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Panneerselvam, P.
- A New Modified Nutrient Media for Cultivation of Actinobacteria
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PDF Views:79
Authors
Affiliations
1 Division of Soil Science, ICAR-Indian Institute of Horticultural Research, Hessaraghatta, Bengaluru 560 089, IN
1 Division of Soil Science, ICAR-Indian Institute of Horticultural Research, Hessaraghatta, Bengaluru 560 089, IN
Source
Current Science, Vol 120, No 4 (2021), Pagination: 718-722Abstract
The growth of Actinobacteria was evaluated in a new modified nutrient agar media (MNA) supplemented with 1% sodium chloride (NaCl) and compared with the universally accepted media which are under regular use. Thirteen different Actinobacterial isolates were evaluated for their growth in both solid and liquid media in MNA along with four regularly used media ISP-2, kenknight, starch casein and nutrient media. Results indicated that eight isolates (Streptomyces bulli, Streptomyces griseorubens, Streptomyces viridobrunneus, Streptomyces A3, Streptomyces A7, Streptomyces A11, Streptomyces A12 and Streptomyces A13), out of thirteen isolates could grow significantly faster on MNA compared with other solid agar media. Similarly, growth in liquid media recorded by optical density (OD) indicated that three isolates (S. griseorubens, S. viridobrunneus and A7) showed significantly higher OD followed by ISP-2, nutrient, starch casein and kenknight broth after seven days of incubation. All the isolates except S. viridobrunneus and Streptomyces A12 could tolerate NaCl concentration upto 6% in liquid modified nutrient broth. The present study showed that Actinobacteria can be successfully grown faster with full pigmentation at 1% NaCl concentration and they can tolerate up to 5% NaCl concentration, in the new proposed MNA media.Keywords
Actinobacteria, Modified Nutrient Agar Media, Optical Density, Sodium Chloride.Full Text
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- Molecular diversity of Nilaparvata lugens (Stål.) (Hemiptera: Delphacidae) from India based on internal transcribed spacer 1 gene
Abstract Views :167 |
PDF Views:78
Authors
Govindharaj Guru-Pirasanna-Pandi
1,
Aashish Kumar Anant
1,
Jaipal Singh Choudhary
2,
Soumya Bharati Babu
1,
G. Basana-Gowda
1,
M. Annamalai
1,
Naveenkumar Patil
1,
Totan Adak
1,
P. Panneerselvam
1,
Prakash Chandra Rath
1
Affiliations
1 Division of Crop Protection, ICAR-National Rice Research Institute, Cuttack 753 006, IN
2 ICAR-RCER Farming System Research Centre for Hill and Plateau Region, Ranchi 834 010, IN
1 Division of Crop Protection, ICAR-National Rice Research Institute, Cuttack 753 006, IN
2 ICAR-RCER Farming System Research Centre for Hill and Plateau Region, Ranchi 834 010, IN
Source
Current Science, Vol 122, No 12 (2022), Pagination: 1392-1400Abstract
Brown planthopper, Nilaparvata lugens, is the major pest of rice in India and causes significant yield loss. It causes damage by sucking the plant sap leading to a characteristic symptom called ‘hopper burn’. The present study was undertaken to assess the genetic variability of N. lugens populations from different rice ecologies in India, to comprehend and assist in planning proper management strategies. We evaluated the molecular diversity in 17 N. lugens populations based on internal transcribed spacer 1 (ITSI) gene sequences. In all, 53 unique haplotypes were identified and their numbers varied from 1 to 10 in the sampled populations. Genetic diversity indices like nucleotide diversity, haplotype number, haplotype diversity and average number of nucleotide differences revealed low to high levels of genetic diversity among the populations. A highly significant negative relation of Fu’s F and Tajima’s D tests with insignificant sum of square deviation (SSD) values indicated possible recent expansion of N. lugens in different Indian regions with a population expansion time of 3.9 million years. A non-significant correlation in isolation pattern by distance indicated that geographic barriers present in India are inadequate to bring genetic differentiation among N. lugens from different migratory populations. In the present study, the ITSI gene sequence was used to analyse genetic structure among N. lugens in India.Keywords
Genetic Structure, Haplotypes, Molecular Diversity, Nilaparvata Lugens, RiceFull Text
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