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Regupathi, Iyyaswami
- Screening of Reverse Micellar System for the Extraction of Bovine Lactoferrin
Abstract Views :508 |
PDF Views:190
Authors
Affiliations
1 Department of Chemical Engineering, National Institute of Technology Karnataka, Surathkal, Srinivasnagar Post-575025, Mangalore, Karnataka, IN
1 Department of Chemical Engineering, National Institute of Technology Karnataka, Surathkal, Srinivasnagar Post-575025, Mangalore, Karnataka, IN
Source
International Journal of Earth Sciences and Engineering, Vol 10, No 4 (2017), Pagination: 844-847Abstract
Lactoferrin (LF) is a multifunctional and an iron-binding protein (Mw∼77-80 kDa) present in animal milk. It is an important additive used in milk based infant formula, cosmetics, health supplements, oral care products due to its unique biological attributes. Present global demand of LF is estimated to be around 2, 62,000 kg/year. Reverse micellar extraction, a selective extraction, can be an alternate for chromatography and simulated moving bed (SMB) technology as it overcomes all the difficulties like high process cost and scale-up. Present work focuses on the selection of the suitable reverse micellar system by considering anionic (Bis (2-ethylhexyl) sulphosuccinate sodium salt), cationic (Cetyltrimethylammonium bromide) and non-ionic (Trition X 100) surfactants with organic solvents for the extraction of LF from the aqueous solution. All the RMSs were characterized (critical micelle concentration, water content, and micelle size) and their extraction efficiency was analysed. Cetyltrimethylammonium bromide with n-heptanol RMS was founded to offer 100% yield. The identified RMS may be further considered for the commercial extraction of LF from complex biological sources.Keywords
Lactoferrin, Reverse Micellar Extraction, Surfactants.Full Text
References
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Abstract Views :486 |
PDF Views:185
Authors
Affiliations
1 Department of Chemical Engineering, National Institute of Technology Karnataka, Mangalore-575025, IN
1 Department of Chemical Engineering, National Institute of Technology Karnataka, Mangalore-575025, IN
Source
International Journal of Earth Sciences and Engineering, Vol 10, No 4 (2017), Pagination: 863-867Abstract
Indian Sardine oil is a rich source of n-3 polyunsaturated fatty acids (n-3 PUFA) such as eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), which exists in the form of glycerides. The current work aims to optimize a process to enhance n-3 PUFA esters in the refined sardine oil by optimizing the enzymatic hydrolysis of glycerides having saturated fatty acid esters, using Pseudomonas cepacia lipase (PCL). Degree of hydrolysis (DOH) of PCL dissolved in solvent (polypropylene glycol) in the presence of surfactants like tween 80 and SDS was observed to be the highest at 40°C, 4 mg/mL enzyme load, surfactant to enzyme ratio of 2:1 (v/v), oil to water ratio of 1:1 (w/w), hydrolyzed for 15 minutes. After hydrolysis, oil was deacidified using methanolic extraction. Upon analysis of oil by using Gas Chromatography with FID, an increase in n-3 PUFA content from 17.91% (w/w) to 22.63% (w/w) was evidenced. RP-HPLC equipped with ELSD showed that most of the fatty acids exist in the form of monoglycerides.Keywords
Degree of Hydrolysis, Docosahexaenoic Acid, Eicosapentaenoic Acid, Indian Sardine Oil, Hydrolysis, Pseudomonas Cepacia Lipase.Full Text
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