Open Access Open Access  Restricted Access Subscription Access

DNA Forensics in Combating Food Frauds:A Study from China in Identifying Canned Meat Labelled as Deer Origin


Affiliations
1 State Key Laboratory of Genetic Resources and Evolution and Yunnan Laboratory of Molecular Biology of Domestic Animals, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, Yunnan, China
2 Guangzhou Institute of Pediatrics, Guangzhou Women and Children’s Medical Center, Guangzhou 510623, Guangdong, India
3 Wildlife Institute of India, Dehradun 248 001, India
4 Gujarat Forensic Sciences University, Gandhinagar 382 007, India
5 University School of Environment Management, Guru Gobind Singh Indraprastha University, Delhi 110 078, India
6 Allahabad High Court, Allahabad 211 001, India
 

Now-a-days processed and canned food products are consumed in increasing quantity in all developing and developed countries following changes in human lifestyle. However, customers are often fooled by products they buy and such frauds frequently go unreported since species identification requires technical inputs and considerable time and efforts. We bought two canned meat packets labelled as 'classic delicious deer meat' from Shanghai Pudong International Airport, Shanghai (People's Republic of China) to understand its origin as deer meat is legally prohibited in many parts of the world. In this study, we screened these samples with DNA barcoding approach using conserved mitochondrial genes. Homology search on NCBI and phylogenetic analysis identified these samples to have originated from a domestic pig of China. We propose that the methodology used is appropriate for identifying the processed and canned food products and further suggest to check the labelling regulations to guarantee the protection of consumers' rights.

Keywords

Canned Food, DNA Forensics, Food Frauds, Mitochondrial Genes, Species Identification.
User
Notifications
Font Size

  • Kane, D. E. and Hellberg, R. S., Identification of species in ground meat products sold on the US commercial market using DNA-based methods. Food Control, 2015, 59, 158–163.
  • Ballin, N. Z., Authentication of meat and meat products. Meat Sci., 2010, 86(3), 577–587.
  • Woolfe, M. and Primrose, S., Food forensics: using DNA technology to combat misdescription and fraud. Trends Biotechnol., 2004, 22(5), 222–226.
  • Teletchea, F., Maudet, C. and Hänni, C., Food and forensic molecular identification: update and challenges. Trends Biotechnol., 2005, 23, 359–366.
  • Meyer, R., Höfelein, C., Lüthy, J. and Candrian, U., Polymerase chain reaction–restriction fragment length polymorphism analysis: a simple method for species identification in food. J. AOAC Int., 1995, 78, 1542–1551.
  • Unseld, M., Beyermann, B., Brandt, P. and Hiesel, R., Identification of the species origin of highly processed meat products by mitochondrial DNA sequences. PCR Methods Appl., 1995, 4, 241–243.
  • Matsunaga, T., Chikuni, K., Tanabe, R., Muroya, S., Shibata, K., Yamada, J. and Shinmura, Y., A quick and simple method for the identification of meat species and meat products by PCR assay. Meat Sci., 1999, 51, 143–148.
  • Arahishi, F., PCR-RFLP analysis of nuclear nontranscribed spacer for mackerel species identification. J. Agric. Food Chem., 2005, 53, 508–511.
  • Wong, E. and Hanner, R., DNA barcoding detects market substitution in North American seafood. Food Res. Int., 2008, 41, 828–837.
  • Hellberg, R. S. and Morrissey, M. T., Advances in DNA-based techniques for the detection of seafood species substitution on the commercial market. J. Lab. Autom., 2011, 16, 308–321.
  • Shokralla, S., Hellberg, R. S., Handy, S. M., King, I. and Hajibabaei, M. A., DNA mini-barcoding system for authentication of processed fish products. Sci. Rep., 2015, 5, 15894; http://doi.org/10.1038/srep15894.
  • McCullough, D. L., Jiang, Z. G. and Li, C. W. (eds), In Sika Deer: Biology and Management of Native and Introduced Populations, Springer, Tokyo, New York, 2009, pp. 521–539; doi:10.1007/978-4-431-09429-6_35.
  • Harris, R. B., Cervus nippon. The IUCN Red List of Threatened Species, Version 2015.2, 2015; www.iucnredlist.org (accessed on 28 August 2015).
  • Verma, S. K. and Singh, L., Novel universal primers establish identify of an enormous number of animal species for forensic application. Mol. Ecol. Notes, 2003, 3, 28–31.
  • Mitchell, S. E., Cockburn, A. F. and Seawright, J. A., The mitochondrial genome of Anopheles quadrimaculatus species A: complete nucleotide and organization. Genome, 1993, 36, 1058–1073.
  • Kocher, T. D. et al., Dynamics of mitochondrial DNA evolution in animals: amplification and sequencing with conserved primers. Proc. Natl. Acad. Sci. USA, 1989, 86, 6196–6200.
  • Hall, T. A., BioEdit: a user-friendly biological sequence alignment editor and analysis program for windows 95/98/NT. Nucleic Acids Symp. Ser., 1999, 41, 95–98.
  • Tamura, K. et al., MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol. Biol. Evol., 2011, 28, 2731–2739.
  • Bartlett, S. E. and Davidson, W. S., FINS (forensically informative nucleotide sequencing): a procedure for identifying the animal origin of biological specimens. Biotechniques, 1992, 12, 408–411.
  • Guha, S. and Kashyap, V. K., Molecular identification of lizard by RAPD and FINS of mitochondrial 16s rRNA gene. Legal Med. Tokyo, 2006, 8, 5–10.
  • Sahajpal, V. and Goyal, S. P., Identification of a forensic case using microscopy and forensically informative nucleotide sequencing (FINS): A case study of small Indian civet (Viverricula indica). Sci. Justice, 2010, 50, 94–97.
  • Mukesh, Singh, S. K., Shukla, M., Sharma, L. K., Mohan, N., Goyal, S. P. and Sathyakumar, S., Identification of Galliformes through forensically informative nucleotide sequencing (FINS) and its implication in wildlife forensics. J. Forensic Res., 2013, 4, 195; doi:10.4172/2157-7145.1000195.
  • Hillis, D. M. and Bull, J. J., An empirical test of bootstrapping as a method for assessing confidence in phylogenetic analysis. Syst. Biol., 1993, 42, 182–192.

Abstract Views: 321

PDF Views: 86




  • DNA Forensics in Combating Food Frauds:A Study from China in Identifying Canned Meat Labelled as Deer Origin

Abstract Views: 321  |  PDF Views: 86

Authors

Mukesh Thakur
State Key Laboratory of Genetic Resources and Evolution and Yunnan Laboratory of Molecular Biology of Domestic Animals, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, Yunnan, China
Ruheena Javed
Guangzhou Institute of Pediatrics, Guangzhou Women and Children’s Medical Center, Guangzhou 510623, Guangdong, India
Ved Prakash Kumar
Wildlife Institute of India, Dehradun 248 001, India
Malay Shukla
Gujarat Forensic Sciences University, Gandhinagar 382 007, India
Neha Singh
University School of Environment Management, Guru Gobind Singh Indraprastha University, Delhi 110 078, India
Aishwarya Maheshwari
Wildlife Institute of India, Dehradun 248 001, India
Nipun Mohan
Allahabad High Court, Allahabad 211 001, India
Dong-Dong Wu
State Key Laboratory of Genetic Resources and Evolution and Yunnan Laboratory of Molecular Biology of Domestic Animals, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, Yunnan, China
Ya-Ping Zhang
State Key Laboratory of Genetic Resources and Evolution and Yunnan Laboratory of Molecular Biology of Domestic Animals, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, Yunnan, China

Abstract


Now-a-days processed and canned food products are consumed in increasing quantity in all developing and developed countries following changes in human lifestyle. However, customers are often fooled by products they buy and such frauds frequently go unreported since species identification requires technical inputs and considerable time and efforts. We bought two canned meat packets labelled as 'classic delicious deer meat' from Shanghai Pudong International Airport, Shanghai (People's Republic of China) to understand its origin as deer meat is legally prohibited in many parts of the world. In this study, we screened these samples with DNA barcoding approach using conserved mitochondrial genes. Homology search on NCBI and phylogenetic analysis identified these samples to have originated from a domestic pig of China. We propose that the methodology used is appropriate for identifying the processed and canned food products and further suggest to check the labelling regulations to guarantee the protection of consumers' rights.

Keywords


Canned Food, DNA Forensics, Food Frauds, Mitochondrial Genes, Species Identification.

References





DOI: https://doi.org/10.18520/cs%2Fv112%2Fi12%2F2449-2452