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Iron and Zinc Bioavailability from Madhukar × Swarna Derived Biofortifed Rice Lines


Affiliations
1 ICMR-National Institution of Nutrition, Indian Council of Medical Research, Hyderabad 500 007, India
2 ICAR-Indian Institute of Rice Research, Hyderabad 500 030, India
3 ICAR-Indian Institute of Millets Research, Hyderabad 500 030, India
 

Rice is the prime target of biofortification as it provides calories for about half of the world’s population. We assessed the iron (Fe) and zinc (Zn) bioavailability from polished rice grains of three high Fe and Zn recombinant inbred lines (RILs) using simulated in vitro digestion/Caco-2 cell model. Ferritin induction and 65Zn uptake were used as surrogate for Fe and Zn bioavailability respectively. Fe and Zn concentration in both unpolished and polished rice grains of three RILs was higher compared to Swarna, a parent and popular variety used as control. The grain Fe concentration was positively correlated (r = 0.94) to Zn concentration. There was a 2-fold induction of ferritin (42.4 ± 3.2 ng/mg protein) in Caco-2 cells only in the presence of ascorbic acid and 3-fold increase in 65Zn uptake (17.7 ± 2.4 pmol/mg protein) from the RIL 185M compared to Swarna (ferritin: 24.8 ± 4.0 ng/mg protein; 65Zn uptake: 5.8 ± 0.3 pmol/mg protein). Phytic acid was highest (8.75 mg/g) in 185 M but that did not affect bioavailability of Fe and Zn. Thus, improving the density of Fe and Zn in grains of conventionally bred rice lines has the potential to enhance the bioavailability of Fe and Zn.

Keywords

Ascorbic Acid, Caco-2 Cells, Ferritin Induction, Phytic Acid, Recombinant Inbred Lines.
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  • Iron and Zinc Bioavailability from Madhukar × Swarna Derived Biofortifed Rice Lines

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Authors

Raghu Pullakhandam
ICMR-National Institution of Nutrition, Indian Council of Medical Research, Hyderabad 500 007, India
Surekha Agarwal
ICAR-Indian Institute of Rice Research, Hyderabad 500 030, India
V. G. N. Tripura Venkata
ICAR-Indian Institute of Rice Research, Hyderabad 500 030, India
C. V. Ratnavathi
ICAR-Indian Institute of Millets Research, Hyderabad 500 030, India
K. Madhavan Nair
ICMR-National Institution of Nutrition, Indian Council of Medical Research, Hyderabad 500 007, India
Sarla Neelamraju
ICAR-Indian Institute of Rice Research, Hyderabad 500 030, India

Abstract


Rice is the prime target of biofortification as it provides calories for about half of the world’s population. We assessed the iron (Fe) and zinc (Zn) bioavailability from polished rice grains of three high Fe and Zn recombinant inbred lines (RILs) using simulated in vitro digestion/Caco-2 cell model. Ferritin induction and 65Zn uptake were used as surrogate for Fe and Zn bioavailability respectively. Fe and Zn concentration in both unpolished and polished rice grains of three RILs was higher compared to Swarna, a parent and popular variety used as control. The grain Fe concentration was positively correlated (r = 0.94) to Zn concentration. There was a 2-fold induction of ferritin (42.4 ± 3.2 ng/mg protein) in Caco-2 cells only in the presence of ascorbic acid and 3-fold increase in 65Zn uptake (17.7 ± 2.4 pmol/mg protein) from the RIL 185M compared to Swarna (ferritin: 24.8 ± 4.0 ng/mg protein; 65Zn uptake: 5.8 ± 0.3 pmol/mg protein). Phytic acid was highest (8.75 mg/g) in 185 M but that did not affect bioavailability of Fe and Zn. Thus, improving the density of Fe and Zn in grains of conventionally bred rice lines has the potential to enhance the bioavailability of Fe and Zn.

Keywords


Ascorbic Acid, Caco-2 Cells, Ferritin Induction, Phytic Acid, Recombinant Inbred Lines.

References





DOI: https://doi.org/10.18520/cs%2Fv118%2Fi3%2F455-461