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Balasubramani, S. P.
- Establishing Taxonomic Identity and Selecting Genetically Diverse Populations for Conservation of Threatened Plants Using Molecular Markers
Abstract Views :289 |
PDF Views:93
Authors
N. K. Chrungoo
1,
G. R. Rout
2,
S. P. Balasubramani
3,
P. E. Rajasekharan
4,
K. Haridasan
3,
B. R. P. Rao
5,
R. Manjunath
3,
G. Nagduwar
3,
P. Venkatasubramanian
3,
A. Nongbet
1,
M. Hynniewta
1,
D. Swain
2,
S. Salamma
5,
K. Souravi
4,
S. N. Jena
6,
S. K. Barik
1
Affiliations
1 Department of Botany, North-Eastern Hill University, Shillong 793 022, IN
2 Department of Agricultural Biotechnology, College of Agriculture, Orissa University of Agriculture & Technology, Bhubaneswar 751 003, IN
3 Trans-Disciplinary University, Foundation for Revitalisation of Local Health Traditions, 74/2, Jarakabanade Kaval, Attur PO, Yelahanka, Bengaluru 560 106, IN
4 Division of Plant Genetic Resources, Indian Institute of Horticultural Research, Hessaraghatta Lake Post, Bengaluru 560 089, IN
5 Department of Botany, Biodiversity Conservation Division, Sri Krishnadevaraya University, Anantapur 515 003, IN
6 CSIR-National Botanical Research Institute, Lucknow 226 001, IN
1 Department of Botany, North-Eastern Hill University, Shillong 793 022, IN
2 Department of Agricultural Biotechnology, College of Agriculture, Orissa University of Agriculture & Technology, Bhubaneswar 751 003, IN
3 Trans-Disciplinary University, Foundation for Revitalisation of Local Health Traditions, 74/2, Jarakabanade Kaval, Attur PO, Yelahanka, Bengaluru 560 106, IN
4 Division of Plant Genetic Resources, Indian Institute of Horticultural Research, Hessaraghatta Lake Post, Bengaluru 560 089, IN
5 Department of Botany, Biodiversity Conservation Division, Sri Krishnadevaraya University, Anantapur 515 003, IN
6 CSIR-National Botanical Research Institute, Lucknow 226 001, IN
Source
Current Science, Vol 114, No 03 (2018), Pagination: 539-553Abstract
The extent of genetic diversity within a species is an important determinant of successful adaptation to adverse environmental conditions. Assessment of extent of genetic diversity/variability is also important to monitor genetic erosion within a species. In threatened plant species, genetic diversity assessment helps in selection of genetically diverse populations to enrich the genetically impoverished populations, thus minimizing the probability of genetic drift. Confirming taxonomic identity of threatened species, particularly those belonging to species complexes with dispute identity, is another essential task in the conservation of threatened species, which is best resolved through molecular approaches. The present study estimated the genetic variability within and among the populations of four threatened species, viz. Justicia beddomei (C.B. Clarke) Bennet (Acanthaceae), Embelia ribes Burm. f. (Myrsinaceae), Madhuca insignis (Radlk.) H.J. Lam (Sapotaceae) and Cycas beddomei Dyer (Cycadaceae) using Inter Simple Sequence Repeat (ISSR) and Simple Sequence Repeat (SSR) markers for selecting the genetically diverse populations. The phylogeny was analysed through ITS (nrDNA) and matK (cpDNA) sequences to confirm the species identity.
The phylogenetic analyses confirmed four distinct species of Justicia, which also revealed that J. beddomei and J. adhatoda were sister groups with a common ancestor showing rapid parallel speciation with J. gendarussa in one clade and J. betonica in another. Madhuca insignis with extremely small population in the Western Ghats (Karnataka to Kerala) might have undergone either extensive hybridization or incipient speciation. In case of Embelia species, a greater evolutionary closeness between E. subcoraceae and E. floribunda was revealed, while E. ribes had a distinct clad. Both ISSR and SSR markers distinguished various genotypes of Cycas beddomei.
Keywords
Conservation, Genetic Variability, Molecular Markers, Phylogeny, Threatened Plants.Full Text
References
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Abstract Views :272 |
PDF Views:73
Authors
Affiliations
1 TransDisciplinary University, Foundation for Revitalisation of Local Health Traditions, 74/2, Jarakabhande Kaval, Attur Post, via Yelahanka, Bengaluru 560 106, IN
2 G.B. Pant National Institute of Himalayan Environment and Sustainable Development, Kosi-Katarmal, Almora 263 643, IN
1 TransDisciplinary University, Foundation for Revitalisation of Local Health Traditions, 74/2, Jarakabhande Kaval, Attur Post, via Yelahanka, Bengaluru 560 106, IN
2 G.B. Pant National Institute of Himalayan Environment and Sustainable Development, Kosi-Katarmal, Almora 263 643, IN
Source
Current Science, Vol 114, No 03 (2018), Pagination: 554-561Abstract
Medicinal plants are used as a source of raw drugs, chemical compounds or bioactive metabolites. Many of the medicinal plant species are facing threat of extinction due to indiscriminate harvesting by humans. Conservation of such species is no longer an altruistic choice but a necessity to ensure sustainable supply of bioactive compounds to the drug industry. This article demonstrates that conservation of threatened species is possible through large-scale cultivation of elite germplasm identified using biochemical markers. Six species, viz. Aconitum balfourii Stapf, Aconitum heterophyllum Wall. ex Royle, Podophyllum hexandrum Royle (syn = Sinopodophyllum hexandrum (Royle) T. S. Ying), Picrorhiza kurroa Royle ex Berth., Berberis aristata DC. and Embelia ribes Burm. f. were selected for the study under the all-India coordinated project on threatened species. The approach proved to be effective for bringing back the species from the verge of extinction.Keywords
Bioactive Metabolites, Conservation Strategy, Medicinal Plants, Elite Germplasm.Full Text
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