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Authors
Affiliations
1 Department of Molecular Biology and Genetic Engineering, College of Basic Sciences and Humanities, G.B. Pant University of Agriculture and Technology, Pantnagar, US Nagar 263 145, IN
2 Department of Veterinary Pharmacology & Toxicology, College of Veterinary & Animal Sciences, G.b. Pant University of Agriculture and Technology, Pantnagar, US Nagar 263 145,, IN
Source
Current Science, Vol 109, No 7 (2015), Pagination: 1320-1326
Abstract
The present study is an attempt to determine the combined hepatoprotective potential of hydroethanolic leaf extracts of Murraya koenigii and Phyllanthus niruri against paracetamol (PCM) and ethanolinduced toxicity in human hepatoma HepG2 cell line. Toxicity in cells was induced by treatment with 15 mM PCM and 50 mM ethanol for 24 h as manifested by a significant (P < 0.05) decrease in cell viability, increase in the leakage of serum glutamate oxaloacetate transaminase and serum glutamate pyruvate in culture medium, increase in lipid peroxidation and reduction in reduced glutathione in cell lysate. These alterations were significantly ameliorated when cells were treated with a combination of hydroethanolic leaf extracts of M. koenigii and P. niruri, and silymarin during both prophylactic and curative studies. Both post-treatment (curative) and pre-treatment (prophylactic) with the combination of plant extracts were able show effective hepatoprotection. This was also evident during morphological studies. The combination of plant extracts thus holds immense potential for future use as a hepatoprotectant.
Keywords
Ethanol, Hepatoprotection, HepG2 Cell Line, Murraya Koenigii, Paracetamol, Phyllanthus Niruri.
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