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Javaregowda, Palaksha Kanive
- Significance of altered glycosyltransferase expression levels in oral cancer
Abstract Views :167 |
PDF Views:97
Authors
Mallikarjun Goni
1,
Anil Kumar Desai
2,
Niranjan Kumar
2,
Veda Hegde
3,
Palaksha Kanive Javaregowda
4,
D. B. M. Virupakshaiah
5
Affiliations
1 SDM Research Institute for Biomedical Sciences, A Constituent Unit of Shri Dharmasthala Manjunatheshwara University, 5th Floor, Manjushree Building, Sattur, Dharwad 580 009, Department of Biotechnology, Basaveshwar Engineering College, Vidyagiri, Bagalkot 587 102, IN
2 SDM Craniofacial Surgery and Research Centre, A Constituent unit of Shri Dharmasthala Manjunatheshwara University, Sattur, Dharwad 580 009, IN
3 Department of Oral Pathology, SDM College of Dental Sciences and Hospital, A Constituent Unit of Shri Dharmasthala Manjunatheshwara University, Sattur, Dharwad 580 009, IN
4 SDM Research Institute for Biomedical Sciences, A Constituent Unit of Shri Dharmasthala Manjunatheshwara University, 5th Floor, Manjushree Building, Sattur, Dharwad 580 009, IN
5 Department of Microbiology, Davangere University, Shivagangotri, Davangere 577 002, IN
1 SDM Research Institute for Biomedical Sciences, A Constituent Unit of Shri Dharmasthala Manjunatheshwara University, 5th Floor, Manjushree Building, Sattur, Dharwad 580 009, Department of Biotechnology, Basaveshwar Engineering College, Vidyagiri, Bagalkot 587 102, IN
2 SDM Craniofacial Surgery and Research Centre, A Constituent unit of Shri Dharmasthala Manjunatheshwara University, Sattur, Dharwad 580 009, IN
3 Department of Oral Pathology, SDM College of Dental Sciences and Hospital, A Constituent Unit of Shri Dharmasthala Manjunatheshwara University, Sattur, Dharwad 580 009, IN
4 SDM Research Institute for Biomedical Sciences, A Constituent Unit of Shri Dharmasthala Manjunatheshwara University, 5th Floor, Manjushree Building, Sattur, Dharwad 580 009, IN
5 Department of Microbiology, Davangere University, Shivagangotri, Davangere 577 002, IN
Source
Current Science, Vol 123, No 1 (2022), Pagination: 52-58Abstract
We evaluate the relative quantification of mRNA expression levels of the gene encoding an enzyme that catalyses initial step in glycosylation of dolichol-linked oligosaccharide such as dolichyl-phosphate N-acetylglucosaminephosphotransferase 1 (DPAGT1). Furthermore, beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase (MGAT3) and alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase (MGAT5) mediated N-glycosylation, selected a2, 3, a2, 6 sialyltransferases families and sialidase by using the Real-Time Quantitative Reverse Transcription PCR (qRT-PCR). Thirty human oral cancer tissue samples and twelve control samples were examined. Results indicated significant upregulation of ST6 N-acetylgalactosaminide alpha-2,6-sialyltransferase 1 (ST6GALNAC1) in oral cancer compared to controls. Additionally, the expressions of DPAGT1, MGAT3, MGAT5, ST3 beta-galactoside alpha-2,3-sialyltransferase 1 (ST3GAL1), ST6 beta-galactoside alpha-2,6-sialyltransferase 1 (ST6GAL1), and ST6GALNAC1 were considerably higher in the tumour in tongue lesion compared to the buccal cavity. Hence, our experiment demonstrates significance of glycosyltransferases in the progression of oral cancerKeywords
Aberrant Glycosylation, Invasion, Metastasis, Oral Cancer, SialyltransferaseFull Text
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