Current Science

Mandakini A. Shinde ¹, Ottoor Divya ¹

Affiliations
1 Department of Chemistry, Savitribai Phule Pune University, Ganeshkhind Road, Pune 411 007, IN

Abstract

Establishing precise and accurate analytical methods for multicomponent analysis using time- and cost-effective ways are highly advantageous. Here, syn-chronous fluorescence spectroscopic method is coupled with chemometric tools in order to achieve simultaneous quantitative analysis of a three-component system consisting of metoprolol, propranolol and amiloride. Chemometric methods such as principal component regression and partial least squares re-gression were applied to the fluorescence data. Root mean square error values, correlation coefficient and limit of detection were used to judge the potential of the model for prediction. The proposed method worked well for simultaneous analysis of the three-drug system in the presence of strong serum albumin signals.

Keywords

Three-Drug System, Partial least Squares Regression, Principal Component Regression, Synchronous Fluorescence Spectroscopy.

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V. Roshni ¹, Ottoor Divya ¹

Affiliations
1 Department of Chemistry, Savitribai Phule Pune University, Ganeshkhind Road, Pune 411 007, IN

Abstract

The present study focuses on the green synthesis of nitrogen-doped carbon dots (C.dots) from sesame seeds using microwave pyrolysis method. The C.dots obtained were characterized and extensively studied using transmission electron microscopy (TEM), UV/ visible spectroscopy, fluorescence spectroscopy and Fourier transform infra-red spectroscopy (FTIR) techniques. The results indicated the presence of highly fluorescent, aqueous soluble and significantly photostable C.dots with a quantum yield of 8.02%. The average size distributions of C.dots were found to be 5 nm. These C.dots were effectively applied as a selective sensor for Fe(III) as the fluorescence intensity was significantly quenched with increasing metal concentrations. The limit of detection (LOD) was found to be 2.56 M of Fe(III). This study demonstrates a low cost, environmental friendly and waste recyclable synthetic method for preparation of C.dots and its application as a selective Fe(III) sensor.

Keywords

Carbon Dots, Fluorescence, Sesame Seeds, Microwave Pyrolysis, Metal Ions, Quenching.

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