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In vitro and In silico Validation of Anti-Cobra Venom Activity and Identification of Lead Molecules in Aegle marmelos (L.) Correa


Affiliations
1 Saraswathy Thangavelu Centre, Biotechnology and Bioinformatics Division, Jawaharlal Nehru Tropical Botanic Garden and Research Institute, Puthenthope, Thiruvananthapuram 695 586, India
2 Department of Zoology, University College, Thiruvananthapuram 695 034, India
 

Venomous snakebite is a global serious health issue and in India high rate of mortality is caused by Naja naja (Indian cobra). To evaluate anti-cobra venom activity and identify lead molecules in Aegle marmelos, in vitro and in silico screening was carried out. Leaves, stem and ischolar_main bark of A. marmelos were extracted in ethanol, methanol and hexane and maximum yield was obtained in methanol. All extracts were used for testing in vitro anti-haemolytic, inhibition of anti-acetylcholinesterase and anti-proteolytic activities. The results revealed that ethanol extract of ischolar_main bark has high anti-haemolytic activity, methanol extracts of leaves have the highest inhibitory effect on venom induced anti-acetylcholinesterase activity and ethanol extracts of leaves have maximum anti-proteolytic activity. Docking between 81 phytochemicals from A. marmelos and each of the 14 cobra venom toxic proteins revealed that the plant contains potential molecules for detoxification of all the cobra venom proteins.

Keywords

Aegle marmelos, Cobra Venom, Docking, Phytochemicals, Snakebite.
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  • Mohapatra, B., Warrell, D. A., Suraweera, W., Bhatia, P. and Dhingra, N., Snakebite mortality in India: A nationally representative mortality survey. PLoS. Negl. Trop. Dis., 2011, 5, e1018.

  • Kasturiratne, A. et al., The global burden of snakebite: A literature analysis and modelling based on regional estimates of envenoming and deaths. PLoS Med., 2008, 5, e218.

  • Lalla, J. K., Sunita, O., Priyanka, G., Zaid, T. and Geeta T., Snakebite problem in India: An overview. Sch. Acad. J. Pharm., 2013, 2, 252–259.

  • Nisha, N. C., Sreekumar, S., Biju, C. K. and Krishnan, P. N., Snake antivenom: Virtual screening of plant derived molecules. Biobytes, 2010, 6, 14–22.

  • Vyas, V. K., Brahmbhatt, K., Bhatt, H. and Parmar, U., Therapeutic potential of snake venom in cancer therapy: current perspectives. Asian Pac. J. Trop. Biomed., 2013, 3, 156–162.

  • Binh, D. V., Thanh, T. T. and Chi, P. V., Proteomic characterization of the thermostable toxins from Naja naja venom. J. Venom. Anim. Toxins Incl. Trop. Dis., 2010, 16, 631–638.

  • Singh, K. K., Kalakoti, B. S. and Prakash A., Traditional phytotherapy in the healthcare of Gond tribals of Sonbhadra district, UttarPradesh, India. J. Bombay Nat. Hist. Soc., 1994, 91, 385–390.

  • Raju, M. S., Native plants used in snakebite and other poisonous animals among the tribals of East Godavari district, Andhra Pradesh. Aryavaidyan, 1996, 9, 251–255.

  • Kirtikar, K. R. and Basu, B. D., Indian medicinal plants. In second edition (eds Balatter, E., Caius, J. F. and Mhaskar, K. S), Bishen Singh Mahendra Pal Singh, Dehradun, India, 2004, pp. 499–502.

  • Vijayabharathi, R. A., Kumar, S. and Kumar, K. S., In vitro and in vivo anti-snake venom activity of Coccinia indica L. leaf. Hamdard Med., 2005, 48, 132–135.

  • Alsever, J. B. and Ainslie, R. B., A new method for the preparation of dilute blood plasma and the operation of a complete transfusion service. N. Y. State J. Med., 1941, 41, 126–131.

  • Augustinson, Assay methods for cholinesterase. In Methods of Biochemical analysis (ed. Glick, D.), Wiley, New York, 1957, p. 43.

  • Mycek, M. J., Cathepsin methods. Enzymology, 1970, 9, 285–315.

  • Nisha, N. C., Sreekumar, S., Biju, C. K. and Krishnan, P. N., Identification of lead compounds with cobra venom neutralizing activity in three Indian medicinal plants. Int. J. Pharm. Pharm. Sci., 2014, 6, 536–541.

  • Peitsch, M. C., Protein modeling by e-mail Bio/Technology. Nat. Biotechnol., 1995, 13, 658–660.

  • Morris, G. M., Huey, R., Lindstrom, W., Sanner, M. F., Belew, R. K., Goodsell, D. S. and Olson, A. J., AutoDock4 and AutoDock-Tools4: Automated docking with selective receptor flexibility. J. Comput. Chem., 2009, 30, 2785–2791.

  • Nisha, N. C., Sreekumar, S. and Biju, C. K., Identification of lead compounds with cobra venom detoxification activity in Andrographis paniculata (Burm. F.) Nees through in silico method. Int. J. Pharm. Pharm. Sci., 2016, 8, 212–217.

  • http://www.molinspiration.com

  • Mitul, P., Handral, M. and Swaroop, T. V. S. S., Anti-venom activity of Cinnamomum zeylanicum extracts against Naja kauthia snake venom. Int. J. Pharm. Chem. Biol. Sci., 2013, 2, 1302–1310.

  • Maiorano, V. A. et al., Antiophidian properties of the aqueous extract of Mikania glomerata. J. Ethnopharmacol., 2005, 102, 364–370.

  • Alam, M. I. and Gomes, A., Snake venom neutralization by Indian medicinal plants (Vitex negundo and Emblica officinalis) ischolar_main extracts. J. Ethnopharmacol., 2003, 86, 75–80.

  • Monimala, M. and Mukherjee, A. K., Neutralisation of lethality, myotoxicity and toxic enzymes of Naja kaouthia venom by Mimosa pudica ischolar_main extracts. J. Ethnopharmacol., 2001, 75, 55–60.

  • Soto, J. G., Perez, J. C. and Minton, S. A., Proteolytic, hemorrhagic and hemolytic activities of snake venoms. Toxicon, 1988, 26, 875–882.

  • Janardhan, B., Shrikanth, V. M., Mirajkar, K. K. and More, S. S., In vitro screening and evaluation of antivenom phytochemicals from Azima tetracantha Lam. leaves against Bungarus caeruleus and Vipera russelli. J. Venom. Anim. Toxins Incl. Trop. Dis., 2014, 1, 1.


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  • In vitro and In silico Validation of Anti-Cobra Venom Activity and Identification of Lead Molecules in Aegle marmelos (L.) Correa

Abstract Views: 322  |  PDF Views: 82

Authors

N. C. Nisha
Saraswathy Thangavelu Centre, Biotechnology and Bioinformatics Division, Jawaharlal Nehru Tropical Botanic Garden and Research Institute, Puthenthope, Thiruvananthapuram 695 586, India
S. Sreekumar
Saraswathy Thangavelu Centre, Biotechnology and Bioinformatics Division, Jawaharlal Nehru Tropical Botanic Garden and Research Institute, Puthenthope, Thiruvananthapuram 695 586, India
D. A. Evans
Department of Zoology, University College, Thiruvananthapuram 695 034, India
C. K. Biju
Saraswathy Thangavelu Centre, Biotechnology and Bioinformatics Division, Jawaharlal Nehru Tropical Botanic Garden and Research Institute, Puthenthope, Thiruvananthapuram 695 586, India

Abstract


Venomous snakebite is a global serious health issue and in India high rate of mortality is caused by Naja naja (Indian cobra). To evaluate anti-cobra venom activity and identify lead molecules in Aegle marmelos, in vitro and in silico screening was carried out. Leaves, stem and ischolar_main bark of A. marmelos were extracted in ethanol, methanol and hexane and maximum yield was obtained in methanol. All extracts were used for testing in vitro anti-haemolytic, inhibition of anti-acetylcholinesterase and anti-proteolytic activities. The results revealed that ethanol extract of ischolar_main bark has high anti-haemolytic activity, methanol extracts of leaves have the highest inhibitory effect on venom induced anti-acetylcholinesterase activity and ethanol extracts of leaves have maximum anti-proteolytic activity. Docking between 81 phytochemicals from A. marmelos and each of the 14 cobra venom toxic proteins revealed that the plant contains potential molecules for detoxification of all the cobra venom proteins.

Keywords


Aegle marmelos, Cobra Venom, Docking, Phytochemicals, Snakebite.

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DOI: https://doi.org/10.18520/cs%2Fv114%2Fi06%2F1214-1221