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Das, Meera C.
- Artificial Seed for Short-Term Storage:Using Nodal Buds in Aquilaria malaccensis Lam
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Authors
Affiliations
1 Plant Biotechnology Laboratory, Department of Botany, North-Eastern Hill University, Shillong 793 022, IN
1 Plant Biotechnology Laboratory, Department of Botany, North-Eastern Hill University, Shillong 793 022, IN
Source
Current Science, Vol 115, No 11 (2018), Pagination: 2103-2109Abstract
Artificial seed technology is known to be the best alternative for short-term storage of plant germplasm in recalcitrant species. Aquilaria malaccensis, being a recalcitrant seeded tree, possesses seasonal and highly desiccation-sensitive seeds which lose viability with slight decrease in their moisture content, hence storage becomes a difficult task. The present study deals with short-term storage of A. malaccensis nodal buds through artificial seed technology. Nodal buds encapsulated with 2.5% sodium alginate and 100 mM calcium chloride, and polymerized for 40 min were found suitable for artificial seed preparation. Maximum regeneration rate of 83.3% and 75.0% was observed from encapsulated nodal buds stored at 4°C and 23 ± 2°C respectively, for 10 days. Storage was possible for a period of 60 days at 4°C and 50 days at 23 ± 2°C with average regeneration rate of 8.3% and 16.7% respectively.Keywords
Artificial Seeds, Encapsulation, Recalcitrant, Storage, Temperature.Full Text
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