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Thomas, Regi J.
- In Vitro Regeneration of Coconut Plantlets from Immature Inflorescence
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Authors
Affiliations
1 ICAR-Central Plantation Crops Research Institute, Regional Station, Kayamkulam, Alappuzha 690 533, IN
2 ICAR-Central Plantation Crops Research Institute, Kudlu PO, Kasaragod 671 124, IN
1 ICAR-Central Plantation Crops Research Institute, Regional Station, Kayamkulam, Alappuzha 690 533, IN
2 ICAR-Central Plantation Crops Research Institute, Kudlu PO, Kasaragod 671 124, IN
Source
Current Science, Vol 117, No 5 (2019), Pagination: 813-820Abstract
Clonal propagation of elite, disease-free coconut palms is a promising technique for producing uniform planting material with high yield and disease resistance. Over the past few decades cloning of coconut has been attempted in a number of laboratories worldwide; however, success has been limited. In the present study, immature inflorescences of 2–12 cm size were collected from West Coast Tall variety and the rachilla segments were cultured on four different media combinations in dark condition. White translucent outgrowths were maximum in Y3 medium supplemented with 4.54 μM 2,4-dichlorophenoxyacetic acid (92%) followed by medium 72 with 41.4 μM picloram, 61.8 μM putrescine and 4.54 μM thidiazuron (TDZ) (87%). After eight weeks in dark, shoot-like outgrowth was noticed more in Y3 III (65%) followed by Y3 I. After eight months dark incubation, the cultures were transferred to 1/2 Murashige and Skoog (MS) with two hormone combinations and high frequency of multiple shoot formation was noticed in 1/2 MS with 5.37 μM naphthalene acetic acid (NAA) and 4.44 μM 6-benzylaminopurine (BAP). Maximum shoot development was observed Y3 medium fortified with 5 μM 2-isopentenyl adenine (2ip) and 5 μM BAP. The individual shoots after development of 3–4 leaves were transferred to 1/2 Y3 medium supplemented with 5.37 μM NAA and 24.6 μM indole-3-butyric acid (IBA), and ischolar_main initiation was observed in 39.28% plantlets. Start codon targeted (SCoT) profiling based on banding pattern of PCR-amplified products confirmed the clonal fidelity of in vitro regenerated coconut plantlets. The study indicates the possibility of developing an in vitro regeneration protocol for coconut using immature inflorescence explants.Keywords
Clonal Fidelity, Coconut, in vitro Regeneration, Rachillae.Full Text
References
- Nguyen, O. T., Bandupriya, H. D. D., Villalobos, V. L., Sisunandar, S., Foale, M. and Adkins, S. W., Tissue culture and associated biotechnological interventions for the improvement of coconut (Cocos nucifera L.): a review. Planta, 2015, 242, 1059– 1076.
- Fernando, S. C., Vidhanaarachchi, V. R. M., Weerakoon, L. K. and Santha, E. S., What makes clonal propagation of coconut difficult? Asia-Pac. J. Mol. Biol. Biotechnol., 2010, 18(1), 163– 165.
- Branton, R. L. and Blake, J., Development of organized structures in callus derived from explants of Cocos nucifera L. Annu. Bot., 1983, 52, 673–678.
- Raju, C. R., Prakashkumar, P., Chandramohan, M. and Iyer, R. D., Coconut plants from leaf tissue cultures. J. Plant Crops, 1984, 12, 75–78.
- Buffard-Morel, J., Verdeil, J. L. and Pannetier, C., Embryogenese somatique du cocotier (Cocos nucifera L.) a partir de tissus foliaires: etude histologique. Can. J. Bot., 1992, 70, 735–741.
- Branton, R. L. and Blake, J., In vitro plantlet development from calloid derived from inflorescence of Cocos nucifera L. In Proceedings of International Congress Plant Tissue Cell Culture (eds Somers, D. A. et al.), University of Minnesota, Minneapolis, USA, 1986, p. 399 (abstr.).
- Verdeil, J. L., Huet, C., Grosdemange, R. and Buffard-Morel, J., Plant regeneration from cultured immature inflorescences of coconut (Cocos nucifera L.): evidence for somatic embryogenesis. Plant Cell Rep., 1994, 13, 218–221.
- Blake, J. and Hornung, R., Somatic embryogenesis in coconut (Cocos nucifera L.). In Somatic Embryogenesis in Woody Plants (eds Jain, S., Gupta, P. and Newton, R.), Kluwer, Dordrecht, The Netherlands, 1995, pp. 327–349.
- Raju, C. R., Direct in vitro shoot development from immature rachilla explants of coconut (Cocos nucifera L.). CORD, 2006, 22(1), 51–58.
- Karunaratne, S. and Periyapperuma, K., Culture of immaure embryos of coconut (Cocos nucifera L.): callus proliferation and somatic embryogenesis. Plant Sci., 1989, 62, 247–253.
- Hornung, R., Micropropagation of Cocos nucifera L. from plumular tissues excised from mature zygotic embryos. Plantation, Recherche, Dev., 1995, 50, 38–41.
- Chan, J. L., Saenz, L., Talavera, C., Hornung, R., Robert, M. and Oropeza, C., Regeneration of coconut (Cocos nucifera L.) from plumule explants through somatic embryogenesis. Plant Cell Rep., 1998, 17, 515–521.
- Rajesh, M. K., Radha, E., Sajini, K. K., Karun Anitha and Parthasarathy, V. A., Plant regeneration through organogenesis and somatic embryogenesis from plumular explants of coconut (Cocos nucifera L.). J. Plantation Crops, 2005, 33, 9–17.
- Rajesh, M. K., Radha, E., Sajini, K. K. and Karun Anitha, Polyamine-induced somatic embryogenesis and plantlet regeneration in vitro from plumular explants of dwarf cultivars of coconut (Cocos nucifera L.). Indian J. Agric. Sci., 2014, 84(4), 527–530.
- Shirke, S. V., Kendurkar, S. V, Apte, A. V, Phadke, C. H., Nadgauda, R. S. and Mascarenhas, A. F., Regeneration in coconut tissue culture In Advances in Coconut Research and Development (eds Nair, M. K. et al.), Oxford & IBH Publ. Co Pvt Ltd, New Delhi, 1993, pp. 235–237.
- Hornung, R. and Verdeil, J. L., Somatic embryogenesis in coconut from immature inflorescence explants. In Current Advances in Coconut Biotechnology (eds Oropeza, C. et al.), Kluwer, The Netherlands, 1999, pp. 297–308.
- Saenz, L., Souza, R., Chan, J. L., Hornung, R., Rillo, E., Verdeil, J. L. and Oropeza, C., Somatic embryogenesis and regeneration in coconut from plumular explants. In Current Advances in Coconut Biotechnology (eds Oropeza, C. et al.), Kluwar Academic Publishers, Dordrecht, The Netherlands, 1999, pp. 309–319.
- Oropeza, C., Cancino, G. S., Sáenz, L., Narváez, M., Rodríguez, G. and Chan, J. L., Coconut (Cocos nucifera L.) somatic embryogenesis using immature inflorescence explants. In Step Wise Protocols for Somatic Embryogenesis of Important Woody Plants (eds Jain, S. M. and Gupta, P.), Forestry Sciences, 2018, p. 85; https://doi.org/10.1007/978-3-319-79087-9_8.
- Eeuwens, C. J., Effects of organic nutrients and hormones on growth and development of tissue explants from coconut (Cocos nucifera L.) and date (Phoenix dactylifera) palms cultured in vitro. Physiol. Plant., 1976, 36, 23–28.
- Murashige, T. and Skoog, F., A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant., 1962, 15, 473–497.
- Collard, B. C. Y. and Mackill, D. J., Start codon targeted (SCoT) polymorphism: a simple, novel DNA marker technique for generating gene-targeted markers. Plant Mol. Biol. Rep., 2009, 27, 86–93.
- Rajesh, M. K., Sabana, A. A., Rachana, K. E., Shafeeq Rahman, Jerard, B. A. and Karun, Anitha, Genetic relationship and diversity among coconut (Cocos nucifera L.) accessions revealed through SCoT analysis. Biotech., 2015, 5, 999–1006.
- Gamborg, O. L., Miller, R. A. and Ojima, K., Nutrient requirements of suspension cultures of soyabean ischolar_main cells. Exp. Cell Res., 1968, 50, 151–158.
- Bhalla-Sarin, N., Bagga, S., Sopory, S. K. and Guha-Mukherjee, S., Induction and differentiation of callus from embryos of Cocos nucifera L. by IAA-conjugates. Plant Cell Rep., 1986, 5, 322–324.
- Fernando, S. C. and Gamage, C. K. A., Abscissic acid induced somatic embryogenesis in immature embryo expalnts of coconut. Plant Sci., 2000, 151, 193–198.
- Adkins, S. W., Samosir, Y. M. S., Ernawati, A., Godwin, I. D. and Drew, R. A., Control of ethylene and use of polyamines can optimise the conditions for somatic embryogenesis in coconut (Cocos nucifera L.) and papaya (Carica papaya L.). In Proceedings of the International Symposium of Biotechnology in Tropical and Subtropical Species (ed. Drew, R. A.), Brisbane, Australia, 1998, pp. 459–466.
- Samosir, Y. M. S., Optimisation of somatic embryogenesis in coconut (Cocos nucifera L.), Ph D thesis, The University of Queensland, Australia, 1999.
- Vidhanaarachchi, V. R. M. and Weerakoon, L. K., Callus induction and direct shoot formation in in vitro cultured immature inflorescence tissues of coconut. COCOS, 1997, 12, 39–43.
- Fernando, S. C. and Gamage, C. K., Clonal propagation of coconut: improved culture conditions for rhyzogenesis. COCOS, 1994–1995, 10, 20–25.
- Mgbeze, G. C. and Iserhienrhien, A., Somaclonal variation associated with oil palm (Elaeis guineensis Jacq.) clonal propagation: a review. Afr. J. Biotechnol., 2014, 13(9), 989–997.
- Larkin, P. J. and Scowcroft, W. R., Somaclonal variation – a novel source of variability from cell cultures for plant improvement. Theor. Appl. Genet., 1981, 60(4), 197–214.
- Fernando, S. C. et al., Genetic fidelity and ex vitro performance of tissue-cultured coconut plants. In Proceedings of the International Conference of the Coconut Research Institute of Sri Lanka – Part II (abstr.), (eds Peiris, T. S. G. and Ranasinghe, C. S.), Coconut Research Institute, Lunuwila, Sri Lanka, p. 19, 2004.
- Bandupriya, H. D. D., Iroshini, W. W. M. A., Perera, S. A. C. N., Vidhanaarachchi, V. R. M., Fernando, S. C., Santha, E. S. and Gunathilake, T. R., The genetic fidelity testing using SSR marker assay confirms trueness to type of micropropagated coconut (Cocos nucifera L.) plantlets derived from unfertilized ovaries. Open Plant Sci. J., 2017, 10, 46–54.