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Apoptosis Time Window Induced by Cold Atmospheric Plasma:Comparison with Ionizing Radiation


Affiliations
1 Department of Physical Chemistry, Vinca Institute of Nuclear Sciences, University of Belgrade, Mike Petrovica Alasa 12-14, 11001 Belgrade, Serbia
2 Institute of Physics, University of Belgrade, Pregrevica 118, 11080 Belgrade, Serbia
 

In this study we evaluate apoptosis time window of primary fibroblasts treated with cold atmospheric plasma (CAP), power range 0.4–1.4 W, for 30 sec, using γ-H2AX phosphorylation assay and flow cytometry. In contrast to irradiation where maximum of γ-H2AX foci appeared 30 min after irradiation and apoptosis 24 h later irrespective of radiation dose, treatment with CAP (power of 0.4 and 0.6) induces maximum of γ-H2AX foci 2 h after treatment. Apoptosis occurred in a power-dependent manner, with time shift of 2–3 h. Besides power-dependent time shift in apoptosis induction, apoptosis time window is the same and lasts for 2 h.

Keywords

Apoptosis, Cold Atmospheric Plasma, Ionizing Radiation, Primary Human Fibroblasts, Repair Kinetics.
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  • Apoptosis Time Window Induced by Cold Atmospheric Plasma:Comparison with Ionizing Radiation

Abstract Views: 158  |  PDF Views: 59

Authors

Gordana Joksic
Department of Physical Chemistry, Vinca Institute of Nuclear Sciences, University of Belgrade, Mike Petrovica Alasa 12-14, 11001 Belgrade, Serbia
Ana Valenta Šobot
Department of Physical Chemistry, Vinca Institute of Nuclear Sciences, University of Belgrade, Mike Petrovica Alasa 12-14, 11001 Belgrade, Serbia
Jelena Filipovic Trickovic
Department of Physical Chemistry, Vinca Institute of Nuclear Sciences, University of Belgrade, Mike Petrovica Alasa 12-14, 11001 Belgrade, Serbia
Dejan Maletic
Institute of Physics, University of Belgrade, Pregrevica 118, 11080 Belgrade, Serbia
Nevena Puac
Institute of Physics, University of Belgrade, Pregrevica 118, 11080 Belgrade, Serbia
Gordana Malovic
Institute of Physics, University of Belgrade, Pregrevica 118, 11080 Belgrade, Serbia
Zoran Lj. Petrovic
Institute of Physics, University of Belgrade, Pregrevica 118, 11080 Belgrade, Serbia
Sasa Lazovic
Institute of Physics, University of Belgrade, Pregrevica 118, 11080 Belgrade, Serbia

Abstract


In this study we evaluate apoptosis time window of primary fibroblasts treated with cold atmospheric plasma (CAP), power range 0.4–1.4 W, for 30 sec, using γ-H2AX phosphorylation assay and flow cytometry. In contrast to irradiation where maximum of γ-H2AX foci appeared 30 min after irradiation and apoptosis 24 h later irrespective of radiation dose, treatment with CAP (power of 0.4 and 0.6) induces maximum of γ-H2AX foci 2 h after treatment. Apoptosis occurred in a power-dependent manner, with time shift of 2–3 h. Besides power-dependent time shift in apoptosis induction, apoptosis time window is the same and lasts for 2 h.

Keywords


Apoptosis, Cold Atmospheric Plasma, Ionizing Radiation, Primary Human Fibroblasts, Repair Kinetics.

References





DOI: https://doi.org/10.18520/cs%2Fv116%2Fi7%2F1229-1233