Current Science

Aasaithambi Kalaiselvi ¹, Selvaraj Mohana Roopan ², Gunabalan Madhumitha ², C. Ramalingam ¹, Al-Dhabi Naif Abdullah ³, Mariadhas Valan Arasu ³

Affiliations
1 Industrial Biotechnology, School of Biosciences and Technology, VIT University, Vellore 632 014, IN
2 Chemistry of Heterocycles and Natural Product Research Laboratory, Department of Chemistry, School of Advanced Sciences, VIT University, Vellore 632 014, IN
3 Department of Botany and Microbiology, Addiriyah Chair for Environmental Studies, College of Science, King Saud University, Riyadh 11451, SA

Abstract

Catharanthus roseus leaf extract has potential efficien-cy for the formation of biosynthesized zinc oxide (ZnO) nanoparticles against dye degradation with various time intervals. The leaves of C. roseus were utilized as reducing agent for the formation of metal precursors into metal oxide nanoparticles. C. roseus-mediated ZnO nanoparticles were confirmed by vari-ous analytical techniques such as UV-Vis, FT-IR, XRD and morphological studies by SEM and TEM. Results show that green synthesized ZnO nanoparti-cles are directly proportional to reaction time. The phytoconstituents present in leaf extract of C. roseus play a major role in ZnO nanoparticles formation by acting as a reducing agent.

Keywords

Biological Synthesis, Catharanthus Roseus, Leaf Extract, Zinc Oxide Nanoparticles.

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Ruthiran Papitha ¹, Chinnadurai Immanuel Selvaraj ¹, V. Palanichamy ¹, Prabhakarn Arunachalam ², Selvaraj Mohana Roopan ³

Affiliations
1 School of Biosciences and Technology, VIT School of Agricultural Innovations and Advanced Learning, Vellore Institute of Technology, Vellore 632 014, IN
2 Electrochemistry Research Group, Chemistry Department, College of Science, King Saud University, Riyadh 11451, SA
3 Chemistry of Heterocycles and Natural Product Research Laboratory, Department of Chemistry, School of Advanced Science, Vellore Institute of Technology, Vellore 632 014, IN

Abstract

Kappaphycus alvarezii, marine red algae was collected from the Mandapam coastal region, Tamil Nadu, India to examine cytotoxic and redox capacity of its extracts. HPLC analysis indicates the presence of phenolic compounds in the methanolic extract. The maximum phenol content measured as mg of gallic acid standard equivalent was found to be 86.45 ± 70.72 mg/ml extract. The total flavonoid content was found to be 85.52 ± 32.57 mg/ml in the extract (using quercetin as a standard). The antioxidant potential of the extracts was determined by employing DPPH scavenging assay and antioxidant assay using the phosphomolybdenum method. Methanolic extract demonstrated efficient antioxidant capacity and DPPH radical scavenging activity. GC–MS analysis was carried out in crude extracts of K. alvarezii. MTT assay showed that the extracts are toxic to HeLa cells and their IC50 value was 1.75 mg/ml. The study shows that different extracts of K. alvarezii have strong antioxidant and less cytotoxic activity against HELA cells.

Keywords

Antioxidants, Cytotoxic Capacity, Kappaphycus alvarezii, Phytochemical Screening.

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