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Antiproliferative Effects of Fluorine Substitute 3,5-di-tert-butylphenol Bearing Schiff Bases Using CFSE-Based Cell Proliferation Assay


Affiliations
1 Department of Immunology, Istanbul University, Istanbul, Turkey
2 Department of Medical Pharmacology, Istanbul University, Istanbul, Turkey
3 Department of Diabetes Application and Research Center, Istanbul University, Istanbul, Turkey
 

The determination of antiproliferative properties of compounds on tumour cells is important for assessment of their efficacy in cancer treatment. CFSE labelled K562 cells were incubated with doxorubicin and ortho- or para-fluorosubstitute Schiff bases (compounds 1 and 2 respectively). CFSE intensities were analysed using flow cytometry. K562 cells treated with doxorubicin resulted in homogeneous high intensity fluorescence after 96 h of incubation. Schiff bases exhibited antiproliferative effects, but lower than doxorubicin. Our results reveal that CFSE assay can be used for determining in vitro anti proliferative features of anticancer drugs and/or compounds from herbal or chemical sources.

Keywords

Cancer, CFSE, Doxorubicin.
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  • Antiproliferative Effects of Fluorine Substitute 3,5-di-tert-butylphenol Bearing Schiff Bases Using CFSE-Based Cell Proliferation Assay

Abstract Views: 220  |  PDF Views: 65

Authors

Ali Osman Gurol
Department of Immunology, Istanbul University, Istanbul, Turkey
Veli Kasim
Department of Medical Pharmacology, Istanbul University, Istanbul, Turkey
Faruk Suzergoz
Department of Diabetes Application and Research Center, Istanbul University, Istanbul, Turkey

Abstract


The determination of antiproliferative properties of compounds on tumour cells is important for assessment of their efficacy in cancer treatment. CFSE labelled K562 cells were incubated with doxorubicin and ortho- or para-fluorosubstitute Schiff bases (compounds 1 and 2 respectively). CFSE intensities were analysed using flow cytometry. K562 cells treated with doxorubicin resulted in homogeneous high intensity fluorescence after 96 h of incubation. Schiff bases exhibited antiproliferative effects, but lower than doxorubicin. Our results reveal that CFSE assay can be used for determining in vitro anti proliferative features of anticancer drugs and/or compounds from herbal or chemical sources.

Keywords


Cancer, CFSE, Doxorubicin.



DOI: https://doi.org/10.18520/cs%2Fv112%2Fi03%2F619-624