Current Science

Open Access Open Access  Restricted Access Subscription Access

Transcriptomic Analysis of Chilling-treated Tobacco (Nicotiana tabacum) Leaves Reveals Chilling-Induced Lignin Biosynthetic Pathways


Affiliations
1 Key Laboratory of Tobacco Biology and Processing, Ministry of Agriculture, Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao 266101, China
2 Longyan Tobacco Agricultural Science Institute, Longyan, Fujian 364000,, China
 

Chilling stress is one of the most important environ-mental stresses for chilling-sensitive species. The pre-sent study conducted RNA-Seq and WGCNA analysis to clarify the correlation patterns among genes of dif-ferent treatments in tobacco (Nicotiana tabacum). A total of 10,355 DEGs were found in chilling treatment relative to control treatment. Additionally, functional annotations revealed that 48 genes were found to be specifically expressed in lignin biosynthesis pathway in tobacco seedlings under chilling stress. Our results revealed that the biosynthesis of caffeoyl-CoA was regulated by HCT and C3H. Furthermore, the G-type lignin biosynthesis branch was enhanced under low temperature, which contributed to an increase in lig-nin content and changes in lignin composition, indi-cating that G-type lignin may play an important role in tobacco’s resistance to chilling stress.

Keywords

Chilling Stress, Lignin Biosynthesis, Nicotiana Tabacum, Transcriptomic, WGCNA.
User
Notifications
Font Size

  • Croteau, R., Kutchan, T. M. and Lewis, N. G., Natural products (secondary metabolites). In Biochemistry and Molecular Biology of Plants (eds Buchanan, B., Gruissem, W. and Jones, R. L.), American Society of Plant Physiologists, Rockville, MD, USA, 2000, pp. 1250–1318.

  • Merali, Z., Mayer, M. J., Parker, M. L., Michael, A. J., Smith, A. C. and Waldron, K. W., Expression of a bacterial, phenyl-propanoid-metabolizing enzyme in tobacco reveals essential roles of phenolic precursors in normal leaf development and growth. Physiol. Plantarum., 2012, 145(2), 260–274.

  • Humphreys, J. M. and Chapple, C., Rewriting the lignin roadmap. Curr. Opin. Plant Biol., 2002, 5, 224–229.

  • Vanholme, R., Morreel, K., Ralph, J. and Boerjan, W., Lignin en-gineering. Curr. Opin. Plant Biol., 2008, 11(3), 278–285.

  • Ali, M. B. and McNear Jr, D. H., Induced transcriptional profiling of phenylpropanoid pathway genes increased flavonoid and lignin content in Arabidopsis leaves in response to microbial products. BMC Plant Biol., 2014, 14, 84.

  • Wen, P. F., Chen, J. Y., Wan, S. B., Kong, W. F., Zhang, P. and Wang, W., Salicylic acid activates phenylalanine ammonialyase in grape berry in response to high temperature stress. J. Plant Growth Regul., 2008, 55, 1–10.

  • Janska, A., Aprile, A., Zamecnik, J., Cattivelli, L. and Ovesna, J., Transcriptional responses of winter barley to cold indicate nucleo-some remodelling as a specific feature of crown tissues. Funct. In-tegr. Genomic., 2011, 11, 307–325.

  • Wei, H., Dhanaraj, A. L., Arora, R., Rowland, L. J., Fu, Y. and Sun, L., Identification of cold acclimation-responsive Rhododen-dron genes for lipid metabolism, membrane transport and lignin biosynthesis: Importance of moderately abundant ESTs in ge-nomic studies. Plant Cell Environ., 2006, 29, 558–570.

  • Khaledian, Y., Maali-Amiri, R. and Talei, A., Phenylpropanoid and antioxidant changes in chickpea plants during cold stress. Russ. J. Plant Physiol., 2015, 62(6), 772–778.

  • Moura, J. C. M. S., Bonine, C. A. V., De Oliveira Fernandes Vi-ana, J., Dornelas, M. C. and Mazzafera, P., Abiotic and biotic stresses and changes in the lignin content and composition in plants. J. Integr. Plant Biol., 2010, 52, 360–376.

  • Trapnell, C. et al., Transcript assembly and quantification by RNA-seq reveals unannotated transcripts and isoform switching during cell differentiation. Nat. Biotechnol., 2010, 28(5), 511–515.

  • Cao, W. H., Liu, J., He, X. J., Mu, R. L., Zhou, H. L., Chen, S. Y. and Zhang, J. S., Modulation of ethylene responses affects plant salt-stress responses. Plant Physiol., 2007, 143, 707–719.

  • Miedes, E., Vanholme, R., Boerjan, W. and Molina, A., The role of the secondary cell wall in plant resistance to pathogens. Front. Plant Sci., 2014, 5, 358.

  • Grabber, J. H., Ralph, J., Lapierre, C. and Barriere, Y., Genetic and molecular basis of grass cell-wall degradability, I. Lignin-cell wall matrix interactions. CR Biol., 2004, 327, 455–465.

  • Hausman, J. F., Evers, D., Thiellement, H. and Jouve, L., Compared responses of poplar cuttings and in vitro raised shoots to short-term chilling treatments. Plant Cell Rep., 2000, 19, 954–960.

  • Gindl, W., Grabner, M. and Wimmer, R., The influence of tem-perature on latewood lignin content in treeline Norway spruce compared with maximum density and ring width. Trees-Struct. Funct., 2000, 14, 409–414.

  • Weng, J. K. and Chapple, C., The origin and evolution of lignin biosynthesis. New Phytol., 2010, 187, 273–285.

  • Rogers, L. A. and Campbel, M. M., The genetic control of lignin deposition during plant growth and development. New Phytol., 2004, 164, 17–30.

  • Olenichenko, N. and Zagoskina, N., Response of winter wheat to cold: production of phenolic compounds and l-phenylalanine ammo-nia lyase activity. Appl. Biochem. Microbiol., 2005, 41, 600–603.

  • Janas, K. M., Cvikrova, M., Palagiewicz, A. and Eder, J., Alterations in phenylpropanoid content in soybean ischolar_mains during low tempera-ture acclimation. Plant Physiol. Biochem., 2000, 38, 587–593.

  • EI Kayal, W., Keller, G., Debayles, C., Kumar, R., Weier, D., Teulieres, C. and Marque, C., Regulation of tocopherol biosynthe-sis through transcriptional control of tocopherol cyclase during cold hardening in Eucalyptus gunnii. Physiol. Plantarum., 2006, 126, 212–223.

  • MacDonald, M. J. and D’Cunha, G. B., A modern view of phenyl-alanine ammonia lyase. Biochem. Cell Biol., 2007, 85, 273–282.

  • Valiñas, M. A., Lanteri, M. L., ten Have, A. and Balbina Andreu, A., Chlorogenic acid biosynthesis appears linked with suberin production in potato tuber (Solanum tuberosum). J. Agric. Food Chem., 2015, 63(19), 4902–4913.

  • Hisano, H., Nandakumar, R. and Wang, Z. Y., Genetic modifica-tion of lignin biosynthesis for improved biofuel production. In vitro Cell. Dev. Biol. (Plant), 2009, 45, 306–313.

  • Hu, R. et al., Comparative transcriptome analysis revealed the genotype specific cold response mechanism in tobacco. Biochem. Biophys. Res. Commun., 2016, 469(3), 535–541.

  • Wagner, A. et al., CCoAOMT suppression modifies lignin compo-sition in Pinus radiata. Plant J., 2011, 67, 119–129.

  • Guo, D., Chen, F., Inoue, K., Blount, J. W. and Dixon, R. A., Downregulation of caffeic acid 3-O-methyltransferase and caffeoyl CoA 3-O-methyltransferase in transgenic alfalfa: impacts on lignin structure and implications for the biosynthesis of G and S lignin. Plant Cell Online, 2001, 13, 73–88.

  • Guo, D., Chen, F., Wheeler, J., Winder, J., Selman, S., Peterson, M. and Dixon, R. A., Improvement of in-rumen digestibility of alfalfa forage by genetic manipulation of lignin O-methyl-transferases. Transgenic Res., 2001, 10, 457–464.

  • Zhong, R., Morrison, W. H., Himmelsbach, D. S., Poole, F. L. and Ye, Z. H., Essential role of caffeoyl coenzyme A O-methyltransferase in lignin biosynthesis in woody poplar plants. Plant Physiol., 2000, 124, 563–578.

  • Bonawitz, N. D. and Chapple, C., The genetics of lignin biosyn-thesis: connecting genotype to phenotype. Ann. Rev. Genet., 2010, 44(1), 337–363.

  • dos Santos, A. B. et al., Lignin biosynthesis in sugarcane is affected by low temperature. Environ. Exp. Bot., 2015, 120, 31–42.


Abstract Views: 223

PDF Views: 77




  • Transcriptomic Analysis of Chilling-treated Tobacco (Nicotiana tabacum) Leaves Reveals Chilling-Induced Lignin Biosynthetic Pathways

Abstract Views: 223  |  PDF Views: 77

Authors

PeiLu Zhou
Key Laboratory of Tobacco Biology and Processing, Ministry of Agriculture, Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao 266101, China
QiYao Li
Key Laboratory of Tobacco Biology and Processing, Ministry of Agriculture, Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao 266101, China
Guang Liang Liu
Key Laboratory of Tobacco Biology and Processing, Ministry of Agriculture, Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao 266101, China
Na Xu
Key Laboratory of Tobacco Biology and Processing, Ministry of Agriculture, Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao 266101, China
Yin Ju Yang
Key Laboratory of Tobacco Biology and Processing, Ministry of Agriculture, Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao 266101, China
Yi Wang
Key Laboratory of Tobacco Biology and Processing, Ministry of Agriculture, Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao 266101, China
Wen Long Zeng
Longyan Tobacco Agricultural Science Institute, Longyan, Fujian 364000,, China
Shu Sheng Wang
Key Laboratory of Tobacco Biology and Processing, Ministry of Agriculture, Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao 266101, China
Ai Guo Chen
Key Laboratory of Tobacco Biology and Processing, Ministry of Agriculture, Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao 266101, China

Abstract


Chilling stress is one of the most important environ-mental stresses for chilling-sensitive species. The pre-sent study conducted RNA-Seq and WGCNA analysis to clarify the correlation patterns among genes of dif-ferent treatments in tobacco (Nicotiana tabacum). A total of 10,355 DEGs were found in chilling treatment relative to control treatment. Additionally, functional annotations revealed that 48 genes were found to be specifically expressed in lignin biosynthesis pathway in tobacco seedlings under chilling stress. Our results revealed that the biosynthesis of caffeoyl-CoA was regulated by HCT and C3H. Furthermore, the G-type lignin biosynthesis branch was enhanced under low temperature, which contributed to an increase in lig-nin content and changes in lignin composition, indi-cating that G-type lignin may play an important role in tobacco’s resistance to chilling stress.

Keywords


Chilling Stress, Lignin Biosynthesis, Nicotiana Tabacum, Transcriptomic, WGCNA.

References





DOI: https://doi.org/10.18520/cs%2Fv117%2Fi11%2F1885-1892