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An Efficient Method for Acclimatization:in vitro Hardening of Tissue Culture-Raised Tea Plants (Camellia sinensis (l.) O. Kuntze)


Affiliations
1 G.B. Pant National Institute of Himalayan Environment and Sustainable Development, Kosi-Katarmal, Almora 263 643, India
2 Present address: Graphic Era Deemed to be University, Clement Town, Dehradun 248 002, India
 

In the 21st century while there is all-round development in every sphere of science and technology, a major limitation in micropropagation technology still remains; a large number of plantlets of several species die during hardening and ex vitro transfer. In the present study, introducing an in vitro incubation period in plant growth regulator (PGR)-free medium followed by ischolar_main induction of micropropagated tea shoots (Camellia sinensis (L.) O. Kuntze) and increasing it to 12 weeks instead of the usual 4 weeks gave excellent results. Secondly, cultures were grown in larger vessels, i.e. in 500 ml conical flasks in place of normal 250 ml conical flasks. Interestingly, prolonged incubation (12 weeks) significantly enhanced overall growth, i.e. length, stem girth, leaf number and leaf area of plantlets. The use of larger-sized culture flask also positively influenced growth. The abovementioned two simple modifications in culture conditions improved overall growth of plantlets. Also, plantlets raised through prolonged culture were acclimatized with 94% survival efficiency in comparison to 46% with normal culture period, and showed improved growth performance on transfer to soil in the nursery, irrespective of the culture vessel size.

Keywords

Acclimatization, in vitro Hardening, Lab-to-Land Transfer, Prolonged Culture, Tea Micropropagation.
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  • An Efficient Method for Acclimatization:in vitro Hardening of Tissue Culture-Raised Tea Plants (Camellia sinensis (l.) O. Kuntze)

Abstract Views: 243  |  PDF Views: 72

Authors

Niladri Bag
G.B. Pant National Institute of Himalayan Environment and Sustainable Development, Kosi-Katarmal, Almora 263 643, India
Lok Man S. Palni
Present address: Graphic Era Deemed to be University, Clement Town, Dehradun 248 002, India
Shyamal Kumar Nandi
G.B. Pant National Institute of Himalayan Environment and Sustainable Development, Kosi-Katarmal, Almora 263 643, India

Abstract


In the 21st century while there is all-round development in every sphere of science and technology, a major limitation in micropropagation technology still remains; a large number of plantlets of several species die during hardening and ex vitro transfer. In the present study, introducing an in vitro incubation period in plant growth regulator (PGR)-free medium followed by ischolar_main induction of micropropagated tea shoots (Camellia sinensis (L.) O. Kuntze) and increasing it to 12 weeks instead of the usual 4 weeks gave excellent results. Secondly, cultures were grown in larger vessels, i.e. in 500 ml conical flasks in place of normal 250 ml conical flasks. Interestingly, prolonged incubation (12 weeks) significantly enhanced overall growth, i.e. length, stem girth, leaf number and leaf area of plantlets. The use of larger-sized culture flask also positively influenced growth. The abovementioned two simple modifications in culture conditions improved overall growth of plantlets. Also, plantlets raised through prolonged culture were acclimatized with 94% survival efficiency in comparison to 46% with normal culture period, and showed improved growth performance on transfer to soil in the nursery, irrespective of the culture vessel size.

Keywords


Acclimatization, in vitro Hardening, Lab-to-Land Transfer, Prolonged Culture, Tea Micropropagation.

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DOI: https://doi.org/10.18520/cs%2Fv117%2Fi2%2F288-293