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Lavanya, B.
- Isolation and Characterization of NBS-Encoding Disease Resistance Gene Analogs in Watermelon against Fusarium Wilt
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Authors
Affiliations
1 Division of Biotechnology, Indian Institute of Horticultural Research, Bengaluru 560 089, IN
2 Division of Vegetable Crops, Indian Institute of Horticultural Research, Bengaluru 560 089, IN
1 Division of Biotechnology, Indian Institute of Horticultural Research, Bengaluru 560 089, IN
2 Division of Vegetable Crops, Indian Institute of Horticultural Research, Bengaluru 560 089, IN
Source
Current Science, Vol 117, No 4 (2019), Pagination: 617-626Abstract
Fusarium wilt (Fusarium oxysporum f. sp. niveum) in watermelon is one of the deadliest diseases around the globe, and availability of disease-resistant varieties is moderate. Disease management utilizing resistance genes (R-genes)/resistance gene analogs (RGAs) has proven to be a promising and successful approach. In the present study, six watermelon RGAs were isolated from wild, fusarium wilt resistant genotype IIHR-82 (Citrullus lanatus var. citroides) using degenerate primers that identify nucleotide binding site-leucine-rich repeat (NBS–LRR) regions. Multiple sequence alignment of these RGAs identified the characteristic NBS– LRR motif, and BLASTp search revealed similarity of these RGAs with other pathogenesis-related proteins. Phylogeny and motif analysis revealed genetic diversity of RGAs within those isolated from watermelon and with other plant R-genes. The watermelon RGAs isolated in this study contained both TIR–NBS–LRR (TNL) and non-TIR–NBS–LRR (CNL) classes of Rgenes. Protein secondary structure prediction of these watermelon RGAs revealed the composition of proteins, including α -helix, β -strand, disordered region and other template-related information. Watermelon RGAs identified in the present study will help in the development of RGA-based markers for resistance to fusarium wilt of watermelon.Keywords
Disease Management, Fusarium Wilt, Resistance Genes, Watermelon.Full Text
References
- FAO, Food and Agriculture Organization of the United Nations – Statistics Division, 2016; http://www.fao.org/faostat/en/#data/QC (accessed on 23 March 2018).
- Martyn, R. D. and Netzer, D., Resistance to races 0, 1 and 2 of Fusarium wilt of watermelon in Citrullus sp. PI-296341-FR. HortScience, 1991, 26(4), 429–432.
- Gusmini, G., Song, R. and Wehner, T. C., New sources of resistance to gummy stem blight in watermelon. Crop Sci., 2005, 45(2), 582–588.
- Thies, J. A. and Levi, A., Resistance of watermelon germplasm to the peanut ischolar_main-knot nematode. HortScience, 2003, 38(7), 1417– 1421.
- Thies, J. A. and Levi, A., Characterization of watermelon (Citrullus lanatus var. citroides) germplasm for resistance to ischolar_main-knot nematodes. HortScience, 2007, 42(7), 1530–1533.
- Thomas, C. E., Levi, A. and Caniglia, E., Evaluation of US plant introductions of watermelon for resistance to powdery mildew. HortScience, 2005, 40(1), 154–156.
- Davis, A. R., Levi, A., Wehner, T. and Pitrat, M., PI 525088PMR, a melon race 1 powdery mildew-resistant watermelon line. HortScience, 2006, 41(7), 1527–1528.
- Hopkins, D. L. and Thompson, C. M., Evaluation of Citrullus sp. germ plasm for resistance to Acidovorax avenae subsp. citrulli. Plant Dis., 2002, 86, 61–64.
- Sowell Jr, G., Rhodes, B. B. and Norton, J. D., New sources of resistance to watermelon anthracnose. J. Am. Soc. Hortic. Sci., 1980, 105, 197–199.
- Provvidenti, R., Inheritance of resistance to the Florida strain of zucchini yellow mosaic virus in watermelon. HortScience, 1991, 26(4), 407–408.
- Harris, K. R., Ling, K. S., Wechter, W. P. and Levi, A., Identification and utility of markers linked to the zucchini yellow mosaic virus resistance gene in watermelon. J. Am. Soc. Hortic. Sci., 2009, 134(5), 529–534.
- Swamy, K. M. et al., Development and evaluation of an interspecific population involving Citrullus lanatus var. citroides to develop pre-bred lines for resistance to WBNV in watermelon. In VIROCON, Indian Institute of Horticultural Research, Bengaluru, 2016.
- Guner, N. and Wehner, T. C., Overview of potyvirus resistance in watermelon. In Proceedings of the IX EUCARPIA Meeting Cucurbitaceae, l’institut National de la Recherche Agronom-ique, Avignon, France, 2008.
- Levi, A., Wechter, P., Massey, L., Carter, L. and Hopkins, D., An extended genetic linkage map for watermelon based on a testcross and a BC2F2 population. Am. J. Plant Sci., 2011, 2(2), 93.
- Levi, A. et al., High frequency oligonucleotides: targeting active gene (HFO-TAG) markers revealed wide genetic diversity among Citrullus spp. accessions useful for enhancing disease or pest resistance in watermelon cultivars. Genet. Resour. Crop Evol., 2013, 60(2), 427–440.
- Egel, D. S. and Martyn, R. D., Fusarium wilt of watermelon and other cucurbits. The Plant Health Instructor, 2007; doi:10.1094/ PHI-I-2007-0122-01 (updated 2013).
- Wan, H., Yuan, W., Bo, K., Shen, J., Pang, X. and Chen, J., Genome-wide analysis of NBS-encoding disease resistance genes in Cucumis sativus and phylogenetic study of NBS-encoding genes in Cucurbitaceae crops. BMC Genomics, 2013, 14(1), 109.
- Inohara, N., Chamaillard, M., McDonald, C. and Nunez, G., NOD–LRR proteins: role in host–microbial interactions and inflammatory disease. Ann. Rev. Biochem., 2005, 74, 355–383.
- DeYoung, B. J. and Innes, R. W., Plant NBS-LRR proteins in pathogen sensing and host defense. Nature Immunol., 2006, 7, 1243–1249.
- Martin, G. B., Bogdanove, A. J. and Sessa, G., Understanding the function of plant disease resistance proteins. Annu. Rev. Plant Biol., 2003, 54, 23–61.
- Kobe, B. and Kajava, A. V., The leucine-rich repeat as a protein recognition motif. Curr. Opin. Struct. Biol., 2001, 11(6), 725– 732.
- Ellis, J. G., Lawrence, G. J., Luck, J. E. and Dodds, P. N., Identification of regions in alleles of the flax rust resistance gene L that determine differences in gene-for-gene specificity. Plant Cell, 1999, 11, 495–506.
- Luck, J. E., Lawrence, G. J., Dodds, P. N., Shepherd, K. W. and Ellis, J. G., Regions outside of the leucine-rich repeats of flax rust resistance proteins play a role in specificity determination. Plant Cell, 2000, 12, 1367–1377.
- Meyers, B. C., Dickerman, A. W., Michelmore, R. W., Sivaramakrishnan, S., Sobral, B. W. and Young, N. D., Plant disease resistance genes encode members of an ancient and diverse protein family within the nucleotide‐binding superfamily. Plant J., 1999, 20(3), 317–332.
- Sekhwal, M. K., Li, P., Lam, I., Wang, X., Cloutier, S. and You, F. M., Disease resistance gene analogs (RGAs) in plants. Int. J. Mol. Sci., 2015, 16(8), 19248–19290.
- Harris, K. R., Wechter, W. P. and Levi, A., Isolation, sequence analysis, and linkage mapping of nucleotide binding site–leucinerich repeat disease resistance gene analogs in watermelon. J. Am. Soc. Hortic. Sci., 2009, 134(6), 649–657.
- Lin, X., Zhang, Y., Kuang, H. and Chen, J., Frequent loss of lineages and deficient duplications accounted for low copy number of disease resistance genes in Cucurbitaceae. BMC Genomics, 2013, 14, 335.
- Ori, N. et al., The I2C family from the wilt disease resistance locus I2 belongs to the nucleotide binding, leucine-rich repeat superfamily of plant resistance genes. Plant Cell, 1997, 9(4), 521– 532.
- Simons, G. et al., Dissection of the Fusarium I2 gene cluster in tomato reveals six homologs and one active gene copy. Plant Cell, 1998, 10(6), 1055–1068.
- McGrath, D. J., Gillespie, D. and Vawdrey, L., Inheritance of resistance to Fusarium oxysporum f. sp. lycopersici races 2 and 3 in Lycopersicon pennellii. Aust. J. Agric. Res., 1997, 38, 729–733.
- Scott, J. W. and Jones, J. P., Monogenic resistance in tomato to Fusarium oxysporum f. sp. lycopersici race 3. Euphytica, 1989, 40, 49–53.
- Catanzariti, A. M., Lim, G. T. and Jones, D. A., The tomato I‐3 gene: a novel gene for resistance to Fusarium wilt disease. New Phytol., 2015, 207(1), 106–118.
- Diener, A. C. and Ausubel, F. M., Resistance to Fusarium oxysporum 1, a dominant Arabidopsis disease-resistance gene, is not race specific. Genetics, 2005, 171(1), 305–321.
- Wechter, W. P., Kousik, C., McMillan, M. and Levi, A., Identification of resistance to Fusarium oxysporum f. sp. niveum race 2 in Citrullus lanatus var. citroides plant introductions. HortScience, 2012, 47(3), 334–338.
- Murray, M. G. and Thompson, W. R., Rapid isolation of high molecular weight plant DNA. Nucl. Acids Res., 1980, 8, 4321– 4325.
- Reddy, A. C., Venkat, S., Singh, T. H., Aswath, C., Reddy, K. M. and Reddy, D. L., Isolation, characterization and evolution of NBS–LRR encoding disease-resistance gene analogs in eggplant against bacterial wilt. Eur. J. Plant Pathol., 2015, 143(3), 417– 426.
- Bailey, T. L. et al., MEME SUITE: tools for motif discovery and searching. Nucleic Acids Res., 2009, 37, W202–W208.
- Kumar, S., Stecher, G. and Tamura, K., MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol. Biol. Evol., 2016, 33(7), 1870–1874.
- Kelley, L. A., Mezulis, S., Yates, C. M., Wass, M. N. and Sternberg, M. J., The Phyre2 web portal for protein modeling, prediction and analysis. Nature Proto., 2015, 10(6), 845–858.
- Koonin, E. V., Wolf, Y. I. and Karev, G. P., The structure of the protein universe and genome evolution. Nature, 2002, 420(6912), 218.
- Shao, M., Wang, S. and Wang, C., Incorporating ab initio energy into threading approaches for protein structure prediction. BMC Bioinfor. (Suppl 1), 2011, 12, S54.
- Zviling, M., Kochva, U. and Arkin, I. T., How important are transmembrane helices of bitopic membrane proteins? Biochim. Biophys. Acta (BBA)-Biomembr., 2007, 1768(3), 387–392.
- Bryan, G. T. et al., A single amino acid difference distinguishes resistant and susceptible alleles of the rice blast resistance gene Pi-ta. Plant Cell, 2000, 12(11), 2033–2045.
- Okuyama, Y. et al., A multifaceted genomics approach allows the isolation of the rice Pia‐blast resistance gene consisting of two adjacent NBS–LRR protein genes. Plant J., 2011, 66(3), 467– 479.
- Tan, X. et al., Global expression analysis of nucleotide binding site–leucine-rich repeat-encoding and related genes in Arabidopsis. BMC Plant Biol., 2007, 7(1), 56.
- Borhan, M. H., Gunn, N., Cooper, A., Gulden, S., Tör, M., Rimmer, S. R. and Holub, E. B., WRR4 encodes a TIR–NB–LRR protein that confers broad-spectrum white rust resistance in Arabidopsis thaliana to four physiological races of Albugo candida. Mol. Plant–Microb. Interact., 2008, 21(6), 757–768.
- Zhang, C. et al., The Ph-3 gene from Solanum pimpinellifolium encodes CC–NBS–LRR protein conferring resistance to Phytophthora infestans. Theoret. Appl. Genet., 2014, 127(6), 1353– 1364.
- Daryono, B. S., Wakui, K. and Natsuaki, K. T., Linkage analysis and mapping of SCAR markers linked to CMV-B2 resistance gene in melon. SABRAO J. Breed. Genet., 2010, 42(1), 35–45.
- Wan, H. et al., Analysis of TIR- and non-TIR–NBS–LRR disease resistance gene analogous in pepper: characterization, genetic variation, functional divergence and expression patterns. BMC Genomics, 2012, 13(1), 502.
- Naresh, P., Reddy, M. K., Reddy, A. C., Lavanya, B., Reddy, D. L. and Reddy, K. M., Isolation, characterization and genetic diversity of NBS–LRR class disease-resistant gene analogs in multiple virus resistant line of chilli (Capsicum annuum L.). 3 Biotech, 2017, 7(2), 114.
- Guo, S. et al., The draft genome of watermelon (Citrullus lanatus) and resequencing of 20 diverse accessions. Nature Genet., 2013, 45(1), 51.
- Lambel, S. et al., A major QTL associated with Fusarium oxysporum race 1 resistance identified in genetic populations derived from closely related watermelon lines using selective genotyping and genotyping-by-sequencing for SNP discovery. Theoret. Appl. Genet., 2014, 127(10), 2105–2115.
- Joobeur, T., King, J. J., Nolin, S. J., Thomas, C. E. and Dean, R. A., The fusarium wilt resistance locus Fom-2 of melon contains a single resistance gene with complex features. Plant J., 2004, 39(3), 283–297.
- Tezuka, T., Waki, K., Yashiro, K., Kuzuya, M., Ishikawa, T., Takatsu, Y. and Miyagi, M., Construction of a linkage map and identification of DNA markers linked to Fom-1, a gene conferring resistance to Fusarium oxysporum f. sp. melonis race 2 in melon. Euphytica, 2009, 168(2), 177.
- Okubara, P. A., Keller, K. E., McClendon, M. T., Inglis, D. A., McPhee, K. E. and Coyne, C. J., Y15_999Fw, a dominant SCAR marker linked to the Fusarium wilt race 1 (Fw) resistance gene in pea. Pisum Genet., 2005, 37, 30–33.
- Donald, T. M., Pellerone, F., Adam-Blondon, A. F., Bouquet, A., Thomas, M. R. and Dry, I. B., Identification of resistance gene analogs linked to a powdery mildew resistance locus in grapevine. Theoret. Appl. Genet., 2002, 104, 610–618.
- Wan, H., Zhao, Z., Malik, A. A., Qian, C. and Chen, J., Identification and characterization of potential NBS-encoding resistance genes and induction kinetics of a putative candidate gene associated with downy mildew resistance in Cucumis. BMC Plant Biol., 2010, 10, 186.
- Joshi, R. K., Mohanty, S., Subudhi, E. and Nayak, S., Isolation and characterization of NBS–LRR-resistance gene candidates in turmeric (Curcuma longa cv. surama). Genet. Mol. Res., 2010, 9, 1796–1806.
- Aarts, N., Metz, M., Holub, E., Staskawicz, B. J., Daniels, M. J. and Parker, J. E., Different requirements for EDS1 and NDR1 by disease resistance genes define at least two R genemediated signaling pathways in Arabidopsis. Proc. Natl. Acad. Sci. USA, 1998, 95, 10306–10311.
- Speulman, E., Bouchez, D., Holub, E. B. and Beynon, J. L., Disease resistance gene homologs correlate with disease resistance loci of Arabidopsis thaliana. Plant J., 1998, 14(4), 467–474.
- Noir, S., Combes, M.-C., Anthony, F. and Lashermes, P., Origin, diversity, and evolution of NBS-type disease-resistance gene homologues in coffee trees (Coffea L.). Mol. Genet. Genom., 2001, 265, 654–662.
- Deng, Z. et al., Cloning and characterization of NBS–LRR class resistance gene candidate sequences in citrus. Theoret. Appl. Genet., 2000, 101, 814–822.
- Zhang, L., Chen, R., Zhang, J., Ouyang, B., Xiao, J., Li, H. and Ye, Z., Cloning and analysis of resistance gene analogs from pepper (Capsicum annuum L.). Sci. Agric. Sin., 2008, 41(1), 169– 175.
- Chen, X. M., Line, R. F. and Leung, H., Genome scanning for resistancegene analogs in rice, barley, and wheat by high-resolution electrophoresis. Theoret. Appl. Genet., 1998, 97(3), 345–355.