Current Science

Open Access Open Access  Restricted Access Subscription Access

Molecular Characterization of Potassium Solubilizing Bacteria from Crop Rhizosphere of the North Eastern Region of India


Affiliations
1 Department of Soil Science, Assam Agricultural University, Jorhat 785 013, India
2 Department of Entomology, Assam Agricultural University, Jorhat 785 013, India
 

In this study, isolation and characterization of potassium solubilizing bacteria (KSB) have been carried out which could solubilize a substantial amount of potassium (K) following incubation with mica. Five KSB, isolated from banana and chilli rhizosphere were identified by 16S rDNA sequence analysis. The identified bacteria were Klebsiella sp. and Bacillus cereus. The isolates had K solubilization potential within the range of 76.3–78.42% compared to control along with significant drop in pH (4.62–4.86). The study reveals the exploitation of Klebsiella sp. and B. cereus as potential K solubilizing biofertilizers.

Keywords

Crop Rhizosphere, Molecular Characterization, Mica, Potassium Solubilizing Bacteria.
User
Notifications
Font Size

  • Sparks, D. L., Potassium dynamics in soils. Adv. Soil Sci., 1987, 6, 1–63.

  • Pettigrew, W. T., Potassium influences on yield and quality production for maize, wheat, soybean and cotton. Physiol. Plant, 2008, 133, 670–681.

  • Naidu, L. G. K., Ramamurthy, V., Sindhu, G. S. and Sarkar, D., Emerging deficiency of potassium in soils and crops of India, Karnataka. J. Agric. Sci., 2011, 24(1), 12–19.

  • Zhang, F., Wang, J., Zhang, W., Cui, Z., Ma, W., Chen, X. and Jiang, R., Nutrient use efficiency of major cereal crops in China. Acta Pedol. Sin., 2008, 45, 915–922.

  • Malinovskaya, I. M., Kosenko, L. V., Votselko, S. K. and Podgorskii, V. S., Role of Bacillus mucilaginosus polysaccharide in degradation of silicate minerals. Microbiology, 1990, 59, 49–55.

  • Friedrich, S., Platonova, N. P., Karavaiko, G. I., Ztichel, E. and Glombitza, F., Chemical and microbiological solubilisation of silicates. Acta Biotechnol., 2004, 11, 187–196.

  • Liu, D., Lian, B. and Dong, H., Isolation of Paenibacillus sp. and assessment of its potential for enhancing mineral weathering. Geomicrobiology, 2012, 29, 413–421.

  • Richards, J. E. and Bates, T. E., Studies on the potassiumsupplying capacities of southern Ontario soils. III. Measurement of available K. Can. J. Soil Sci., 1989, 69(3), 597–610.

  • Barker, W. W., Welch, S. A., Chu, S. and Banfield, J. F., Experimental observations of the effects of bacteria on alluminosilicate weathering. Am. Mineral., 1998, 83, 1551–1563.

  • Rajaram, K. and Suri, R. K., In Science and Technology in India, Spectrum Publication, 2000, pp. 68–69.

  • Sugumaran, P. and Janarthanam, B., Solubilization of potassium containing minerals by bacteria and their effect on plant growth. World J. Agric. Res., 2007, 3, 350–355.

  • Archana, D. S., Nandish, M. S., Savalagi, V. P. and Alagawadi, A. R., Characterization of potassium solubilizing bacteria (KSB) from rhizosphere soil. BIOINFOLET, 2013, 10, 248–257.

  • Prajapati, K. B. and Modi, H. A., Isolation and characterization of potassium solubilising bacteria from ceramic industry soil. CIBTech. J. Microbiol., 2012, 1(2–3), 8–14.

  • Sangeeth, K. P., Suseela Bhai, R. and Srinivasan, V., Paenibacillus glucanolyticus, a promising potassium solubilizing bacterium isolated from black pepper (Piper nigrum L.) rhizosphere. J. Spice Aromat. Crops, 2012, 21(2), 118–124.

  • Dutta, S. and Shanwal, A. V., Potassium bearing minerals in some soils of semi-arid (Haryana) and humid (Assam) regions of India. Agropedology, 2006, 16, 86–91.

  • Hu, X. F., Chen, J. and Guo, J. F., Two phosphate- and potassium-solubilizing bacterial isolated from Tiannu Mountain, Zhijiang, China. World J. Microbiol. Biotechnol., 2006, 22, 983–990.

  • Basak, B. B. and Biswas, D. R., Influence of potassium solubilizing microorganism (Bacillus mucilaginosus) and waste mica on potassium uptake dynamics by Sudan grass (Sorghum vulgare Pers.) grown under two Alfisols. Plant Soil, 2009, 317, 235–255.

  • Sheng, X. F., Zhao, F., He, H., Qiu, G. and Chen, L., Isolation, characterization of silicate mineral solubilizing Bacillus globisporus Q12 from the surface of weathered feldspar. Can. J. Microbiol., 2008, 54, 1064–1068.

  • Zhang, C. and Kong, F., Isolation and identification of potassiumsolubilizing bacteria from tobacco rhizospheric soil and their effect on tobacco plants. Appl. Soil Ecol., 2014, 82, 18–25.

  • Maurya, B. R., Meena, V. S. and Meena, O. P., Influence of Inceptisol and Alfisol’s potassium solubilizing bacteria (KSB) isolates on release of K from waste mica. Vegetos, 2014, 27(1), 181–187.

  • Meena, V. S., Maurya, B. R., Verma, J. P., Aeron, A., Kumar, A., Kangmin, K. K. and Bajpai, V. K., Potassium solubilizing rhizobacteria (KSR): isolation, identification, and K-release dynamics from waste mica. Ecol. Eng., 2015, 81, 340–347.

  • Zhao, F., Sheng, X., Huang, Z. and He, L., Isolation of mineral potassium solubilizing bacterial strains from agricultural soils in Shandong Province. Biodivers. Sci., 2008, 16(6), 593–600.

  • Binbin, M. O. and Bin, L., Interaction between Bacillus mucilaginous and silicate minerals (weathered adamellite and feldspar): weathering rate, products, and reaction mechanism. Chin. J. Geochem., 2011, 30, 187–192.

  • Zarjanji, J. K., Aliasgharzad, N., Oustan. S., Emadi, M. and Ahmadi, A., Isolation and characterization of potassium solubilizing bacteria in some Iranian soils. Arch. Agron. Soil Sci., 2013, 59(12), 1–11.

  • Leyval, C. and Berthelin, J., Interactions between Laccaria laccata, Agrobacterium radiobacter and beech ischolar_mains: influence on P, K, Mg and Fe mobilization from minerals and plant growth. Plant Soil, 1989, 117, 103–110.

  • Biswas, D. R. and Basak, B. B., Mobilization of potassium from waste mica by potassium-solubilizing bacteria (Bacillus mucilaginosus) as influenced by temperature and incubation period under in vitro laboratory conditions. Agrochimica LVIII (4), 2014, 309–320.


Abstract Views: 194

PDF Views: 84




  • Molecular Characterization of Potassium Solubilizing Bacteria from Crop Rhizosphere of the North Eastern Region of India

Abstract Views: 194  |  PDF Views: 84

Authors

Smrita Buragohain
Department of Soil Science, Assam Agricultural University, Jorhat 785 013, India
Dhruba Jyoti Nath
Department of Soil Science, Assam Agricultural University, Jorhat 785 013, India
Yumnam Bijilaxmi Devi
Department of Soil Science, Assam Agricultural University, Jorhat 785 013, India
Badal Bhattacharyya
Department of Entomology, Assam Agricultural University, Jorhat 785 013, India
Samiron Dutta
Department of Soil Science, Assam Agricultural University, Jorhat 785 013, India

Abstract


In this study, isolation and characterization of potassium solubilizing bacteria (KSB) have been carried out which could solubilize a substantial amount of potassium (K) following incubation with mica. Five KSB, isolated from banana and chilli rhizosphere were identified by 16S rDNA sequence analysis. The identified bacteria were Klebsiella sp. and Bacillus cereus. The isolates had K solubilization potential within the range of 76.3–78.42% compared to control along with significant drop in pH (4.62–4.86). The study reveals the exploitation of Klebsiella sp. and B. cereus as potential K solubilizing biofertilizers.

Keywords


Crop Rhizosphere, Molecular Characterization, Mica, Potassium Solubilizing Bacteria.

References





DOI: https://doi.org/10.18520/cs%2Fv114%2Fi12%2F2543-2548