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Rai, Ashutosh
- Expression Analysis of Droughtinduced Genes in Wild Tomato Line (Solanum habrochaites)
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PDF Views:88
Authors
Affiliations
1 Division of Crop Improvement, Indian Institute of Vegetable Research, Varanasi 221 305, IN
2 Department of Biochemistry, Banaras Hindu University, Varanasi 221 005, IN
1 Division of Crop Improvement, Indian Institute of Vegetable Research, Varanasi 221 305, IN
2 Department of Biochemistry, Banaras Hindu University, Varanasi 221 005, IN
Source
Current Science, Vol 107, No 3 (2014), Pagination: 496-502Abstract
Many plant genes are regulated in response to abiotic stresses such as drought, high salinity, heat and cold, and their gene products function in stress response and tolerance. The whole process of plant adaptation to these environmental stresses is controlled by orchestration of complex molecular networks. In the present study, eight genes showing significant difference of expression on exposure to artificial drought stress in tomato, were selected from the previously performed microarray experiment. Expression analysis of the genes was done semi-quantitatively as well as quantitatively under artificially imposed drought stress and the results were almost similar to those of microarray experiment. Tissue-specific analysis of the genes, performed on tolerant line, revealed fairly a similar pattern of expression in ischolar_main, stem and leaf with notable differences in flower, which experienced the least influence of drought. The results confirmed that SIPRP16, SICYP51-17, SIMCPI19 and SIGDSL20 were downregulated in both the lines with stronger downregulation in sensitive line. SIWRKY4 was downregulated in both the lines with more folds of downregulation in tolerant line. SIEFH12 and SISNF4-15 were upregulated in tolerant line. SlUSPA9 was upregulated in both the lines with relatively more folds of upregulation in sensitive line.Keywords
Abiotic Stress, Drought, Gene Expression, Tomato, Transcription Factors.Full Text
- Proline-Rich Proteins May Regulate Free Cellular Proline Levels during Drought Stress in Tomato
Abstract Views :201 |
PDF Views:69
Authors
Affiliations
1 Division of Crop Improvement, ICAR-Indian Institute of Vegetable Research, Varanasi 221 305, IN
1 Division of Crop Improvement, ICAR-Indian Institute of Vegetable Research, Varanasi 221 305, IN
Source
Current Science, Vol 114, No 04 (2018), Pagination: 915-920Abstract
Proline (Pro)-rich proteins (PRPs), initially identified as structural proteins of cell wall, have emerged as multifunctional plant proteins in recent past. Their vibrant role in plant development and environmental stress promoted us to study a SlPRP gene of tomato, which was significantly downregulated under drought stress in a microarray experiment performed in our laboratory. Promoter analysis of SlPRP revealed a number of stress-responsive protein-binding sites, confirming its expression in response to stress. Expression of SlPRP gene in different tissues of tomato, viz. ischolar_main, stem, leaf and flower was studied to analyse the gene expression pattern in response to drought stress. Further, we have correlated the expression of SlPRP gene with Pro levels of the respective plant tissues under drought stress. In anticipation, it has been observed that downregulation of SlPRP gene is coupled with simultaneous increase in cellular Pro concentration in all the tissues under drought stress, except the ischolar_mains. This could help preserve the available cellular proline to function as osmoprotectant during stress. The present results propose a hypothesis where PRPs may regulate free cellular proline levels during drought stress by regulating their own gene expression. Thus, it may be concluded that transcription of PRPs in plants is synchronized with the cellular Pro concentration under environmental stress in order to provide drought tolerance to plants.Keywords
Drought Stress, Gene Expression, Prolinerich Proteins, Tomato.Full Text
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