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Varma, Ajit
- Amelioration of Soybean Plant from Saline-Induced Condition by Exopolysaccharide Producing Pseudomonas-Mediated Expression of High Affinity K+-transporter (HKT1) Gene
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PDF Views:79
Authors
Affiliations
1 Department of Biological Sciences, College of Arts, Science and Humanities, Mody University of Science and Technology, Lakshmangarh, Sikar 332 311, IN
2 Amity Institute of Microbial Technology, Block ‘E-3’, 4th Floor, Amity University Campus, Sector-125, Noida 201 313, IN
1 Department of Biological Sciences, College of Arts, Science and Humanities, Mody University of Science and Technology, Lakshmangarh, Sikar 332 311, IN
2 Amity Institute of Microbial Technology, Block ‘E-3’, 4th Floor, Amity University Campus, Sector-125, Noida 201 313, IN
Source
Current Science, Vol 111, No 12 (2016), Pagination: 1961-1967Abstract
There is a consensus that soil salinization causes decreased agricultural production. Several plants may adapt to survive under high salt stress wherein glycophytes fail to grow. Among transporters in plants, the sodium transporter, also known as high-affinity K+-transporter (HKT1) that comes under the HKT gene family, is involved in uptake of sodium through the ischolar_mains and its recirculation from shoot to ischolar_main. In the present study, we have examined the role of transporter HKT1 in soybean plants upon addition of 200 mM NaCl and treatment with Pseudomonas sp. strain AK-1. It is reported that HKT1 is permeable to K+/Na+ and systemically alleviates salinity stress through upregulation of gene expression in shoots and down-regulation in ischolar_mains. Higher transcription levels in shoot recirculate Na+ from shoot xylem to ischolar_main phloem whereas lower transcription levels in ischolar_main do not allow sodium to enter the plants through ischolar_main cells. We have also examined role of exopolysaccharide (EPS) produced by strain AK-1 which helps in the binding of free Na+ from soil and thus makes Na+ unavailable to the soybean plants. Strain with EPS showed decrease in electrical conductivity of soil from 1.1 to 0.9 dS/m in the presence of 200 mM NaCl. In conclusion, treatment with Pseudomonas sp. strain AK-1 exhibits significant rise in shoot/ischolar_main length, number of lateral ischolar_mains, shoot/ischolar_main fresh weight and decreased Na+/K+ ratio under salinity stress.Keywords
Abiotic Stress, Exopolysaccharide, Plant Growth-Promoting Bacteria, Sodium Transporter, Soybean Plant.Full Text
- Comparative Expression Analysis of Defence-Related Genes in Bacillus-Treated Glycine Max upon Challenge Inoculation with Selective Fungal Phytopathogens
Abstract Views :246 |
PDF Views:71
Authors
Affiliations
1 Mandsaur University, Mandsaur 458 001, IN
2 Amity Institute of Microbial Technology, Amity University, Noida 201 303,, IN
1 Mandsaur University, Mandsaur 458 001, IN
2 Amity Institute of Microbial Technology, Amity University, Noida 201 303,, IN
Source
Current Science, Vol 115, No 10 (2018), Pagination: 1950-1956Abstract
Activation of defence-related genes by the application of beneficial bacteria leads to prior protection against pathogens through induced systemic resistance. The present study was carried out to examine the qRT– PCR-based relative quantification of differently expressed defence-related genes in soybean (Glycine max L. Merrill) plants primed with Bacillus sp. strain SJ-5 against the fungal pathogen Rhizoctonia solani and Fusarium oxysporum. In this context, molecular characterization of plant growth promoting and biocontrol genes of SJ-5 was done by PCR followed by homology analysis. In the GC-MS analysis of SJ-5 volatile organic compounds, potent antifungal compound bis(2-ethyl-hexyl) phthalate and antioxidant compound butylated hydroxy toluene were reported with the highest peak area 47.96% and 21.82% respectively, along with other antifungal compounds in small proportion. Qualitative expression of different defence-related genes like lipoxygenase, phenylalanine ammonia-lyase 2, peroxidase, polyphenol oxidase, endo-1,3-beta-glucanase, catalase, defensin-like protein, vegetative storage protein and chitinase class I was found elicited in the plants primed with SJ-5 against the fungal pathogens. In the qPCR analysis, the highest upregulation was observed in the transcript profile of ppojh2 in the treatments T5 and T6 with 4.12- and 4.06-fold increase respectively.Keywords
Defence-Related Genes, Induced Systemic Resistance, Plant Growth Promoting Bacteria, Volatile Organic Compounds.Full Text
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