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Vertebrate-Type Steroid Profile in Different Tissues of Wild and Endocrinologically Manipulated Female Brood Stocks of Penaeus monodon


Affiliations
1 Central Institute of Brackishwater Aquaculture, 75 Santhome High Road, RA Puram, Chennai 600 028, India
2 Centre for Climate Change Studies, Sathyabama University, Chennai 600 119, India, India
 

The endocrine mechanism regulating reproduction of tiger shrimp, Penaeus monodon, is a field of topical interest, which is to be addressed in detail as control of reproduction in captivity is conditional for domestication. In this study, the titres of estradiol and progesterone in hemolymph, ovary and hepatopancreas during various reproductive phases of females were quantified using ELISA. Circulating concentration of hemolymph estradiol and progesterone in the endocrinologically-induced (eyestalk ablated) females was also quantified. Additionally, the evidence for estradiol and progesterone receptors was provided using immunohistochemistry. Levels of progesterone in hemolymph and ovary were highest in the vitellogenic and ripe phases (P > 0.05). Conversely, in hepatopancreas significantly higher progesterone level was detected at immature phase (184.3 ± 2.3 pg/ml). Estradiol was highest in hemolymph (65.97 ± 0.8 pg/ml) and ovary (58.47 ± 0.7 pg/ml) at the early vitellogenic phase. In hepatopancreas, however, lowest level of estradiol was detected at the early vitellogenic phase (121 ± 0.38 pg/ml). Progesterone and estradiol receptor protein signals were predominantly found in the pre vitellogenic oocytes. These results suggest the possible involvement of vertebrate-type steroid in the reproductive maturation of P. monodon.

Keywords

Eyestalk Ablation, Immunohistochemistry, Penaeus monodon, Reproductive Maturation, Sex Steroids.
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  • Vertebrate-Type Steroid Profile in Different Tissues of Wild and Endocrinologically Manipulated Female Brood Stocks of Penaeus monodon

Abstract Views: 296  |  PDF Views: 84

Authors

J. R. Merlin
Central Institute of Brackishwater Aquaculture, 75 Santhome High Road, RA Puram, Chennai 600 028, India
D. L. Mohanlal
Central Institute of Brackishwater Aquaculture, 75 Santhome High Road, RA Puram, Chennai 600 028, India
C. P. Balasubramanian
Central Institute of Brackishwater Aquaculture, 75 Santhome High Road, RA Puram, Chennai 600 028, India
K. K. Vijayan
Central Institute of Brackishwater Aquaculture, 75 Santhome High Road, RA Puram, Chennai 600 028, India
T. Subramoniam
Centre for Climate Change Studies, Sathyabama University, Chennai 600 119, India, India
Sherly Tomy
Central Institute of Brackishwater Aquaculture, 75 Santhome High Road, RA Puram, Chennai 600 028, India
J. Syama Dayal
Central Institute of Brackishwater Aquaculture, 75 Santhome High Road, RA Puram, Chennai 600 028, India
C. Gopal
Central Institute of Brackishwater Aquaculture, 75 Santhome High Road, RA Puram, Chennai 600 028, India

Abstract


The endocrine mechanism regulating reproduction of tiger shrimp, Penaeus monodon, is a field of topical interest, which is to be addressed in detail as control of reproduction in captivity is conditional for domestication. In this study, the titres of estradiol and progesterone in hemolymph, ovary and hepatopancreas during various reproductive phases of females were quantified using ELISA. Circulating concentration of hemolymph estradiol and progesterone in the endocrinologically-induced (eyestalk ablated) females was also quantified. Additionally, the evidence for estradiol and progesterone receptors was provided using immunohistochemistry. Levels of progesterone in hemolymph and ovary were highest in the vitellogenic and ripe phases (P > 0.05). Conversely, in hepatopancreas significantly higher progesterone level was detected at immature phase (184.3 ± 2.3 pg/ml). Estradiol was highest in hemolymph (65.97 ± 0.8 pg/ml) and ovary (58.47 ± 0.7 pg/ml) at the early vitellogenic phase. In hepatopancreas, however, lowest level of estradiol was detected at the early vitellogenic phase (121 ± 0.38 pg/ml). Progesterone and estradiol receptor protein signals were predominantly found in the pre vitellogenic oocytes. These results suggest the possible involvement of vertebrate-type steroid in the reproductive maturation of P. monodon.

Keywords


Eyestalk Ablation, Immunohistochemistry, Penaeus monodon, Reproductive Maturation, Sex Steroids.

References





DOI: https://doi.org/10.18520/cs%2Fv111%2Fi7%2F1194-1200