National Environmental Engineering Research Institute (NEERI), Nehru Marg, Nagpur-440020, Maharashtra, IN

Abstract

The Mycobacteriophages are the bacteriophages infecting different Mycobacterium species in nature. The hypothetical proteins in 17 different Mycobacteriophages were structurally and functionally characterized by using computational tools like CDD-Blast, Interproscan, pfam, COGS and protein structure prediction server (PS2 server). Near about 2188 hypothetical proteins were screened for presence of functional domains and structural classification. The function predictions for 586 hypothetical proteins and structure predictions for 171 hypothetical proteins were possible by using these Bioinformatics web tools. These results can prove helpful for proper understanding of functional, evolutionary and metabolic development of Mycobacterium species.

Keywords

Mycobacterium Species, Hypothetical Proteins, Bioinformatics Web Tools, Evolutionary and Metabolic Development.

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Swapnil G Sanmukh ¹, Waman N. Paunikar ², Dilip Budha Meshram ², Tarun K. Ghosh ²

Affiliations
1 Applied Aquatic Ecosystem Division, National Environmental Engineering Research Institute, Nehru Marg, Nagpur- 440020, IN
2 Applied Aquatic Ecosystem Division, National Environmental Engineering Research Institute, Nehru Marg, Nagpur-440020, IN

Abstract

The Halobacterium salinarum are extremely halophilic archaeon. The complete genome sequence of Halobacterium salinarum was deciphered, which revealed the presence of various gene for hypothetical proteins whose function are not yet understood. We analyzed complete genome of Halobacterium salinarum for hypothetical proteins from which 524 uncharacterized proteins showed conserved domains for biologically active components. Our attempt is to predict the function of these hypothetical proteins by the application of computational methods and Bioinformatics. The probable function prediction of the hypothetical protein was done by using Bioinformatics web tools like CDD-BLAST, INTERPROSCAN, PFAM and COGs by searching sequence databases for the presence of orthologous enzymatic conserved domains in the hypothetical sequences. These study revealed presences of enzymatic functional domain in Halobacterium salinarum; their roles are yet to be discovered. These deciphered enzymatic data for hypothetical proteins can be used for the understanding of functional, structural, evolutionary and metabolic development of Halobacterium spp. and its life cycle along with their role in host evolution and adaptation.

Keywords

Bioinformatics Web Tools, Conserved Domains, Uncharacterized Proteins, Life Cycle, Host Evolution.

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