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Rajnish, K. N.
- Impact of Hydrothermal Treatments on Elimination of Antinutrient Factors in Velvet Bean
Authors
1 Department of Biotechnology, Prathyusha Institute of Technology and Management, Aranvoyal Kuppam, Tiruvallur – 602 025, Tamil Nadu, IN
Source
Biometrics and Bioinformatics, Vol 4, No 3 (2012), Pagination: 105-108Abstract
Two germplasm seed materials, Mundanthurai (white coloured seed coat) and Myllaru (black coloured seed coat) of velvet bean (Mucuna prurieus var utilis) were investigated for antinutritional factors, total free phenols, tannins, and phyto haemagglutinating activity. Both the accessions contain relatively high levels of phenols and negligible tannin content. Without any specificity, the crude lectins (phytohaemagglutinnins) agglutinate the erythrocytes of human A, B, O blood groups. Between the hydrothermal treatments, cooking and autoclaving, cooking has completely eliminated lectin without any specificity in both the investigated germ plasm of velvet bean; whereas autoclaving has completely eliminated lectin activity without any specificity in Myllarus (white coloured) seed coat germplasm. However, it has eliminated lectin activity in Mundanthurai (white-coloured seed coat) with respect to erythrocytes from “B” and “O” blood groups. Both the treatments were not effective in complete elimination of total free phenols and tannins. In fact, tannins content has increased in Mundanthurai germ plasm (33 %) due to cooking and autoclaving (33 %).
Keywords
Velvet Bean, Antinutritional Factors, Free Phenols, Tannins, Phytohaemagglutinins, Germplasm, Cooking, Autoclaving.
- Effect of Certain Post Harvest Treatments on Elimination of Phytohaemagglutinins in Sword Bean (Canavalia gladiata (Jacq DC.))
Authors
1 Department of Biotechnology, Prathyusha Institute of Technology and Management, Aranvoyal Kuppam, Tiruvallur – 602 025, Tamil Nadu, IN
Source
Biometrics and Bioinformatics, Vol 4, No 3 (2012), Pagination: 109-111Abstract
Seed samples of Sword bean (Canavalia gladiata) (Jacq, DC) were analysed for phytohaemagglutinating (lectin) activity. The lectins of raw seed samples exhibited lectin activity without any specificity against human “A”, “B”, and “O” erythrocytes. Among the different post harvest treatments almost complete reduction in phytohaemagglutinating activity was recordedwhen the seed samples were subject to autoclaving followed by dry heat treatment (roasting). Nonetheless, soaking in salt (NaCl) solution was found to be ineffective in reducing lectin activity.