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Microbial Eukaryotic Diversity with Emphasis on Picoprasinophytes under the Sea Ice of the Central Arctic Ocean in Summer


Affiliations
1 Polar Research Institute of China, Ministry of Natural Resources, Shanghai 200136, China
 

The central Arctic Ocean and its microbial ecosystem are shifting towards variable states due to climate change. In this study, diverse microbial eukaryotes belonging to Alveolata, Chlorophyta, Stramenopile, Telonemia, Picobiliphyta, Cercozoa, Choanoflagellida, Fungi and Haptophyta have been identified by pyrosequencing. Canonical correspondence analysis suggested that the microbial eukaryote communities sampled from lower latitudes were significantly correlated with temperature and nutrients in sea water, whereas those from higher latitudes were correlated with conditions of ice cover, latitude of sample site and chlorophyll concentration. Picoplankton Micromonas, with a total occurrence of 17% of all reads, was the most abundant taxon. Quantification of picoprasinophytes by FISH proved their absolute predominance in the central Arctic Ocean under heavy sea ice.

Keywords

Microbial Eukaryotes, Molecular Probe, Picoprasinophyte, Sea Ice
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  • Not, F. et al., Picobiliphytes: a marine picoplanktonic algal group with unknown affinities to other eukaryotes. Science, 2007, 315, 253–255.

  • Luo, W., Li, H. R., Cai, M. H. and He, J. F., Diversity of microbial eukaryotes in Kongsfjorden, Svalbard. Hydrobiologia, 2009, 636, 233–248.

  • Piquet, A. M. T., Scheepens, J. F., Bolhuis, H., Wiencke, C. and Buma, A. G. J., Variability of protistan and bacterial communities in two Arctic fjords (Spitsbergen). Polar Biol., 2010, 33, 1521– 1536.

  • Bachy, C., López-García, P., Vereshchaka, A. and Moreira, D., Diversity and vertical distribution of microbial eukaryotes in the snow, sea ice and seawater near the North Pole at the end of the polar night. Front. Microbiol., 2011, 2, 1–12.

  • Comeau, A. M., Li, W. K. W., Tremblay, J.-É., Carmack, E. C. and Lovejoy, C., Arctic Ocean microbial community structure before and after the 2007 record sea ice minimum. PLoS ONE, 2011, 6(11), 1–12.

  • Kilias, E. S., Peeken, I. and Metfies, K., Insight into protist diversity in Arctic sea ice and melt-pond aggregate obtained by pyrosequencing. Polar Res., 2014, 33, 1–10.

  • Féral, J. P., How useful are the genetic markers in attempts to understand and manage marine biodiversity? J. Exp. Mar. Biol. Ecol., 2002, 268, 121–145.

  • Lovejoy, C., Vincent, W. F., Bonilla, S. and Roy, S., Distribution, phylogeny, and growth of cold-adapted picoprasinophytes in Arctic seas. J. Phycol., 2007, 43, 78–89.

  • Lim, E. L., Amaral, L. A., Caron, D. A. and DeLong, E. F., Application of rRNA-based probes for observing marine nanoplanktonicprotists. Appl. Environ. Microbiol., 1993, 59, 1647–1655.

  • Biegala, I. C., Not, F., Vaulot, D. and Simon, N., Quantitative assessment of picoeukaryotes in the natural environment by using taxon-specific oligonucleotide probes in association with tyramide signal amplification-fluorescent in situ hybridization and flow cytometry. Appl. Environ. Microbiol., 2003, 69, 5519–5529.

  • Worden, A. Z., Picoeukaryote diversity in coastal waters of the Pacific Ocean. Aquat. Microb. Ecol., 2006, 43, 165–175.

  • Gao, X. Y., Li, Y. G., Li, H. R. and Luo, W., Optimization of fluorescence in situ hybridization (FISH) for the identification of two polar coccoid green algae species. Chin. J. Polar Sci., 2010, 21(2), 167–179.

  • Foulon, E., Not, F., Jalabert, F., Cariou, T., Massana, R. and Simon, N., Ecological niche partitioning in the picoplanktonic green alga Micromonas pusilla: evidence from environmental surveys using phylogenetic probes. Environ. Microbiol., 2008, 10(9), 2433–2443.

  • Vincent, W. F., Microbial ecosystem responses to rapid climate change in the Arctic. ISME J., 2010, 4, 1089–1091.


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  • Microbial Eukaryotic Diversity with Emphasis on Picoprasinophytes under the Sea Ice of the Central Arctic Ocean in Summer

Abstract Views: 217  |  PDF Views: 68

Authors

Wei Luo
Polar Research Institute of China, Ministry of Natural Resources, Shanghai 200136, China
Huirong Li
Polar Research Institute of China, Ministry of Natural Resources, Shanghai 200136, China
Xiaoyan Gao
Polar Research Institute of China, Ministry of Natural Resources, Shanghai 200136, China
Ling Lin
Polar Research Institute of China, Ministry of Natural Resources, Shanghai 200136, China
Yong Yu
Polar Research Institute of China, Ministry of Natural Resources, Shanghai 200136, China
Yinxin Zeng
Polar Research Institute of China, Ministry of Natural Resources, Shanghai 200136, China

Abstract


The central Arctic Ocean and its microbial ecosystem are shifting towards variable states due to climate change. In this study, diverse microbial eukaryotes belonging to Alveolata, Chlorophyta, Stramenopile, Telonemia, Picobiliphyta, Cercozoa, Choanoflagellida, Fungi and Haptophyta have been identified by pyrosequencing. Canonical correspondence analysis suggested that the microbial eukaryote communities sampled from lower latitudes were significantly correlated with temperature and nutrients in sea water, whereas those from higher latitudes were correlated with conditions of ice cover, latitude of sample site and chlorophyll concentration. Picoplankton Micromonas, with a total occurrence of 17% of all reads, was the most abundant taxon. Quantification of picoprasinophytes by FISH proved their absolute predominance in the central Arctic Ocean under heavy sea ice.

Keywords


Microbial Eukaryotes, Molecular Probe, Picoprasinophyte, Sea Ice

References





DOI: https://doi.org/10.18520/cs%2Fv115%2Fi9%2F1709-1713