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Anti-Tumour and Immune Enhancing Activities of MLAA-22379–387 on Acute Myeloid Leukemia


Affiliations
1 Department of Pediatrics, Medical School of Xi’an Jiaotong University, Xi’an Xincheng Xiwu Road 157, Xi’an 710004, Shaanxi Province, China
2 Department of Hematology, Medical School of Xi’an Jiaotong University, Xi’an Xincheng Xiwu Road 157, Xi’an 710004, Shaanxi Province, China
 

We have earlier demonstrated that MLAA-22379–387is a novel, acute, monocytic, leukemia-associated antigen epitope in vitro. In this study, the effect and mechanism of MLAA-22379–387on animals have been further examined. We found that tumour weight and volume had significantly decreased in SCID-injected THP-1 mice with MLAA-22379–387treatment for two weeks. MLAA-22379–387induced cytotoxic T lymphocytes (CTL) activity in A549, MCF-7, THP-1, U937 and T2 cells, especially significant CTL activity at effector/target ratio of 50 : 1 in THP-1 cells. The percentage of CD3 + CD8 + T cells had significantly increased, while the percentage of CD4 + CD25 + T cells had significantly decreased in MLAA-22379–387treatment group compared to other groups. Levels of IL-2, IFN-γand IgG had significantly increased, but levels of TGF-βand IL-10 had significantly decreased after MLAA-22379–387vac-cination for two weeks. Thus, we may conclude that MLAA-22379–387treatment effectively improves the immune system, thus indicating tumouricidal capacity in leukaemic mice. These findings highlight the potential application of MLAA-22379–387 as an efficient target for immunotherapy in acute myeloid leukemia.

Keywords

Acute Myeloid Leukemia, Anti-Tumour Activity, Immunotherapy, Mice.
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  • Anti-Tumour and Immune Enhancing Activities of MLAA-22379–387 on Acute Myeloid Leukemia

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Authors

Jing Li
Department of Pediatrics, Medical School of Xi’an Jiaotong University, Xi’an Xincheng Xiwu Road 157, Xi’an 710004, Shaanxi Province, China
Wanggang Zhang
Department of Hematology, Medical School of Xi’an Jiaotong University, Xi’an Xincheng Xiwu Road 157, Xi’an 710004, Shaanxi Province, China
Ju Bai
Department of Hematology, Medical School of Xi’an Jiaotong University, Xi’an Xincheng Xiwu Road 157, Xi’an 710004, Shaanxi Province, China
Bo Zhong
Department of Pediatrics, Medical School of Xi’an Jiaotong University, Xi’an Xincheng Xiwu Road 157, Xi’an 710004, Shaanxi Province, China
Huiyuan Wang
Department of Pediatrics, Medical School of Xi’an Jiaotong University, Xi’an Xincheng Xiwu Road 157, Xi’an 710004, Shaanxi Province, China
Yan Geng
Department of Pediatrics, Medical School of Xi’an Jiaotong University, Xi’an Xincheng Xiwu Road 157, Xi’an 710004, Shaanxi Province, China
Qiaoyan Jin
Department of Pediatrics, Medical School of Xi’an Jiaotong University, Xi’an Xincheng Xiwu Road 157, Xi’an 710004, Shaanxi Province, China
Juanjuan Hao
Department of Pediatrics, Medical School of Xi’an Jiaotong University, Xi’an Xincheng Xiwu Road 157, Xi’an 710004, Shaanxi Province, China
Yang Zhang
Department of Pediatrics, Medical School of Xi’an Jiaotong University, Xi’an Xincheng Xiwu Road 157, Xi’an 710004, Shaanxi Province, China

Abstract


We have earlier demonstrated that MLAA-22379–387is a novel, acute, monocytic, leukemia-associated antigen epitope in vitro. In this study, the effect and mechanism of MLAA-22379–387on animals have been further examined. We found that tumour weight and volume had significantly decreased in SCID-injected THP-1 mice with MLAA-22379–387treatment for two weeks. MLAA-22379–387induced cytotoxic T lymphocytes (CTL) activity in A549, MCF-7, THP-1, U937 and T2 cells, especially significant CTL activity at effector/target ratio of 50 : 1 in THP-1 cells. The percentage of CD3 + CD8 + T cells had significantly increased, while the percentage of CD4 + CD25 + T cells had significantly decreased in MLAA-22379–387treatment group compared to other groups. Levels of IL-2, IFN-γand IgG had significantly increased, but levels of TGF-βand IL-10 had significantly decreased after MLAA-22379–387vac-cination for two weeks. Thus, we may conclude that MLAA-22379–387treatment effectively improves the immune system, thus indicating tumouricidal capacity in leukaemic mice. These findings highlight the potential application of MLAA-22379–387 as an efficient target for immunotherapy in acute myeloid leukemia.

Keywords


Acute Myeloid Leukemia, Anti-Tumour Activity, Immunotherapy, Mice.

References





DOI: https://doi.org/10.18520/cs%2Fv118%2Fi6%2F892-900