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Nandi, Ankita
- Screening of Autochthonous Intestinal Microbiota as Candidate Probiotics Isolated from Four Freshwater Teleosts
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Authors
Ankita Nandi
1,
Goutam Banerjee
1,
Suhas Kumar Dan
1,
Pinki Ghosh
1,
Koushik Ghosh
2,
Arun Kumar Ray
1
Affiliations
1 Fisheries Laboratory, Department of Zoology, Visva-Bharati University, Santiniketan 731 235, IN
2 Department of Zoology, University of Burdwan, Golap Bag, Burdwan 713 104, IN
1 Fisheries Laboratory, Department of Zoology, Visva-Bharati University, Santiniketan 731 235, IN
2 Department of Zoology, University of Burdwan, Golap Bag, Burdwan 713 104, IN
Source
Current Science, Vol 113, No 04 (2017), Pagination: 767-773Abstract
In this study altogether 109 autochthonous gut bacteria were screened from 4 fish species (Labeo rohita, Labeo bata, Catla catla and Puntius javanicus), of which 13 isolates displayed antagonism to 4 fish potent pathogens, namely Aeromonas salmonicida, Aeromonas hydrophila, Pseudomonas fluorescens and Aeromonas sobria. Eight promising isolates were further evaluated for extracellular enzyme production, non-hemolytic activity, bile tolerance and identified by 16S rRNA sequencing. Strains CCF7 (identified as Bacillus sp.), CCH9 and PJH1 (identified as two strains of Bacillus amyloliquefaciens) exhibited high score in antagonism assay and fulfilled other probiotic criteria, including safety aspects. However, application of these probiotics in aquaculture industries requires in vivo experiments and other information like immune modulating efficiency and binding ability on gut.Keywords
Antagonism, Bacillus Species, Fish Gut Bacteria, Athogen, Probiotics.Full Text
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