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Eswaran, Muthulakshmi
- Development of a sensitive and single-step PCR-based assay for detection of sandal spike phytoplasma
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Authors
Muthulakshmi Eswaran
1,
Karthick Thangaraj
1,
Balasubramanian Aiyar
1,
Suma Arun Dev
2,
Swathi Balakrishnan
2,
Modhumita Ghosh Dasgupta
1
Affiliations
1 Institute of Forest Genetics and Tree Breeding, R.S. Puram, Coimbatore 641 002, India, IN
2 Kerala Forest Research Institute, Peechi, Thrissur 680 653, India, IN
1 Institute of Forest Genetics and Tree Breeding, R.S. Puram, Coimbatore 641 002, India, IN
2 Kerala Forest Research Institute, Peechi, Thrissur 680 653, India, IN
Source
Current Science, Vol 122, No 12 (2022), Pagination: 1454-1458Abstract
Santalum album commonly known as Indian sandalwood, is extensively exploited due to its demand in the international market for essential oil. In the Indian subcontinent, the species is approaching commercial extinction due to overexploitation and sandal spike disease caused by phytoplasma. Molecular detection using PCR and RFLP is reported for disease diagnosis. In the present study, a single-step, stringent PCR-based assay was developed to detect the sandal spike phytoplasma. The titre estimation revealed that the marker maintained detection sensitivity up to 0.8 ng of template DNA. Subsequent to validation of the marker in a larger number of symptomatic individuals, this diagnostic assay can be routinely used to detect the pathogen. It will facilitate early detection and enable plantation managers to take adequate measures to control the spread of the disease.Keywords
Control Measures, Diagnostic Markers, Nested PCR, Pathogen, Phytoplasma, Sandal Spike DiseaseFull Text
References
- Rashkow, E. D., Perfumed the axe that laid it low: the endangerment of sandalwood in southern India. Indian Econ. Soc. Hist. Rev., 2014, 51, 41–70.
- Thomas, S., Detection of sandal spike phytoplasma using immunological and molecular techniques. Cochin University of Science and Technology, Kochi, 2000.
- Teixeira da Silva, Jaime, A., Kher, M. M., Soner, D. and Nataraj, M., Sandalwood spike disease: a brief synthesis. Environ. Exp. Bot., 2016, 14, 199–204.
- Weintraub, G. and Beanland, L., Insect vectors of phytoplasmas. Annu. Rev. Entomol., 2006, 51, 91–111.
- Mondal, S., Sundararaj, R. and Rao, Y., A critical appraisal on the recurrence of sandalwood spike disease and its management practices. For. Pathol., 2020, 50, e12648.
- Thomas, S. and Balasundaran, M., In situ detection of phytoplasma in spike disease-affected sandal using DAPI stain. Curr. Sci., 1998, 74, 989–993.
- Thomas, S. and Balasundaran, M., Purification of sandal spike phytoplasma for the production of polyclonal antibody. Curr. Sci., 2001, 80, 1489–1494.
- IPPC, DP 12. Phytoplasmas. Annex 12 to International Standard for Phytosanitary Measures (ISPM) 27. Diagnostic Protocols For Regulated Pests, Rome, IPPC/FAO, 2016.
- Lebsky, V., Poghosyan, A. and Silva-Rosales, L., Application of Scanning electron microscopy for diagnosing phytoplasmas in single and mixed (virus-phytoplasma) infection in papaya. Julius-KuhnArchiv., 2010, 427, 70–78.
- Khan, J. A. and Ahmad, J., Characterization and phylogeny of a phytoplasma inducing sandal spike disease in sandal (Santalum album). Ann. Appl. Biol., 2008, 153, 365–372.
- Mahesh, H. et al., Multi-omics driven assembly and annotation of the sandalwood (Santalum album) genome. Plant Physiol., 2018, 176, 2772–2788.
- IRPCM, ‘Phytoplasma’, a taxon for the wall-less, non-helical prokaryotes that colonise plant phloem and insects. Int. J. Syst. Evol. Microbiol., 2004; https://doi/101099/ijs002854.
- Kumari, S., Nagendran, K., Rai, A. B., Singh, B., Rao, G. P. and Bertaccini, A., Global status of phytoplasma diseases in vegetable crops. Front. Microbiol., 2019, 10, 1349.
- Marcone, C., Franco-Lara, L. and Toševski, I., Major phytoplasma diseases of forest and urban trees In Phytoplasmas: Plant Pathogenic Bacteria – I (eds Rao, G. et al.), Springer, Singapore, 2018.
- Linck, H., Kruger, E. and Reineke, A., A multiplex TaqMan qPCR assay for sensitive and rapid detection of phytoplasmas infecting Rubus species. PLoS ONE, 2017, 12, e0177808.
- Christensen, N. M., Nyskold, H. and Nicolaisen, M., Real-time PCR for universal phytoplasma detection and quantification. In Phytoplasma. Methods in Molecular Biology (Methods and Protocols) (eds Dickinson, M. and Hodgetts, J.), Humana Press, New Jersey, USA, 2013, vol. 938, pp. 245–252.
- Le, D. T. and Vu, N. T., Progress of loop-mediated isothermal amplification technique in molecular diagnosis of plant diseases. Appl. Biol. Chem., 2017, 60, 169–180.
- Kirdat, K., Sundararaj, R., Mondal, S., Reddy, K. M., Thorat, V. and Yadav, A., Novel aster yellows phytoplasma subgroup associated with sandalwood spike disease in Kerala, India. Phytopathogenic Mollicutes, 2019, 9, 33–34.
- Sundararaj, R., Kirdat, K., Mondal, S., Reddy, K. M., Thorat, V. and Yadav, A., A threat to sandalwood cultivation in the naturalised Marayoor sandalwood reserve (Kerala, India) through single and mixed phytoplasma infections. Phytopathogenic Mollicutes, 2020, 10, 89–95.
- Khan, J. A., Srivastava, P. and Singh, S. K., Identification of a ‘Phytoplasma asteris’-related strain associated with spike disease of sandal (Santalum album) in India. New Dis. Rep., 2006, 12, 44.