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Biological Activity of Essential Oils of Mentha pulegium from Field-Grown and Acclimated in vitro Plants


Affiliations
1 Biotechnology Research Center (C.R.Bt), Nouvelle Ville – Ali Mendjeli 25000, Constantine, Algeria
2 Laboratory of Pharmacognosy, Faculty of Medicine, University Constantine 3, Nouvelle Ville – Ali Mendjeli 25000, Constantine, Algeria
 

In this study we discuss Mentha pulegium shoot multiplication and the capacity of in vitro plants to produce essential oils (EOs). EO was obtained by hydrodistillation from dried leaves and flowering stems of in vitro and field-grown plants, and their chemical composition was examined by GC-MS. The differences in yield as well as quantitative and qualitative composition between the oils extracted from in vitro and field-grown plants were observed. M. pulegium EOs were evaluated for their antioxidant and antimicrobial activities. It was found that antimicrobial activity of EOs extracted from acclimated in vitro plants was higher than those from field-grown M. pulegium plants. Our aim was to perform phytochemical screening of leaves from a wild-growing plant and compare the results with material produced using biotechnological methods based on tissue culture.

Keywords

Acclimatization, Biological Activities, Essential Oils, In vitro Plants, Mentha pulegium.
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  • Biological Activity of Essential Oils of Mentha pulegium from Field-Grown and Acclimated in vitro Plants

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Authors

Amira Benahmed
Biotechnology Research Center (C.R.Bt), Nouvelle Ville – Ali Mendjeli 25000, Constantine, Algeria
Boualem Harfi
Biotechnology Research Center (C.R.Bt), Nouvelle Ville – Ali Mendjeli 25000, Constantine, Algeria
Abdelmalik Belkhiri
Laboratory of Pharmacognosy, Faculty of Medicine, University Constantine 3, Nouvelle Ville – Ali Mendjeli 25000, Constantine, Algeria

Abstract


In this study we discuss Mentha pulegium shoot multiplication and the capacity of in vitro plants to produce essential oils (EOs). EO was obtained by hydrodistillation from dried leaves and flowering stems of in vitro and field-grown plants, and their chemical composition was examined by GC-MS. The differences in yield as well as quantitative and qualitative composition between the oils extracted from in vitro and field-grown plants were observed. M. pulegium EOs were evaluated for their antioxidant and antimicrobial activities. It was found that antimicrobial activity of EOs extracted from acclimated in vitro plants was higher than those from field-grown M. pulegium plants. Our aim was to perform phytochemical screening of leaves from a wild-growing plant and compare the results with material produced using biotechnological methods based on tissue culture.

Keywords


Acclimatization, Biological Activities, Essential Oils, In vitro Plants, Mentha pulegium.

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DOI: https://doi.org/10.18520/cs%2Fv116%2Fi11%2F1897-1904