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Gao, Chang-Yong
- Enhanced Oil Accumulation in Tobacco (Nicotiana tabacum L.) Leaves by Ectopic Overexpression of VgDGAT1a for Renewable Production of Biofuels
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Authors
Affiliations
1 Institute of Molecular Agriculture and Bioenergy, Shanxi Agricultural University, Taigu, Shanxi 030801, CN
2 Department of Life Science, Heze University, Heze, Shandong 274015, CN
1 Institute of Molecular Agriculture and Bioenergy, Shanxi Agricultural University, Taigu, Shanxi 030801, CN
2 Department of Life Science, Heze University, Heze, Shandong 274015, CN
Source
Current Science, Vol 114, No 06 (2018), Pagination: 1234-1240Abstract
To increase oil accumulation in the high-biomass vegetative organs of tobacco (Nicotiana tabacum L.) plants for renewable production of biofuels, VgDGAT1a isolated from developing seeds of Vernonia galamensis L. was ectopically overexpressed in tobacco leaves using a constitutive promoter. The transgenic tobacco leaves showed a 3.5–5.0-fold increase in oil content compared to the control, with a maximum increase of 9.2% (DW). The transgenic leaves also showed a substantial change in fatty acid composition, with significant enhancement of linoleic acid (18 : 2) and notable reduction of -linolenic acid (18 : 3). The overexpression of VgDGAT1a exhibited no deleterious effect on other phenotypes in the tobacco plant. These results will facilitate development of a novel system for lipid metabolic engineering in vegetative organs of plants, as well as provide a platform for the production of biofuels using the vegetative organs of commercial non-food crops.Keywords
Biofuels, Metabolic Engineering, Plant Oil, Tobacco Leaves, VgDGAT1a Gene.Full Text
References
- Barthole, G., Lepiniec, L., Rogowsky, P. M. and Baud, S., Controlling lipid accumulation in cereal grains. Plant Sci.: Int. J. Exp. Plant Biol., 2012, 185–186(4), 33–39.
- Ohlrogge, J. B. and Jaworski, J. G., Regulation of fatty acid synthesis. Annu. Rev. Plant Physiol. Plant Mol. Biol., 1997, 4, 109–136.
- Baud, S. and Lepiniec, L., Physiological and developmental regulation of seed oil production. Prog. Lipid Res., 2010, 3, 235–249.
- Guschina, I. A., Everard, J. D., Kinney, A. J., Quant, P. A. and Harwood, J. L., Studies on the regulation of lipid biosynthesis in plants: application of control analysis to soybean. Biochim. Biophys. Acta, 2014, 6, 1488–1500.
- Tajima, D. et al., Wrinkled 1 (WRI1) homologs, AP2-type transcription factors involving master regulation of seed storage oil synthesis in castor bean (Ricinus communis L.). Am. J. Plant Sci., 2013, 2, 333–339.
- Wu, X.-L., Liu, Z.-H., Hu, Z.-H. and Huang, R.-Z., BnWRI1 coordinates fatty acid biosynthesis and photosynthesis pathways during oil accumulation in rapeseed. J. Int. Plant Biol., 2014, 6, 582–593.
- Li, R., Yu, K., Hatanaka, T. and Hildebrand, D. F., Vernonia DGATs increase accumulation of epoxy fatty acids in oil. Plant Biotechnol. J., 2010, 2, 184–195.
- Yu, K., Li, R., Hatanaka, T. and Hildebrand, D., Cloning and functional analysis of two type 1 diacylglycerol acyltransferases from Vernonia galamensis. Phytochemistry, 2008, 5, 1119–1127.
- Li, R., Yu, K. and Hildebrand, D. F., DGAT1, DGAT2 and PDAT expression in seeds and other tissues of epoxy and hydroxy fatty acid accumulating plants. Lipids, 2010, 45, 145–157.
- Zhang, F.-Y., Yang, M.-F. and Xu, Y.-N., Silencing of DGAT1 in tobacco causes a reduction in seed oil content. Plant Sci., 2005, 4, 689–694.
- Jako, C. et al., Seed-specific overexpression of an Arabidopsis cDNA encoding a diacylglycerol acyltransferase enhances seed oil content and seed weight. Plant Physiol. Biochem.: PPB/Soc. Fran. Phys. Veg., 2001, 126, 861–874.
- Weselake, R. J. et al., Metabolic control analysis is helpful for informed genetic manipulation of oilseed rape (Brassica napus) to increase seed oil content. J. Exp. Bot., 2008, 59, 3543–3549.
- Shao, S. and Hegde, R. S., Membrane protein insertion at the endoplasmic reticulum. Annu. Rev. Cell. Dev. Biol., 2011, 27, 25–56.
- Cramer, C. L. et al., Bioproduction of human enzymes in transgenic tobacco. Ann. N.Y. Acad. Sci., 1996, 25, 62–71.
- Voinnet, O., Rivas, S., Mestre, P. and Baulcombe, D., An enhanced transient expression system in plants based on suppression of gene silencing by the p19 protein of tomato bushy stunt virus. Plant J.: Cell Mol. Biol., 2003, 5, 949–956.
- Youa, C.-X. et al., Chemical constituents and biological activities of the Purple Perilla essential oil against Lasioderma serricorne. Ind. Crops Prod., 2014, 61, 331–337.
- Grayburn, W. S., Collins, G. B. and Hildebrand, D. F., Fatty acid alteration by a Δ9 desaturase in transgenic tobacco tissue. Bio/Technol., 1992, 6, 675–678.
- Bouvier-Nave, P., Benveniste, P., Oelkers, P., Sturley, S. L. and Schaller, H., Expression in yeast and tobacco of plant cDNAs encoding acyl CoA: diacylglycerol acyltransferase. Eur. J. Bio-chem., 2000, 1, 85–96.
- Vanhercke, T., El Tahchy, A., Shrestha, P., Zhou, X. R., Singh, S. P. and Petrie, J. R., Synergistic effect of WRI1 and DGAT1 coexpression on triacylglycerol biosynthesis in plants. FEBS Lett., 2013, 4, 364–369.
- Livak, K. J. and Schmittgen, T. D., Analysis of relative gene expression data using real-time quantitative PCR and the 2–ΔΔCT method. Methods, 2001, 4, 402–408.
- Dahmer, M. L., Fleming, P. D., Collins, G. B. and Hildebrand, D. F., A rapid screening technique for determining the lipid composition of soybean seeds. J. Am. Oil Chem. Soc., 1989, 4, 543–548.
- Andrianov, V. et al., Tobacco as a production platform for biofuel: overexpression of Arabidopsis DGAT and LEC2 genes increases accumulation and shifts the composition of lipids in green biomass. Plant Biotechnol. J., 2010, 3, 277–287.
- Hanying, W., Chao, L., Minchun, L., Mingming, Z., Dan, G. and Yinong, X., Effects of monogalactoglycerolipid deficiency and diacylglycerol acyltransferase overexpression on oil accumulation in transgenic tobacco. Plant Mol. Biol. Rep., 2013, 5, 1077–1088.
- Li, R., Yua, K., Wu, Y., Tateno, M., Hatanaka, T. and Hildebrand, D. F., Vernonia DGATs can complement the disrupted oil and protein metabolism in epoxygenase-expressing soybean seeds. Metab. Eng., 2012, 1, 29–38.
- Lardizabal, K. et al., Expression of Umbelopsis ramanniana DGAT2A in seed increases oil in soybean. Plant Physiol., 2008, 148, 89–96.
- Sharma, N. et al., Transgenic increases in seed oil content are associated with the differential expression of novel Brassica-specific transcripts. BMC Genomics, 2008, 9, 619.
- Jako, C. et al., Seed-specific over-expression of an Arabidopsis cDNA encoding a diacylglycerol acyltransferase enhances seed oil content and seed weight. Plant Physiol. Biochem., 2001, 126, 861–887.
- Wen, O.-Y., Jianxiong, M. and Jingkai, D., Analytic study on the evaporable substances and fatty acids in aromatic (oriental) tobacco seeds. Acta Tabacaria Sin., 1996, 2, 51–55.
- Slocombe, S. P. et al., Oil accumulation in leaves directed by modification of fatty acid breakdown and lipid synthesis pathways. Plant Biotechnol. J., 2009, 7, 694–703.
- Gao, C.-Y., Mao, X., Shang, H.-Q., Ji, X.-J. and Li, R.-Z., Ectopic overexpression of MucACP-Δ9 desaturase leads to ω-7 fatty acid accumulation in tobacco leaves. Emir. J. Food Agric., 2016, 12, 890–896.