Open Access Open Access  Restricted Access Subscription Access

Isolation and Characterization of Phosphorus Solubilizing Bacteria from Manganese Mining Area of Balaghat and Chhindwara


Affiliations
1 Department of Biological Sciences and Engineering, Maulana Azad National Institute of Technology, Bhopal 462 003, India
2 Department of Mechanical Engineering, National Institute of Technology Suratkal, Suratkal 575 025, India
 

Plants require optimum amount of available phosphorus to support their growth and development. Phosphorus is known to have significant role in ischolar_main subdivision, vitality and disease resistance of plants. Different types of bacteria involved in phosphorus solubilization can be used as biofertilizer in reclamation of mining area. The present study deals with isolation and identification of phosphorus solubilizing bacteria from the manganese mining area of Balaghat and Chhindwara districts of Madhya Pradesh, India. rDNA (16s) based molecular identification was performed assisted by MEGA phylogenetic analysis. Pseudomonas putida, Bacillus licheniformis, Pseudomonas taiwanensis and Pseudomonas aeruginosa were explored as potential phosphorus solubilizers from the selected sites.

Keywords

Mining Area, Phosphorus Solubilizing Bacteria, 16s rDNA.
User
Notifications
Font Size

  • Jungk, A., Seeling, B. and Gerke, J., Mobilization of different phosphate fractions in the rhizosphere. Plant Soil, 1993, 1, 91–94.
  • Raghothama, K. G., Phosphate acquisition. Annu. Rev. Plant Biol., 1999, 50, 665–693.
  • Yadav, H., Gothwal, R. K., Nigam, V. K., Sinha-Roy, S. and Ghosh, P., Optimization of culture conditions for phosphate solubilization by a thermo-tolerant phosphate-solubilizing bacterium Brevibacillus sp. BISR-HY65 isolated from phosphate mines. Biocatal. Agric. Biotechnol., 2013, 2(3), 217–225.
  • Eurostat, Statistics Explained, 2015; http://ec.europa.eu/eurostat/statistics-explained/
  • Vazquez, P., Holguin, G., Puente, M., Lopez-Cortes, A. and Bashan, Y., Phosphate-solubilizing microorganisms associated with the rhizosphere of mangroves in a semiarid coastal lagoon. Biol. Fert. Soils, 2000, 30, 460–468.
  • Peix, A., Rivas, R., Mateos, P. F., Martínez-Molina, E., Rodríguez-Barrueco, C. and Velázquez, E., Pseudomonas rhizosphaerae sp. nov., a novel species that actively solubilizes phosphate in vitro. Int. J. Syst. Evol. Microbiol., 2003, 53, 2067–2072.
  • Seshadri, S., Muthukumarasamy, R., Lakshminarasimhan, R., Lakshiminarasimhan, C. and Ignacimuthu, S., Solubilization of inorganic phosphates by Azospirillum halopraeferans. Curr. Sci., 2000, 79, 565–567.
  • Son, H. J., Park, G. T., Cha, M. S. and Heo, M. S., Solubilization of insoluble inorganic phosphate by a novel salt- and pH-tolerant Pantoea agglomerans R-42 isolated from soybean rhizosphere. Biores. Technol., 2006, 97, 204–210.
  • Kumar, V., Aggarwal, N. K. and Singh, B. P., Performance and persistence of phosphate solubilizing Azotobacter chroococcumin wheat rhizosphere. Folia Microbiol., 2000, 45, 343–347.
  • Illmer, P. and Schinner, F., Solubilization of inorganic phosphates by phosphate by microorganisms isolated from forest soil. Soil Biol. Biochem., 1992, 24, 389–395.
  • Omar, S. A., The role of rock-phosphate-solubilizing fungi and vesicular-arbuscular–mycorrhiza (VAM) in growth of wheat plants fertilized with rock phosphate. World J. Microbiol. Biot., 1998, 14, 211–218.
  • Narula, N., Kumar, V., Behl, R. K., Duebel, A. A., Gransee, A. and Merbach, W., Effect of P solubilizing Azotobacter chroococcum on N, P, K uptake in P responsive wheat genotypes grown under green house conditions. J. Plant Nutr. Soil Sci., 2000, 163, 393–398.
  • Whitelaw, M. A., Growth promotion of plants inoculated with phosphate solubilizing fungi. Adv. Agron., 2000, 69, 99–151.
  • Ali Shah, F., Mahmood, Q., Shah, M. M., Pervez, A. and Asad, S. A., Microbial ecology of anaerobic digesters: the key players of anaerobiosis. Sci. World J., 2014, Article ID 183752.
  • Lidder, P. and Sonnino, A., Chapter 1 – biotechnologies for the management of genetic resources for food and agriculture. In Advances in Genetics, 2012, pp. 1–167.
  • Querido, J. F. B., Agirre, J., Marti, G. A., Guérin, D. M. A. and Silva, M. S., Molecular techniques for dicistrovirus detection without RNA extraction or purification. BioMed Res. Int., 2013, Article ID 218593.
  • Kim, K. Y. M., Ko, M. H. and Liu, H., Phylogenetic relationships of Pseudorasbora, Pseudopungtungia, and Pungtungia (Teleostei; Cypriniformes; Gobioninae) inferred from multiple nuclear gene sequences. BioMed Res. Int., 2013, Article ID 347242.
  • Acevedo, E., Galindo-Castañeda, T., Prada, F., Navia, M. and Romero, H. M., Phosphate-solubilizing microorganisms associated with the rhizosphere of oil palm (Elaeisguineensis Jacq.) in Colombia. Appl. Soil Ecol., 2014, 80, 26–33.
  • Jagielski, T., Van Ingen, J., Rastogi, N., Dziadek, J., Mazur, P. K. and Bielecki, J., Current methods in the molecular typing of Mycobacterium tuberculosis and other Mycobacteria. BioMed Res. Int., 2014, Article ID 645802.
  • Tripathi, D. P., Singh, G. and Panigrahi, D. C., Assessment of soil quality in the Jharia coalfield. Proceedings of the Seventh National Symposium on Environment, ISM, Dhanbad, 1998, p. 205.
  • Marmur, J. A., A procedure for the isolation of deoxyribonucleic acid from microorganisms. J. Mol. Biol., 1961, 3, 208–218.
  • Parani, K. and Saha, B. K., Prospects of using phosphate solubilizing pseudomonas as bio fertilizer. Eur. J. Biol. Sci., 2012, 4(2), 40–44.
  • Tahir, M., SajjadMirza, M., Zaheer, A., Dimitrov, M. R., Smidt, H. and Hameed, S., Isolation and identification of phosphate solubilizer Azospirillum, Bacillus and Enterobacter strains by
  • S rRNA sequence analysis and their effect on growth of wheat (Triticum aestivum L.). AJCS, 2013, 7(9), 1284–1292.
  • Volmer, J., Neumann, C., Bühler, B. and Schmid, A., Engineering of Pseudomonas taiwanensis VLB120 for constitutive solvent tolerance and increased specific styrene epoxidation activity. Appl. Environ. Microbiol., 2014, 80(20), 6539–6548.
  • Jaeger, K. E., Kharazmi, A. and Hoiby, N., Extracellular lipase of Pseudomonas aeruginosa: Biochemical characterization and effect of human neutrophil and monocyte function in vitro. Microbial Pathogen., 1991, 10, 173–182.
  • Prasad, M. P., Optimisation of fermentation conditions of phosphate solubilising bacteria – a potential biofertilizer. Int. J. Biol. Pharm. Allied Sci., 2014, 3(3), 406–414.
  • Priyanka, S., Shivaji, M. and Sridar, R., Isolation and charaterization of a novel multifunctional sulphur oxidizing bacterium (SOB) and its use as biofertilizer. Intl. Sci. J., 2014, 1, 28–34.
  • Eliya, M., Nisa, R. M. and Tjahjoleksono, A., Selection and identification of phosphate-potassium solubilizing bacteria from the area around the limestone mining in Cirebon Quarry. Res. J. Microbiol., 2015, 10, 270–279.

Abstract Views: 200

PDF Views: 73




  • Isolation and Characterization of Phosphorus Solubilizing Bacteria from Manganese Mining Area of Balaghat and Chhindwara

Abstract Views: 200  |  PDF Views: 73

Authors

Shikha Dixit
Department of Biological Sciences and Engineering, Maulana Azad National Institute of Technology, Bhopal 462 003, India
K. K. Appu Kuttan
Department of Mechanical Engineering, National Institute of Technology Suratkal, Suratkal 575 025, India
Rahul Shrivastava
Department of Biological Sciences and Engineering, Maulana Azad National Institute of Technology, Bhopal 462 003, India

Abstract


Plants require optimum amount of available phosphorus to support their growth and development. Phosphorus is known to have significant role in ischolar_main subdivision, vitality and disease resistance of plants. Different types of bacteria involved in phosphorus solubilization can be used as biofertilizer in reclamation of mining area. The present study deals with isolation and identification of phosphorus solubilizing bacteria from the manganese mining area of Balaghat and Chhindwara districts of Madhya Pradesh, India. rDNA (16s) based molecular identification was performed assisted by MEGA phylogenetic analysis. Pseudomonas putida, Bacillus licheniformis, Pseudomonas taiwanensis and Pseudomonas aeruginosa were explored as potential phosphorus solubilizers from the selected sites.

Keywords


Mining Area, Phosphorus Solubilizing Bacteria, 16s rDNA.

References





DOI: https://doi.org/10.18520/cs%2Fv113%2Fi03%2F500-504