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Vasanthakumari, M. M.
- Endophyte Fungal Diversity in Nothapodytes nimmoniana along its Distributional Gradient in the Western Ghats, India:Are Camptothecine (Anticancer Alkaloid) Producing Endophytes Restricted to Specific Clades?
Abstract Views :267 |
PDF Views:85
Authors
Singh Shweta
1,
B. R. Gurumurthy
2,
M. M. Vasanthakumari
1,
G. Ravikanth
3,
S. Dayanandan
4,
R. Storms
4,
M. B. Shivanna
5,
R. Uma Shaanker
1
Affiliations
1 School of Ecology and Conservation and Department of Crop Physiology, University of Agricultural Sciences, GKVK, Bengaluru 560 065, IN
2 Department of Crop Physiology, University of Agricultural and Horticultural Sciences, Shivamogga 577 201, IN
3 Ashoka Trust for Research in Ecology and the Environment, Royal Enclave, Srirampura, Jakkur PO, Bengaluru 560 064, IN
4 Centre for Structural and Functional Genomics and Biology Department, Concordia University, 7141 Sherbrooke West, Montreal, Quebec, H4B 1R6, CA
5 Department of Applied Botany, Kuvempu University, Jnana Sahyadri, Shankaraghatta, Shivamogga 577 451, IN
1 School of Ecology and Conservation and Department of Crop Physiology, University of Agricultural Sciences, GKVK, Bengaluru 560 065, IN
2 Department of Crop Physiology, University of Agricultural and Horticultural Sciences, Shivamogga 577 201, IN
3 Ashoka Trust for Research in Ecology and the Environment, Royal Enclave, Srirampura, Jakkur PO, Bengaluru 560 064, IN
4 Centre for Structural and Functional Genomics and Biology Department, Concordia University, 7141 Sherbrooke West, Montreal, Quebec, H4B 1R6, CA
5 Department of Applied Botany, Kuvempu University, Jnana Sahyadri, Shankaraghatta, Shivamogga 577 451, IN
Source
Current Science, Vol 109, No 1 (2015), Pagination: 127-138Abstract
Nothapodytes nimmoniana Graham (Icacinaceae) is a small tree distributed along a 1600 km mountain range in the Western Ghats biodiversity hotspot in southern India. The stem wood of N. nimmoniana accumulates high concentration (about 0.3% by dry weight) of the anti-cancer alkaloid, camptothecine (CPT). Several endophytic fungi isolated from this plant have been shown to produce camptothecine in vitro. In this study, we examined the diversity and distribution of fungal endophytes of N. nimmoniana along its entire distributional range in the Western Ghats and investigated if the CPT-producing endophytes are restricted to any specific clade. The leaf and stem of N. nimmoniana were sampled from 18 sites along the Western Ghats spanning 8-18°N lat. Endophytes were recovered from all sites with the colonization frequency ranging from 0% to 52% across the sites. One hundred and four endo-phytic fungal isolates were recovered from 118 plants and characterized both morphologically and by sequencing the internally transcribed spacer region of the nuclear rDNA gene. The fungal isolates belonged to 45 species (44 Ascomycetes and one Basidiomycetes). Fusarium and Hypoxylon were the most predominant genera comprising over half of the total isolates. Interestingly, CPT-producing endophytes were not restricted to any specific clade. We discuss these results in the context of the growing interest in endophytic fungi as possible alternative sources of plant secondary metabolites.Keywords
Camptothecine, Endophytic Fungi, Fungal Diversity and Distribution, Nothapodytes nimmoniana.Full Text
- Endophytic Fungi of Salt Adapted Ipomea pes-caprae L. R. Br: their Possible Role in Inducing Salinity Tolerance in Paddy (Oryza sativa L.)
Abstract Views :245 |
PDF Views:86
Authors
Affiliations
1 Department of Crop Physiology, University of Agricultural Sciences, GKVK, Bengaluru 560 065, IN
2 School of Ecology and Conservation, University of Agricultural Sciences, GKVK, Bengaluru 560 065, IN
1 Department of Crop Physiology, University of Agricultural Sciences, GKVK, Bengaluru 560 065, IN
2 School of Ecology and Conservation, University of Agricultural Sciences, GKVK, Bengaluru 560 065, IN
Source
Current Science, Vol 118, No 9 (2020), Pagination: 1448-1453Abstract
Endophytic fungi occur ubiquitously in all plants. Though their origin and evolution is enigmatic, they are known to play an important role in plant growth and development. Here we explore the endophytic fungal diversity of a perennial cree ping vine, Ipomea pes-caprae (family Convolulaceae), occurring naturally in the coastal sand dunes of peninsular India. Of the ten endophytes isolated from the plant, Fusarium oxysporum (MH511104) was found to grow even at 2 M NaCl in potato dextrose agar medium. The fungus was able to successfully colonize and impart salinity tolerance to salt -sensitive paddy variety, IR-64. We discuss these results in the context of increasing global interest on endophytes as a possible alternative route to crop i mprovement.Keywords
Endophytic Fungi, Ipomea pes-caprae, Paddy, Salt Stress.Full Text
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