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Das, B.
- Molecular Characterization and Toxin-Typing of Clostridium difficile Isolates of Dogs and Pigs from Assam and Mizoram of North East India
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Authors
B. Das
1,
R. K. Sharma
1,
P. Borah
2,
S. Das
3,
L. M. Barkalita
2,
R. K. Mandakini Devi
4,
B. C. Baishya
5
Affiliations
1 Department of Microbiology, Assam Agricultural University, Khanapara, Guwahati 781 022, IN
2 Department of Animal Biotechnology, Assam Agricultural University, Khanapara, Guwahati 781 022, IN
3 Department of Biotechnology, Gauhati University, Guwahati 781 014, IN
4 Department of Microbiology, Central Agricultural University, Selesih, Aizwal, Mizoram 796 014, IN
5 Department of Veterinary Clinical Medicine, Assam Agricultural University, Khanapara, Guwahati 781 022, IN
1 Department of Microbiology, Assam Agricultural University, Khanapara, Guwahati 781 022, IN
2 Department of Animal Biotechnology, Assam Agricultural University, Khanapara, Guwahati 781 022, IN
3 Department of Biotechnology, Gauhati University, Guwahati 781 014, IN
4 Department of Microbiology, Central Agricultural University, Selesih, Aizwal, Mizoram 796 014, IN
5 Department of Veterinary Clinical Medicine, Assam Agricultural University, Khanapara, Guwahati 781 022, IN
Source
Current Science, Vol 113, No 06 (2017), Pagination: 1099-1106Abstract
Clostridium difficile with its virulence factors A and B toxins cause Pseudomembranous colitis. Bacterium was isolated from 57 dog and 41 pig diarrheic faecalsamples in cycloserinecefaxitin fructose agar media and molecular detection was done by amplification of gluD gene (755 bp). Variability of toxin genes in positive isolates was tested by multiplex PCR. Detection ofbinary toxin genes (cdtA and cdtB) was also done. Results showed 33.67% positivity with 18 and 15 fromdog and pig respectively, from which 10 and 5 were toxigenic and 11 pig isolates exhibited binary toxin. PCR-RFLP demonstrated toxinotype 0 in all A+B+ isolates.Keywords
Diarrhoea, NE India, Pseudomembranous Colitis, Toxin-Typing, Virulence.Full Text
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