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Effective Use of Synthetic Seed Technology in the Regeneration of Cymbidium aloifolium using Protocorm-Like Bodies


Affiliations
1 Orchid Laboratory, Botany Department, Panjab University, Chandigarh 160 014, India
 

Synthetic seed technology offers tremendous potential in micropropagation. It deals with in vitro conservation and storage of rare and endangered plant species along with their easy handling and transportation. This technology is becoming prevelant due to its wide applications in germplasm conservation and for exchanges between countries in floricultural trade. The present study examines the regeneration and conversion capabilities of Cymbidium aloifolium using protocorm-like bodies when stored at different temperatures. The propagules showed high proliferative potential by multiplication and complete plantlets were obtained in 58 days on basal M medium supplemented with 1 mg l–1 of indole-3-acetic acid.

Keywords

Cymbidium aloifolium, Protocorm-Like Bodies, Regeneration, Synthetic Seeds.
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  • Effective Use of Synthetic Seed Technology in the Regeneration of Cymbidium aloifolium using Protocorm-Like Bodies

Abstract Views: 227  |  PDF Views: 68

Authors

Shivani Verma
Orchid Laboratory, Botany Department, Panjab University, Chandigarh 160 014, India
Promila Pathak
Orchid Laboratory, Botany Department, Panjab University, Chandigarh 160 014, India

Abstract


Synthetic seed technology offers tremendous potential in micropropagation. It deals with in vitro conservation and storage of rare and endangered plant species along with their easy handling and transportation. This technology is becoming prevelant due to its wide applications in germplasm conservation and for exchanges between countries in floricultural trade. The present study examines the regeneration and conversion capabilities of Cymbidium aloifolium using protocorm-like bodies when stored at different temperatures. The propagules showed high proliferative potential by multiplication and complete plantlets were obtained in 58 days on basal M medium supplemented with 1 mg l–1 of indole-3-acetic acid.

Keywords


Cymbidium aloifolium, Protocorm-Like Bodies, Regeneration, Synthetic Seeds.

References





DOI: https://doi.org/10.18520/cs%2Fv120%2Fi3%2F570-572