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Purohit, Hemant J.

Evaluation of Peptide-Based Approach for Estimation of Nse and S-100ββ towards the Development of a Cost-Effective Test for Prognosis of AIS Patients

Abstract Views :181 | PDF Views:66

Authors

Amit R. Nayak ¹, Neha H. Lande ¹, Anuja P. Kawle ¹, Dinesh P. Kabra ¹, Nitin H. Chandak ¹, Hemant J. Purohit ², Girdhar M. Taori ¹, Hatim F. Daginawala ¹, Rajpal S. Kashyap ¹

Affiliations
1 Biochemistry Research Centre, Central India Institute of Medical Sciences, Nagpur 440 020, IN
2 Environmental Genomics Unit, National Environmental Engineering Research Institute, Nehru Marg, Nagpur 440 020, IN

Source

Current Science, Vol 109, No 8 (2015), Pagination: 1466-1470

Abstract

Here we compare the in-house ELISA technique for estimation of neuron-specific enolase (NSE) and S-100ββ (glial-specific protein) peptides with a commercial kit in serum samples of acute ischaemic stroke (AIS) patients. Nineteen improved and four expired AIS patients were included for the study. NSE concentrations were significantly higher (P < 0.05) in expired as compared with improved AIS patients by both inhouse ELISA and commercial kit method. Estimation of S-100ββ by only in-house ELISA showed significantly high (P < 0.05) levels in expired AIS patients. Peptide-based estimation of NSE and S-100ββ may be used for prognosis of AIS patients.

Keywords

Acute Ischaemic Stroke, Glial-Specific Protein, Neurological Disease, Neuron-specific Enolase, Prognosis.

Full Text

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Arid Ecosystem: Future Option for Carbon Sinks Using Microbial Community Intelligence

Abstract Views :159 | PDF Views:42

Authors

Leena Agarwal ¹, Asifa Qureshi ¹, Vipin Chandra Kalia ², Atya Kapley ¹, Hemant J. Purohit ¹, R. N. Singh ³

Affiliations
1 Environmental Genomics Division, CSIR-National Environmental Engineering Research Institute, Nehru Marg, Nagpur 440 020, IN
2 Microbial Biotechnology and Genomics Division, CSIR-Institute of Genomics and Integrative Biology, Delhi University Campus, Mall Road, Delhi 110 007, IN
3 CSIR-National Geophysical Research Institute, Hyderabad 500 007, IN

Source

Current Science, Vol 106, No 10 (2014), Pagination: 1357-1363

Abstract

Desert, comprising one-third of the Earth's surface, was a synonym for 'no life' as it supports very less or no life due to nutritional stress and extreme weather. Microbial autotrophic biochemistry is the principal source of carbon in arid environment, but understanding of these processes in arid ecosystem is limited. Emerging molecular tools have identified associations of phototrophic and chemolithoautotrophic communities often termed as 'biological soil crust' or 'microbiotic crust'. They are the sole sources of carbon and nitrogen, collectively providing soil stability to support vegetation. Here the curiosity arises, whether this phenomenon could be exploited in deserts for carbon sink using microbial community intelligence. By following the precipitation event under regulated nutrient supply that promotes the soil microbial intelligence for autotrophy would enrich soil carbon and nitrogen which in turn support plant growth in desert. Additionally, bioaugmentation of rhizobacteria could enhance the process. This will enable us to refine and formulate our strategies to exploit CO₂-fixing microorganisms in such niches vis-a-vis supporting the carbon sink using microbial community intelligence.

Keywords

Arid Ecosystem, Biological Soil Crust, Carbon Sequestration, Metagenome, Microbial Intelligence.

Full Text

Drug Discovery:Mining Microbes for Bioactive Compounds

Abstract Views :209 | PDF Views:28

Authors

Hemant J. Purohit ¹, Yogesh Shouche ², Arun Balakrishnan ³

Affiliations
1 National Environmental Engineering Research Institute, CSIR, Nehru Marg, Nagpur 440 020, IN
2 National Centre for Microbial Resource, Pune 411 021, IN
3 Omni Active Health Technologies, Mumbai 400 013, IN

Source

Current Science, Vol 113, No 12 (2017), Pagination: 2234-2236

Abstract

A collaborative programme involving industry and academia was initiated in September 2007 by the Department of Biotechnology to screen the bacterial diversity from various ecological niches in the country to search for novel bioactive molecules for therapeutic applications. The programme was designed to collect resource samples from the less-explored ecological niches at regular intervals for three years, to ensure a steady supply of microbes for high-throughput screening (HTS). The project had two major components. The first was targeted to generate a repository of 250,000 bacteria in three years by the academic research groups. The other component was preparation of microbial extracts and their screening on HTS platforms in four therapeutic areas followed by chemical profiling of potent extracts. Figure 1 shows the overall project plan.

Full Text

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Gut:A Key Mediating Centre for the Ageing Process

Abstract Views :205 | PDF Views:30

Authors

Hemant J. Purohit ¹, Jayant Deopujari ²

Affiliations
1 Environmental Genomics Division, National Environmental Engineering Research Institute, Nagpur 440 020, IN
2 Shrinidhi Ayurved Panchakarma Chikitsalay, 80 P&T Colony, Pratap Nagar, Nagpur 440 022, IN

Source

Current Science, Vol 114, No 11 (2018), Pagination: 2231-2233

Abstract

Survival was the main issue in the initial stage of evolution for humans. Now the priorities have shifted to immortality or graceful ageing. There is always a quest to understand the cognitive ageing process, which could be associated to a source of nectar or an organ that might be holding the key to ageing. Different doctrines evolved in different times or in parallel; they all explored and tried to understand in their own way the process of ageing. In Ayurveda ageing is defined as jara; and there are reports about manageing the ageing process, suggesting how to decelerate the jara process.

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