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Amelioration of Bleomycin-Induced Acute Lung Injury and Epithelial-Mesenchymal Transition by Baicalein in Mice


Affiliations
1 Department of Veterinary Pathology, and Animal Disease Research Centre, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana 141 004, India
2 Animal Disease Research Centre, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana 141 004, India
 

During lung fibrosis, acute pulmonary inflammation and epithelial–mesenchymal transition (EMT) play important roles. The present study analyses the ameliorative effect of baicalein, a bioactive flavonoid present in the dry ischolar_mains of Scutellaria baicalensis Georgi, on bleomycin-induced acute lung injury and subsequent EMT. Mice received a single intratracheal instillation of saline containing bleomycin @1 mg/kg body wt. Baicalein in different doses (0.1, 1.0, 10 mg/kg) was given intraperitoneally daily for one week. Pulmonary inflammation and EMT parameters were evaluated. Baicalein significantly attenuated bleomycin-induced pulmonary inflammatory and structural changes. Thus, treatment with baicalein ameliorates bleomycininduced acute inflammation and subsequent early stage of EMT.

Keywords

Acute Lung Injury, Baicalein, Bleomycin, Epithelial-Mesenchymal Transition, Mice.
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  • Amelioration of Bleomycin-Induced Acute Lung Injury and Epithelial-Mesenchymal Transition by Baicalein in Mice

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Authors

Dheeraj Kumar Sharma
Department of Veterinary Pathology, and Animal Disease Research Centre, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana 141 004, India
Nittin Dev Singh
Department of Veterinary Pathology, and Animal Disease Research Centre, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana 141 004, India
Geeta Devi Leishangthem
Animal Disease Research Centre, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana 141 004, India
Harmanjit Singh Banga
Department of Veterinary Pathology, and Animal Disease Research Centre, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana 141 004, India

Abstract


During lung fibrosis, acute pulmonary inflammation and epithelial–mesenchymal transition (EMT) play important roles. The present study analyses the ameliorative effect of baicalein, a bioactive flavonoid present in the dry ischolar_mains of Scutellaria baicalensis Georgi, on bleomycin-induced acute lung injury and subsequent EMT. Mice received a single intratracheal instillation of saline containing bleomycin @1 mg/kg body wt. Baicalein in different doses (0.1, 1.0, 10 mg/kg) was given intraperitoneally daily for one week. Pulmonary inflammation and EMT parameters were evaluated. Baicalein significantly attenuated bleomycin-induced pulmonary inflammatory and structural changes. Thus, treatment with baicalein ameliorates bleomycininduced acute inflammation and subsequent early stage of EMT.

Keywords


Acute Lung Injury, Baicalein, Bleomycin, Epithelial-Mesenchymal Transition, Mice.

References





DOI: https://doi.org/10.18520/cs%2Fv119%2Fi6%2F962-972