Current Science

Open Access Open Access  Restricted Access Subscription Access

Optimization of Key Factors for Enhanced Fermentative Biohydrogen Production from Water Hyacinth by RSM


Affiliations
1 School of Studies in Biotechnology, Pandit Ravishankar Shukla University, Raipur 492 010, India
 

This communication discusses the optimization of key factors for the enhanced bio-hydrogen production from water hyacinth. Three critical factors inoculums age (18-24 h), inoculums volume (20-80 ml/l) and concentration of sulphuric acid (0.5-2.0%) were optimized by response surface methodology (RSM) with central composite design (CCD) for better production. RSM analysis showed that all three factors significantly influenced hydrogen production. The optimum hydrogen production was 705 ml/l obtained with 21 h old bacterial culture, 50 ml/l inoculums with 1.25% sulphuric acid pre-treatment. The hydrogen concentration produced by Clostridium acetobutylicum NCIM 2877 was enhanced after using RSM. The results obtained indicate that RSM with CCD can be used as a technique to optimize culture conditions for enhancement of hydrogen production by pre-treatment of low-cost organic substrate; water hyacinth using dark fermentation methods may be one of the most promising approaches.

Keywords

Central Composite Design, Clostridium acetobutylicum NCIM 2877, Hydrogen Production, Response Surface Methodology, Water-Hyacinth.
User
Notifications
Font Size

  • Fang, H. H. P., Zhu, H. G. and Zhang, T., Phototrophic hydrogen production from glucose by pure and co-cultures of Clostridium butyricum and Rhodobacter sphaeroides. Int. J. Hydrogen Energ., 2006, 31(115), 2223–2230.

  • Guragain, Y., Coninck, J. D., Husson, F., Durand, A. and Rakshit, S. K., Comparison of some new pretreatment methods for second generation bioethanol production from wheat straw and water hyacinth. Bioresource Technol., 2011, 102, 4416–4424.

  • Lin, C. Y., Lay, C. H., Sen, B., Chu, C. Y., Kumar, G., Chen, C. C. and Chang, J. S., Fermentative hydrogen production from wastewaters: a review and prognosis. Int. J. Hydrogen Energ., 2012, 37(2), 15632–15642.

  • Cheng, J., Su, H., Zhou, J., Song, W. and Cen, K., Hydrogen production by mixed bacteria through dark and photo fermentation. Int. J. Hydrogen Energ., 2011, 36, 450–457.

  • Gunnarsson, C. C. and Petersen, C. M., Water hyacinths as a resource in agriculture and energy production: a literature review. Waste Manage., 2007, 27(1), 117–129.

  • Moretti, M. M. et al., Pretreatment of sugarcane bagasse with microwaves irradiation and its effects on the structure and on enzymatic hydrolysis. Appl. Energy, 2014, 122, 189–195.

  • Zhang, H. and Wu, S., Dilute ammonia pretreatment of sugarcane bagasse followed by enzymatic hydrolysis to sugars Cellulose, 2014, 21, 1341–1349.

  • Zhao, X., Xing, D., Fu, N., Liu, B. and Ren, N., Hydrogen production by the newly isolated Clostridium beijerinckii RZF-1108. Bioresour. Technol., 2011, 102(18), 8432–8436.

  • Zheng, Y., Zhao, J., Xu, F. and Li, Y., Pretreatment of lignocellulosic biomass for enhanced biogas production. Prog. Energy Combust. Sci., 2014, 42, 35–53.

  • Ma, F., Yang, N., Xu, C., Yu, H., Wu, J. and Zhang, X. Combination of biological pretreatment with mild acid pretreatment for enzymatic hydrolysis and ethanol production from water hyacinth. Bioresour. Technol., 2010, 101, 9600–9604.

  • Cheng, J., Su, H., Zhou, J., Song, W. and Cen, K., Hydrogen production by mixed bacteria through dark and photo fermentation. Int. J. Hydrogen Energ., 2011, 36, 450–457.

  • Zhang, J., Sun, H., Pan, C., Fan, Y. and Hou, H., Optimimization of process parameters for directly converting raw corn stalk to biohydrogen by Clostridium sp. FZ11 without substrate pretreatment. Energy Fuels, 2016, 30(1) 311–317.

  • Kotay, S. M. and Das, D., Microbial hydrogen production with Bacillus coagulans IIT-BT S1 isolated from anaerobic sewage sludge. Bioresour. Technol., 2007, 98, 1183–1190.

  • Jaapar, S. Z. S., Mohd. Kalil, S, Ali, E. and Nurina, A., Effects of age of inoculum and headspace on hydrogen production using Rhodobacter sphaeroides. Bacteriol. J., 2011, 1–8.

  • Prakashan, R. S., Sathish, T. and Brahmaiah, P., Biohydrogen production process optimization using anaerobic mixed consortia: a prelude study for use of agro-industrial material hydrolysate as substrate. Bioresour. Technol., 2010, 101, 5708–5711.

  • Ferchichi, M. E., Crabbe, W., Hintz, G. H., Gil and Almadidy, A., Influence of culture parameters on biological hydrogen production by Clostridium saccharoperbutylacetonicum ATCC 27021 World J. Microbiol. Biotechnol., 2005, 21, 855–862.

  • Mannikadan, T. R., Dhanasekar, R. and Thirumavalan, K., Microbial production of hydrogen from sugarcane Bagasse using Bacillus sp. Int. J. Chem. Technol. Res., 2009, 1(2), 344–348.

  • Cheng, J., Lin, R., Song, W., Xia, A., Zhou, J. and Cen, K., Enhancement of fermentative hydrogen production from hydrolysed water hyacinth with activated carbon detoxification and bacteria domestication. Int. J. Hydrogen Energ., 2015, 40(6), 2545–2551.

  • Pattra, S., Sangyoka, S., Boonmee, M. and Reungsang, A., Sugarcane bagasse (SCB) used in hydrogen production by Clostridium butyricum was hydrolyzed using H2SO4 at various concentration. Int. J. Hydrogen Energ., 2008, 33(19) 5256–5265.


Abstract Views: 205

PDF Views: 72




  • Optimization of Key Factors for Enhanced Fermentative Biohydrogen Production from Water Hyacinth by RSM

Abstract Views: 205  |  PDF Views: 72

Authors

Veena Thakur
School of Studies in Biotechnology, Pandit Ravishankar Shukla University, Raipur 492 010, India
Mona Tandon
School of Studies in Biotechnology, Pandit Ravishankar Shukla University, Raipur 492 010, India
S. K. Jadhav
School of Studies in Biotechnology, Pandit Ravishankar Shukla University, Raipur 492 010, India

Abstract


This communication discusses the optimization of key factors for the enhanced bio-hydrogen production from water hyacinth. Three critical factors inoculums age (18-24 h), inoculums volume (20-80 ml/l) and concentration of sulphuric acid (0.5-2.0%) were optimized by response surface methodology (RSM) with central composite design (CCD) for better production. RSM analysis showed that all three factors significantly influenced hydrogen production. The optimum hydrogen production was 705 ml/l obtained with 21 h old bacterial culture, 50 ml/l inoculums with 1.25% sulphuric acid pre-treatment. The hydrogen concentration produced by Clostridium acetobutylicum NCIM 2877 was enhanced after using RSM. The results obtained indicate that RSM with CCD can be used as a technique to optimize culture conditions for enhancement of hydrogen production by pre-treatment of low-cost organic substrate; water hyacinth using dark fermentation methods may be one of the most promising approaches.

Keywords


Central Composite Design, Clostridium acetobutylicum NCIM 2877, Hydrogen Production, Response Surface Methodology, Water-Hyacinth.

References





DOI: https://doi.org/10.18520/cs%2Fv113%2Fi04%2F790-795