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Thakuria, Dwipendra
- Isolation, Characterization and Practical Significance of Cellulose Degrading Bacteria from the Gut Wall of Two Ecologically Distinct Earthworms
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Authors
Affiliations
1 Department of Plant and Environmental Protection Sciences, University of Hawaii, 3190 Maile Way, Honolulu, HI, 96822, US
2 Division of Life Sciences, Institute of Advanced Study in Science and Technology, DST (GoI), Paschim Boragaon, Guwahati 781 035, IN
3 School of Natural Resource Management, College of Post Graduate Studies, Central Agricultural University (Imphal), Umiam 793 103, IN
1 Department of Plant and Environmental Protection Sciences, University of Hawaii, 3190 Maile Way, Honolulu, HI, 96822, US
2 Division of Life Sciences, Institute of Advanced Study in Science and Technology, DST (GoI), Paschim Boragaon, Guwahati 781 035, IN
3 School of Natural Resource Management, College of Post Graduate Studies, Central Agricultural University (Imphal), Umiam 793 103, IN
Source
Current Science, Vol 114, No 07 (2018), Pagination: 1474-1484Abstract
This study has determined the relationship of cultur-able community of cellulose degrading bacteria (CDB) within the gut walls of two habitat-specific earthworm species, epigeic (compost heap inhabitant) earthworm, Perionyx excavatus (PE) and an endogeic (submerged rice field inhabitant), Glyphidrilus spelaeotes (GS) and their functional significance. The 16Sr RNA analysis for the isolated CDB from two ecologically distinct earthworms clearly showed the presence of distinct communities of CDB in their gut ecosystem. Enzymatic assay of cellulase for the isolated CDB showed sig-nificantly higher cellulase activity compared to the reference strain M-23, Cellulomonas cellulans (P < 0.01, one-way ANOVA). The functional signifi-cance of such high cellulase activity was also demon-strated by the enhancement of decomposition of rice straw and fresh vegetation biomass in the presence of native microbiota community. The growth rate of CDB of epigeic PE was approximately twice slower than that of CDBs of endogeic GS. The CDB of PE exhibited 12 polymorphs of esterase isozyme as against 4 polymorphs for CDB of GS. The present study emphasizes the functionally significant relation-ship of gut wall CDB and host earthworm for sustaining efficient C-cycling in agroecosystems.Keywords
Cellulase Activity, Easterase Isozyme, Earthworm–Microorganism Interactions, Glyphidrilus spelaeotes, Intestinal Microbiology, Soil Fauna.Full Text
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