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Deleterious Missense Single Nucleotide Polymorphism Alters the Structural Conformation of Human Anti-Apoptotic Bcl-2 Protein


Affiliations
1 Biochemistry and Toxicology Division, Department of Zoology, University of Calicut, Malappuram 673 635, India
 

Identification and understanding the mechanism behind tumorigenesis is crucial for developing new strategies in cancer treatments. In this in silico study, we used the 14 different tools to evaluate the conver-gent deleterious missense Bcl-2 SNPs. Out of 37 mis-sense single nucleotide polymorphism (SNPs), five are deleterious. Molecular modelling and structural and functional evaluation of the mutant proteins were carried out to understand their clinical significance. All deleterious missense SNPs alter the structural stability of the protein. Also H94P deleterious mis-sense SNPs alter the ligand-binding ability of Bcl-2. The results indicate that the mutant antiapoptotic Bcl-2 protein may contribute to tumorigenesis in different ways, depending upon the mutation location.

Keywords

Apoptosis, Anti-apoptotic Protein Bcl-2, Deleterious Single Nucleotide Polymorphism, In Silico Evalu-ation, Protein Modelling.
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  • Deleterious Missense Single Nucleotide Polymorphism Alters the Structural Conformation of Human Anti-Apoptotic Bcl-2 Protein

Abstract Views: 237  |  PDF Views: 74

Authors

P. P. Anand
Biochemistry and Toxicology Division, Department of Zoology, University of Calicut, Malappuram 673 635, India
Y. Shibu Vardhanan
Biochemistry and Toxicology Division, Department of Zoology, University of Calicut, Malappuram 673 635, India

Abstract


Identification and understanding the mechanism behind tumorigenesis is crucial for developing new strategies in cancer treatments. In this in silico study, we used the 14 different tools to evaluate the conver-gent deleterious missense Bcl-2 SNPs. Out of 37 mis-sense single nucleotide polymorphism (SNPs), five are deleterious. Molecular modelling and structural and functional evaluation of the mutant proteins were carried out to understand their clinical significance. All deleterious missense SNPs alter the structural stability of the protein. Also H94P deleterious mis-sense SNPs alter the ligand-binding ability of Bcl-2. The results indicate that the mutant antiapoptotic Bcl-2 protein may contribute to tumorigenesis in different ways, depending upon the mutation location.

Keywords


Apoptosis, Anti-apoptotic Protein Bcl-2, Deleterious Single Nucleotide Polymorphism, In Silico Evalu-ation, Protein Modelling.

References





DOI: https://doi.org/10.18520/cs%2Fv120%2Fi4%2F666-675