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Iqbal, Muhammad Shehzad
- In Silico Plum Pox Virus Silencing Via Host-retrieved miRNAs in Peach Plant
Abstract Views :184 |
PDF Views:77
Authors
Muhammad Naveed Shahid
1,
Syeda Shehnaz
1,
Muhammad Shehzad Iqbal
2,
Aneela Shabbeer
1,
Adil Jamal
3,
Sana Khalid
4
Affiliations
1 Department of Botany, Division of Science and Technology, University of Education, Lahore, PK
2 Centre of Excellence in Molecular Biology, University of the Punjab, Lahore, PK
3 Science and Research College of Nursing, Ummal Qura University, Makkah, SA
4 Department of Botany, Lahore College for Woman University, Lahore, PK
1 Department of Botany, Division of Science and Technology, University of Education, Lahore, PK
2 Centre of Excellence in Molecular Biology, University of the Punjab, Lahore, PK
3 Science and Research College of Nursing, Ummal Qura University, Makkah, SA
4 Department of Botany, Lahore College for Woman University, Lahore, PK
Source
Current Science, Vol 121, No 10 (2021), Pagination: 1316-1322Abstract
Peach (Prunus persica) is a deciduous, edible, stone fruit producing plant, belonging to the family Rosaceae. The plant is prone to various pathogens and one of them is the plum pox virus (PPV). This is a lethal virus of peach plant causing pox disease of plum. Its attack results in 83%–100% yield loss in highly susceptible varieties of peach plant. The complete genome of PPV is 9791 base pairs with positive-sense single strand. The full-length genome of PPV encodes a large polyprotein initially, which cleaves proteolytically into ten mature proteins – coat protein, helper component proteinase, P1, P3, viral genome-linked protein, 6K1, 6K2, cylindrical inclusion protein, cylindrical inclusion protein b and NIa-pro-proteins. The objective of this study is to identify such sites in the PPV genome which can be targeted by PPV-derived miRNAs through target prediction computational tools/algorithms. A total of 214 mature miRNAs were chosen from the miRNA database to check their complementarity with the PPV genome. Minimum free energy, folding energy, seed pairing, target-site accessibility, pattern recognition and multiple target sites were the parameters considered for target prediction algorithms. Two out of 214 miRNAs were predicted as potential against plum pox virus by three of four tools used for target prediction. Thus, the results encourage generating transgenic, PPV-resistant peach plants by expression of predicted miRNAs.Keywords
miRNAs, Peach Plant, Plum Pox Virus, Yield Loss, Target Prediction Algorithms.Full Text
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