Refine your search
Collections
Co-Authors
Journals
Year
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z All
Ganapathy, Kavitha
- Impact of Ultraviolet-B Radiation on Growth and Biochemical Composition of Botryococcus braunii Kutz.
Abstract Views :228 |
PDF Views:68
Authors
Chidambaram Kurinjimalar
1,
Kavitha Ganapathy
1,
Rangaraja Thevanathan
1,
Govindaswamy Kulandaivelu
1,
Ramasamy Rengasamy
1
Affiliations
1 Centre for Advanced Studies in Botany, University of Madras, Guindy Campus, Chennai - 600 025, IN
1 Centre for Advanced Studies in Botany, University of Madras, Guindy Campus, Chennai - 600 025, IN
Source
Current Science, Vol 116, No 1 (2019), Pagination: 89-95Abstract
The present study examines the impact of ultraviolet- B (UV-B) radiation stress on commercially significant microalga for biofuel application. Experimental alga Botryococcus braunii was treated under different doses of artificially enhanced UV-B radiation. The organism was treated under high dose rates of 1 and 5 Wm–2 with altered time durations of 15, 30, 45 and 60 min. It showed large variations in the growth characteristics analysed. The rate of whole-cell photosynthetic oxygen evolution showed steep drop in high dose compared to low dose-treated cultures. As a result, level of photosynthetic pigment chlorophyll a content decreased drastically while carotenoid level invariably increased. Consequently, the level of primary metabolites such as total carbohydrate, protein and lipid was drastically reduced under high dose while marginal decrement was observed at lowest dose of UV-B radiation. Overall, the impact of UV-B radiation on B. braunii led to a drop in protective mechanisms with associated decline in growth and cellular imbalance at high intensity studied.Keywords
Botryococcus braunii, Chlorophyll A, Oxygen Evolution, Ultraviolet-B Radiation.Full Text
References
- Vimalabai, C. P. M. and Kulandaivelu, G., Effects of prolonged UV-B enhanced fluorescent radiation on some marine microalgae. Biol. Plant., 2002, 45(3), 389–394.
- Häder, D. P., Kumar, H. D., Smith, R. C. and Worrest, R. C., Effects of solar UV radiation on aquatic ecosystem and interactions with climate change. Photochem. Photobiol. Sci., 2007, 6, 267–285.
- Zhou, W., Yin, K., Yuan, X. and Ning, X., Comparison of the effects of short-term UVB radiation exposure on phytoplankton photosynthesis in the temperate Changjiang and subtropical Zhujiang estuaries of China. J. Oceanogr., 2009, 65(5), 627–638.
- Holzinger, A. and Lütz, C., Algae and UV irradiation: effects on ultrastructure and related metabolic functions. Micron, 2006, 37, 190–207.
- Coohill, T. P., Ultraviolet action spectra (280 to 380 nm) and solar effectiveness spectra for higher plants. J. Photochem. Photobiol., 1989, 50(4), 451–457.
- Frederick, J. E., Ultraviolet sunlight reaching the earth’s surface: a review of recent research. J. Photochem. Photobiol., 1993, 57, 175–178.
- Hollosy, F., Effects of ultraviolet radiation on plant cell. Micron, 2002, 33, 179–197.
- Hideg, E. and Vass, I., UV-B induced free radical production in plant leaves and isolated thylakoid membranes. Plant Sci., 1996, 115, 251–260.
- Laurion, I. and Roy, S., Growth and photoprotection in three dinoflagellates (including two strains of Alexandrium tamarense) and one diatom exposed to four weeks of natural and enhanced ultraviolet-B radiation. J. Phycol., 2009, 45, 16–33.
- Rastogi, R. P., Richa, Sinha, R. P., Singh, S. P. and Häder, D. P., Photoprotective compounds from marine organisms. J. Ind. Microbiol. Biotechnol., 2010, 37, 537–558.
- Caldwell, M. M., Robberecht, R., Nowak, R. S. and Billings, W. D., Differential photosynthetic inhibition by ultraviolet radiation in species from the arctic–alpine life zone. Arct. Alp. Res., 1982, 14, 195–202.
- Barnes, P. W., Flint, S. D. and Caldwell, M. M., Photosynthesis damage and protective pigments in plants from a latitudinal arctic/ alpine gradient exposed to supplemental UV-B radiation in the field. Arct. Alp. Res., 1987, 19, 21–27.
- Banerjee, A., Sharma, R., Chisti, Y. and Banerjee, U. C., Botryococcus braunii: a renewable source of hydrocarbons and other chemicals. Crit. Rev. Biotechnol., 2002, 22(3), 245–279.
- Metzger, P. and Largeau, C., Botryococcus braunii: a rich source for hydrocarbons and related ether lipids. Appl. Microbiol. Biotechnol., 2005, 66(5), 486–496.
- Ranga Rao, A., Ravishankar, G. and Sarada, G., Cultivation of green alga Botryococcus braunii in raceway, circular ponds under outdoor conditions and its growth, hydrocarbon production. Bioresour. Technol., 2012, 123, 528–533.
- Ranga Rao, A., Sarada, G. and Ravishankar, G., Enhancement of carotenoids in green alga Botyrocccus braunii in various autotrophic media under stress conditions. Int. J. Biomed. Pharm. Sci., 2010, 4(2), 87–92.
- Yamaguchi, K. et al., Lipid composition of a green alga Botryococcus braunii. Agric. Biol. Chem., 1987, 57, 493–498.
- Kumar, H. D., Effects of radiation on blue green algae. Ann. Bot., 1963, 27, 723–733.
- Levasseur, M., Thompson, P. A. and Harrison, P. J., Physiological acclimation of marine phytoplankton to different nitrogen sources. J. Phycol., 1993, 29, 587–595.
- Lichtenthaler, H. K., Chlorophyll’s and carotenoids: pigments of photosynthetic biomembranes. In Methods in Enzymology (ed. Packer, L.), Academic Press, New York, USA, 1987, pp. 350– 382.
- Dubois, M., Gilles, K. A., Hamilton, J. K., Rebers, P. A. and Smith, F., Colorimetric method for determination of sugars and related substances. Anal. Chem., 1956, 28, 350–356.
- Bradford, M. M., A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding. Anal. Biochem., 1976, 72, 248–254.
- Folch, J., Lees, M. and Sloane-Stanley, G. H., A simple method for the isolation and purification of total lipids from animal tissues. J. Biol. Chem., 1957, 226, 497–509.
- Ashokkumar, V. and Rengasamy, R., Mass culture of Botryococcus braunii Kutz. under open raceway pond for biofuel production. Bioresour. Technol., 2012, 104, 394–399.
- Dayananda, C., Kumudha, A., Sarada, R. and Ravishankar, G. A., Isolation, characterization and outdoor cultivation of green microalgae Botryococcus sp. Sci. Res. Essays, 2010, 5(17), 2497–2505.
- Dayananda, C., Sarada, R., Kumar, V. and Ravishankar, G., Isolation and characterization of hydrocarbon producing green alga Botryococcus braunii from Indian freshwater bodies. J. Biotechnol., 2007, 10(1), 78–91.
- Cullen, J. J. and Lesser, M. P., Inhibition of photosynthesis by ultraviolet radiation as a function of dose and dosage rate: results for a marine diatom. Mar. Biol., 1991, 111, 183–190.
- Latha Prabha, G. and Kulandaivelu, G., Induced UV-B resistance against photosynthesis damage by adaptive mutagenesis in Synechococcus PCC 7942. Plant Sci., 2002, 162, 663–669.
- Noorudeen, A. M. and Kulandaivelu, G., On the possible site of inhibition of photosynthetic electron transport by ultraviolet-B (UV-B) radiation. Physiol. Plant, 1982, 55, 161–166.
- Longhurst, A. R., Role of the marine biosphere in the global carbon cycle. Limnol. Oceanogr., 1991, 36(8), 1507–1526.
- Ranga Rao, A., Sarada, R. and Ravishankar, G. A., Influence of CO2 on growth and hydrocarbon production in Botryococcus braunii. J. Microbiol. Biotechnol., 2007, 17(3), 414–419.
- Thompson, B. E., Worrest, R. C. and Van Dyke, H., The growth response of estuarine diatom (Melosira nummuloides (Dilw.) Ag.) to UV-B (290–320 nm) radiation. Estuaries, 1980, 3, 69–72.
- Häder, D. P., Rhiel, E. and Wehrmeyer, W., Ecological consequences of photo-movement and photobleaching in the marine
- flagellate Cryptomonas maculate. FEMS Microbiol. Ecol., 1988, 53, 9–18.
- Havaux, M., Carotenoid oxidation products as stress signals in plants. Plant J., 2013, 79, 597–606.
- Wu, H., Gao, K., Villafane, V. E., Watanabe, T. and Helbling, E. W., Effects of solar UV radiation on morphology and photosynthesis of filamentous cyanobacterium Arthrospira platensis. Appl. Environ. Microbiol., 2005, 7(9), 5004–5013.
- Döhler, G., Effect of UV-B radiation on biomass production, pigmentation and protein content of marine diatoms. Z. Naturforch., 1984, 39, 634–638.
- Kulandaivelu, G. and Noorudeen, A. M., Comparative study of the action of ultraviolet-C and ultraviolet-B radiation in photosynthetic electron transport. Physiol. Plant., 1983, 58, 389–394.
- Rai, L. C. and Mallick, N., Algal responses to enhanced ultraviolet B radiation. Proc. Natl. Acad. Sci. USA, 1998, B64, 125–146.
- Villafañe, E. V., Sundbäck, K., Figueroa, F. L. and Helbling, E. W., Photosynthesis in the aquatic environment as affected by UVR. In UV Effects in Aquatic Organisms and Ecosystems (eds Helbling, W. and Zagarese, H.), The Royal Society of Chemistry, Cambridge, UK, 2003, pp. 357–397.
- Wong, C., Teoh, M. L., Phang, S. M., Lim, P. E. and Beardall, J., Interactive effects of temperature and UV radiation on photosynthesis of Chlorella strains from polar, temperate and tropical environments: differential impacts on damage and repair. PLoS ONE, 2015, 10(10), e0139469; doi:10.1371/journal.pone.0139469.