Refine your search
Collections
Co-Authors
Journals
Year
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z All
Qadri, Rashad
- A Modified Protocol for Total Rna Isolation from Different Oil Palm (Elaeis guineensis) Tissues using Cetyltrimethylammonium Bromide
Abstract Views :436 |
PDF Views:121
Authors
Affiliations
1 Hainan Key Laaboratory of Tropical Oil Crops Biology/Coconut Research Institute, Chinese Academy of Tropical Agricultural Science, Wenchang, Hainan 571339, CN
2 Department of Environmental Sciences, Lahore College for Women University Lahore, Lahore 54600, PK
3 Institute of Horticultural Sciences, University of Agriculture, Faisalabad, PK
1 Hainan Key Laaboratory of Tropical Oil Crops Biology/Coconut Research Institute, Chinese Academy of Tropical Agricultural Science, Wenchang, Hainan 571339, CN
2 Department of Environmental Sciences, Lahore College for Women University Lahore, Lahore 54600, PK
3 Institute of Horticultural Sciences, University of Agriculture, Faisalabad, PK
Source
Current Science, Vol 116, No 3 (2019), Pagination: 479-482Abstract
Extraction of high-quality RNA from oil palm tissues is challenging due to the presence of polysaccharides, polyphenols and other complexes that co-precipitate with RNA. Therefore, isolation of high-quality RNA from oil palm is challenging due to the presence of varying amounts of these constituents in diverse tissues. This communication describes a modified RNA extraction protocol based on the cetyltrimethylammonium bromide (CTAB) method which is useful for extracting high-quality RNA from different oil palm tissues. Total RNA isolation using a modified CTAB protocol was compared with two different methods, a conventional TRIzol method and the method for RNA isolation from palms (MRIP). Both methods were useful for isolating RNA from leaf tissues; however, they were not effective in isolating RNA from other tissues. The current protocol based on a modified CTAB method was efficient in isolating high-quality total RNA from oil palm fruit tissues, including mesocarp and endosperm, stem, ischolar_main and flower tissues. This modified CTAB-based protocol gave approximately 20-30 μg of total RNA from 150 mg of tissue within 5-6 h.Keywords
CTAB Method, Gene Expression, Oil Palm, RNA Extraction.References
- Alain, R. and Larkins, B. A., Biotechnology in Agriculture and Forestry, Springer-Verlag, Heidelberg, Germany, 2007, vol. 63, pp. 59-80.
- Nair, P., Oil palm (Elaeis guineensis Jacquin). In The Agronomy and Economy of Important Tree Crops of the Developing World, Elsevier Inc., London, UK, 2010, pp. 209-236.
- Singh, R. P., Hakimi Ibrahim, M. Norizan Esa and Iliyana, M. S., Composting of waste from palm oil mill: a sustainable waste management practice. Rev. Environ. Sci. Biotechnol., 2010, 9, 331- 344.
- Sambanthamurthi, R., Singh, R., Kadir, A. P. G., Abdullah, M. O. and Kushairi, A., Opportunities for the oil palm via breeding and biotechnology. In Breeding Plantation Tree Crops: Tropical Species, Springer, 2009, pp. 377-421.
- Baud, S., Mendoza, M. S., To, A., Harscoët, E., Lepiniec, L. and Dubreucq, B., WRINKLED1 specifies the regulatory action of leafy cotyledon2 towards fatty acid metabolism during seed maturation in arabidopsis. Plant J., 2007, 50, 825-838.
- Maeo, K. et al., An ap2-type transcription factor, wrinkled1, of arabidopsis thaliana binds to the aw-box sequence conserved among proximal upstream regions of genes involved in fatty acid synthesis. Plant J., 2009, 60, 476-487.
- Helen, H. T., Pelletier, C. and Beardmore, T., Total RNA isolation from Picea mariana dry seed. Plant Mol. Biol. Rep., 2004, 22, 93a-93e.
- Wang, X., Tian, W. and Li, Y., Development of an efficient protocol of rna isolation from recalcitrant tree tissues. Mol. Biotechnol., 2008, 38, 57-64.
- Salzman, R. A., Fujita, T., Zhu-Salzman, K., Hasegawa, P. M. and Bressan, R. A., An improved RNA isolation method for plant tissues containing high levels of phenolic compounds or carbohydrates.
- Plant Mol. Biol. Rep., 1999, 17, 11-17.
- Li, J.-H., Tang, C.-H., Song, C.-Y., Chen, M.-J., Feng, Z.-Y. and Pan, Y.-J., A simple, rapid and effective method for total RNA extraction from Lentinula edodes. Biotechnol. Lett., 2006, 28, 1193-1197.
- Chun Jun, Z. Y.-F., Sheng-Hua, W. and Fang, C., A RNA isolation method suitable for a wide range of materials. Prog. Biochem. Biophys., 2008, 35, 591-597.
- Thanh, T., Omar, H., Abdullah, M. P., Chi, V. T. Q., Noroozi, M., Ky, H. and Napis, S., Rapid and effective method of RNA isolation from green microalga Ankistrodesmus convolutus. Mol. Biotechnol., 2009, 43, 148-153.
- Takahashi, H. et al., A method for obtaining high quality RNA from paraffin sections of plant tissues by laser microdissection. J. Plant Res., 2010, 123, 807-813.
- Tattersall, E. A., Ergul, A., AlKayal, F., DeLuc, L., Cushman, J. C. and Cramer, G. R., Comparison of methods for isolating high-quality RNA from leaves of grapevine. Am. J. Enol. Viticult., 2005, 56, 400-406.
- Hou, P., Xie, Z., Zhang, L., Song, Z., Mi, J., He, Y. and Li, Y., Comparison of three different methods for total RNA extraction from Fritillaria unibracteata, a rare Chinese medicinal plant. J. Med. Plants Res., 2011, 5, 2835-2839.
- Kiefer, E., Heller, W. and Ernst, D., A simple and efficient protocol for isolation of functional RNA from plant tissues rich in secondary metabolites. Plant Mol. Biol. Rep., 2000, 18, 33-39.
- Bourgisa, F., Kilaruc, A., Caod, X., Ngando-Ebonguee, G.-F., Driraf, N., Ohlrogged, J. B. and Arondela, V., Comparative transcriptome and metabolite analysis of oil palm and date palm mesocarp that differ dramatically in carbon partitioning. Metabolism, 2011, 156, 564-584.
- Tranbarger, T. J. et al., Regulatory mechanisms underlying oil palm fruit mesocarp maturation, ripening, and functional specialization in lipid and carotenoid metabolism. Plant Physiol., 2011, 156, 564-584.
- Xiao, Y. et al., Efficient isolation of high quality RNA from tropical palms for RNA-Seq analysis. Plant Omics, 2012, 5, 584.
- Bernardo, G. D., Gaudio, S. D., Galderisi, U., Cascino, A. and Cipollaro, M., Comparative evaluation of different DNA extraction procedures from food samples. Biotechnol. Prog., 2007, 23, 297-301.
- Wilfinger, W. W., Mackey, K. and Chomczynski, P., Effect of pH and ionic strength on the spectrophotometric assessment of nucleic acid purity. BioTechniques, 1997, 22, 474-481.