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Cultural Endophytic Fungi Associated with Dendrobium Officinale:Identification, Diversity Estimation and their Antimicrobial Potential


Affiliations
1 School of Biological Engineering, Dalian Polytechnic University, #1 Qinggongyuan, Ganjingzi District, Dalian, Liaoning, 116034, P.R., China
2 Department of R&D, Dalian Hissen Bio-Pharmaceutical Co., Ltd., Dalian, 116600, China
 

Endophytic fungi associated with Chinese medicinal plant Dendrobium officinale have been revealed using the culture-dependent approach and the diversity and antimicrobial activity have been examined in this study. A total of 105 endophytic isolates representing 16 fungal morphotypes were recovered from the ischolar_mains, stems and leaves of D. officinale. Identification by ITS-rDNA sequencing showed 12 distinct operational taxonomic units, and most of them were classified into species level. Shannon's and Simpson's indices were calculated to quantify fungal diversity. The results demonstrated that endophytic fungi associated with D. officinale were moderately diverse, and fungal species among three tissues was also different which exhibited tissue-specificity. The composition of endophyte assemblages from the ischolar_mains was most abundant and diverse, followed by the stems, while the leaves possessed relatively low isolation rate. Fusarium oxysporum (21.0%) and Xylaria cubensis (20.0%) were the most dominant species, while Colletotrichum sp. and Pestalotiopsis sp. were also frequently isolated. Three species (F. oxysporum, Aspergillus niger and Aureobasidium pullulan) showed broad-spectrum antimicrobial activity, and F. oxysporum had acute inhibition to pathogens tested. Our systematic study could enrich the current knowledge on diversity of endophytic fungi. The fungal isolates from D. officinale may be used for exploration of novel bioactive compounds and have potential as biocontrol agents.

Keywords

Antimicrobial Activity, Dendrobium officinale, Diversity, Endophytic Fungi.
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  • Yu, J., Zhou, X. F., Yang, S. J., Liu, W. H. and Hu, X. F., Design and application of specific 16S rDNA-targeted primers for assessing endophytic diversity in Dendrobium officinale using nested PCR-DGGE. Appl. Microbiol. Biotechnol., 2013, 97, 9825–9836.

  • Pei, C., Mi, C. Y., Sun, L. H., Liu, W. H., Li, O. and Hu, X. F., Diversity of endophytic bacteria of Dendrobium officinale based on culture-dependent and culture-independent methods. Biotechnol. Biotechnol. Equip., 2016, 11, 1–8.

  • Yang, S. J., Zhang, X. H., Cao, Z. Y., Zhao, K. P., Wang, S., Chen, M. X. and Hu, X. F., Growth-promoting Sphingomonas paucimobilis ZJSH1 associated with Dendrobium officinale through phytohormone production and nitrogen fixation. Microb. Biotechnol., 2014, 7, 611–620.

  • Ganley, R. J., Brunsfeld, S. J. and Newcombe, G., A community of unknown endophytic fungi in western white pine. Proc. Natl. Acad. Sci. USA, 2004, 101, 10107–10112.

  • Arnold, A. E., Maynard, Z., Gilbert, G. S., Coley, P. D. and Kursar, T. A., Are tropical fungal endophytes hyperdiverse? Ecol. Lett., 2000, 3, 267–274.

  • Rodriguez, R. J., White Jr, J. F., Arnold, A. E. and Redman, R. S., Fungal endophytes: diversity and functional roles. New Phytol., 2009, 182, 314–330.

  • Verma, V. C., Gond, S. K., Kumar, A., Mishra, A., Kharwar, R. N. and Gange, A. C., Endophytic actinomycetes from Azadirachta indica A Juss: isolation, diversity, and anti-microbial activity. Microb. Ecol., 2009, 57, 749–756.

  • Aly, A. H., Debbab, A., Kjer, J. and Proksch, P., Fungal endophytes from higher plants: a prolific source of phytochemicals and other bioactive natural products. Fungal Divers., 2010, 41, 1–16.

  • Priti, V., Ramesha, B. T., Shweta Singh, Ravikanth, G., Ganeshaiah, K. N., Suryanarayanan, T. S. and Uma Shaanker, R., How promising are endophytic fungi as alternative sources of plant secondary metabolites. Curr. Sci., 2009, 97, 477–478.

  • Tan, R. X. and Zou, W. X., Endophytes: a rich source of functional metabolites. Nat. Prod. Rep., 2001, 18, 448–459.

  • Arnold, A. E. et al., Fungal endophytes limit pathogen damage in a tropical tree. Proc. Natl. Acad. Sci. USA, 2003, 100, 15649– 15654.

  • Radic, N. and Štrukelj, B., Endophytic fungi – the treasure chest of antibacterial substances. Phytomedicine, 2012, 19, 1270– 1284.

  • Kharwar, R. N., Mishra, A., Gond, S. K., Stierle, A. and Stierle, D., Anticancer compounds derived from fungal endophytes: their importance and future challenges. Nat. Prod. Rep., 2011, 28, 1208–1228.

  • Xing, Y. M., Chen, J., Cui, J. L., Chen, X. M. and Guo, S. X., Antimicrobial activity and biodiversity of endophytic fungi in Dendrobium devonianum and Dendrobium thyrsiflorum from Vietman. Curr. Microbiol., 2011, 62, 1218–1224.

  • Yuan, Z. L., Zhang, C. L., Lin, F. C. and Kubicek, C. P., Identity, diversity, and molecular phylogeny of the endophytic mycobiota in the ischolar_mains of rare wild rice (Oryza granulate) from a nature reserve in Yunnan, China. Appl. Environ. Microbiol., 2010, 76, 1642–1652.

  • De Souza Sebastianes, F. L., Romao-Dumaresq, A. S., Lacava, P. T., Harakava, R., Azevedo, J. L., de Melo, I. S. and PizziraniKleiner, A. A., Species diversity of culturable endophytic fungi from Brazilian mangrove forests. Curr. Genet., 2013, 59, 153– 166.

  • Jin, Z. X., Guo, Q., Zhang, Z. S. and Yan, T. S., Biodegradation of type II pyrethroids and major degraded products by a newly isolated Acinetobacter sp. strain JN8. Can. J. Microbiol., 2014, 60, 541–545.

  • Huang, W. Y., Cai, Y. Z., Hyde, K. D., Corke, H. and Sun, M., Biodiversity of endophytic fungi associated with 29 traditional Chinese medicinal plants. Fungal Divers., 2008, 33, 61–75.

  • Kusari, P., Kusari, S., Spiteller, M. and Kayser, O., Endophytic fungi harbored in Cannabis sativa L.: diversity and potential as biocontrol agents against host plant-specific phytopathogens. Fungal Divers., 2013, 60, 137–151.

  • Gazis, R. and Chaverri, P., Diversity of fungal endophytes in leaves and stems of wild rubber trees (Hevea brasiliensis) in Peru. Fungal Ecol., 2010, 3, 240–254.

  • Wang, Y., Lu, Z. X., Wu, H. and Lv, F. X., Study on the antibiotic activity of microcapsule curcumin against foodborne pathogens. Int. J. Food Microbiol., 2009, 136, 71–74.

  • Rivera-Orduña, F. N., Suarez-Sanchez, R. A., Flores-Bustamante, Z. R., Gracida-Rodriguez, J. N. and Flores-Cotera, L. B., Diversity of endophytic fungi of Taxus globosa (Mexican yew). Fungal Divers., 2011, 47, 65–74.

  • Chen, J., Hu, K. X., Hou, X. Q. and Guo, S. X., Endophytic fungi assemblages from 10 Dendrobium medicinal plants (Orchidaceae). World J. Microbiol. Biotechnol., 2011, 27, 1009–1016.

  • Chen, J., Wang, H. and Guo, S. X., Isolation and identification of endophytic and mycorrhizal fungi from seeds and ischolar_mains of Dendrobium (Orchidaceae). Mycorrhiza, 2012, 22, 97–307.

  • Chen, J. et al., Diversity and taxonomy of endophytic Xylariaceous fungi from medicinal plants of Dendrobium (Orchidaceae). PLoS ONE, 2013, 8, 1–11.

  • Chen, X. M., Dong, H. L., Hu, K. X., Sun, Z. R., Chen, J. and Guo, S. X., Diversity and antimicrobial and plant-growthpromoting activities of endophytic fungi in Dendrobium loddigesii Rolfe. J. Plant Growth Regul., 2010, 29, 328–337.

  • Yuan, Z. L., Chen, Y. C. and Yang, Y., Diverse non-mycorrhizal fungal endophytes inhabiting an epiphytic, medicinal orchid (Dendrobium nobile): estimation and characterization. World J. Microb. Biotechnol., 2009, 25, 295–303.

  • Cui, J., Wang, Y., Xing, Y., Guo, S., Xiao, P. and Wang, M. L., Antimicrobial activity of endophytic fungi isolated from Dendrobium species in southwestern China. China J. Chin. Mater. Med., 2012, 37, 764–770.

  • Ghimire, S. R., Charlton, N. D., Bell, J. D., Krishnamurthy, Y. L. and Craven, K. D., Biodiversity of fungal endophyte communities inhabiting switch grass (Panicum virgatum L.) growing in the native tall grass prairie of northern Oklahoma. Fungal Divers., 2011, 47, 19–27.

  • Miller, K. I., Qing, C., Sze, D. M. Y., Roufogalis, B. D. and Neilan, B. A., Culturable endophytes of medicinal plants and the genetic basis for their bioactivity. Microb. Ecol., 2012, 64, 431– 449.

  • Strobel, G. and Daisy, B., Bioprospecting for microbial endophytes and their natural products. Microbiol. Mol. Biol. Rev., 2003, 67, 491–502.

  • Crozier, J., Thomas, S. E., Aime, M. C., Evans, H. C. and Holmesa, K. A., Molecular characterization of fungal endophytic morphospecies isolated from stems and pods of Theobroma cacao. Plant Pathol., 2006, 55, 783–791.

  • Liu, X. L., Dong, M. S., Chen, X. H., Jiang, M., Lv, X. and Zhou, J. Z., Antimicrobial activity of an endophytic Xylaria sp. YX-28 and identification of its antimicrobial compound 7-amino-4methylcoumarin. Appl. Microbiol. Biotechnol., 2008, 78, 241– 247.

  • Kumar, D. S. S. and Hyde, K. D., Biodiversity and tissuerecurrence of endophytic fungi in Tripterygium wilfordii. Fungal Divers., 2004, 17, 69–90.

  • Manamgoda, D. S., Udayanga, D., Cai, L., Chukeatirote, E. and Hyde, K. D., Endophytic Colletotrichum from tropical grasses with a new species C. endophytica. Fungal Divers., 2013, 61, 107–115.


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  • Cultural Endophytic Fungi Associated with Dendrobium Officinale:Identification, Diversity Estimation and their Antimicrobial Potential

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Authors

Zhaoxia Jin
School of Biological Engineering, Dalian Polytechnic University, #1 Qinggongyuan, Ganjingzi District, Dalian, Liaoning, 116034, P.R., China
Dandan Li
School of Biological Engineering, Dalian Polytechnic University, #1 Qinggongyuan, Ganjingzi District, Dalian, Liaoning, 116034, P.R., China
Tianyi Liu
School of Biological Engineering, Dalian Polytechnic University, #1 Qinggongyuan, Ganjingzi District, Dalian, Liaoning, 116034, P.R., China
Fang Yu
School of Biological Engineering, Dalian Polytechnic University, #1 Qinggongyuan, Ganjingzi District, Dalian, Liaoning, 116034, P.R., China
Zongshen Zhang
School of Biological Engineering, Dalian Polytechnic University, #1 Qinggongyuan, Ganjingzi District, Dalian, Liaoning, 116034, P.R., China
Caixia Su
Department of R&D, Dalian Hissen Bio-Pharmaceutical Co., Ltd., Dalian, 116600, China
Yanyan Wang
School of Biological Engineering, Dalian Polytechnic University, #1 Qinggongyuan, Ganjingzi District, Dalian, Liaoning, 116034, P.R., China
Qiong Guo
School of Biological Engineering, Dalian Polytechnic University, #1 Qinggongyuan, Ganjingzi District, Dalian, Liaoning, 116034, P.R., China
Zhiwen Liu
School of Biological Engineering, Dalian Polytechnic University, #1 Qinggongyuan, Ganjingzi District, Dalian, Liaoning, 116034, P.R., China

Abstract


Endophytic fungi associated with Chinese medicinal plant Dendrobium officinale have been revealed using the culture-dependent approach and the diversity and antimicrobial activity have been examined in this study. A total of 105 endophytic isolates representing 16 fungal morphotypes were recovered from the ischolar_mains, stems and leaves of D. officinale. Identification by ITS-rDNA sequencing showed 12 distinct operational taxonomic units, and most of them were classified into species level. Shannon's and Simpson's indices were calculated to quantify fungal diversity. The results demonstrated that endophytic fungi associated with D. officinale were moderately diverse, and fungal species among three tissues was also different which exhibited tissue-specificity. The composition of endophyte assemblages from the ischolar_mains was most abundant and diverse, followed by the stems, while the leaves possessed relatively low isolation rate. Fusarium oxysporum (21.0%) and Xylaria cubensis (20.0%) were the most dominant species, while Colletotrichum sp. and Pestalotiopsis sp. were also frequently isolated. Three species (F. oxysporum, Aspergillus niger and Aureobasidium pullulan) showed broad-spectrum antimicrobial activity, and F. oxysporum had acute inhibition to pathogens tested. Our systematic study could enrich the current knowledge on diversity of endophytic fungi. The fungal isolates from D. officinale may be used for exploration of novel bioactive compounds and have potential as biocontrol agents.

Keywords


Antimicrobial Activity, Dendrobium officinale, Diversity, Endophytic Fungi.

References





DOI: https://doi.org/10.18520/cs%2Fv112%2Fi08%2F1690-1697