Current Science

Rahul C. Salunkhe ¹, Dhiraj P. Dhotre ², Bipinchandra K. Salunke ¹, Vikas S. Patil ², Vaibhav Mahale ¹, Raymond J. Andrew ³, Milind S. Patole ¹, Ketan P. Narkhede ⁴, Yogesh S. Shouche ²

Affiliations
1 National Centre for Cell Science, University of Pune Campus, Ganeshkhind, Pune 411 007, IN
2 Microbial Culture Collection, National Centre for Cell Science, University of Pune Campus, Ganeshkhind, Pune 411 007, IN
3 Department of Zoology, Hislop College, Nagpur 440 001, IN
4 Department of Microbiology, Moolji Jaitha College, Jalgaon 425 002, IN

Abstract

Wolbachia are maternally inherited bacterial endo-symbionts of arthropods distributed among a wide range of hosts. It is now well known that they induce reproductive manipulations in their arthropod hosts by various phenotypic effects. The objective of the present study was to investigate Wolbachia infection among the insect order Odonata comprising 16 species from 5 families. Fifteen odonate species representing five families were found to harbour Wolbachia with the overall infection rate of 70%, out of which four-teen species are reported for the first time. According to multilocus sequence typing (MLST) data and phylo-genetic analysis, all odonate Wolbachia species belong to supergroup F, except Trithemis pallindinervis, which belongs to supergroup B. MLST data reveal 20 new, highly similar STs (99.32  0.34). We found a high rate of Wolbachia infection in Odonata of India, which indicates importance of this association. The charac-terization of these Wolbachia strains promises to lead to a deeper insight into this interaction, which is essential for further studies based on their phenotypic effects. The study suggests that all the characterized Wolbachia STs are totally new and arise as a result of point mutation.

Keywords

Multilocus Sequence Typing, Phenotypic Effects, Point Mutations.

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