Asian Journal of Research in Pharmaceutical Sciences

Bhalerao Atharva ¹, Gade Vilas ¹, Sonawane Sandeep ¹, Chhajed Santosh ², Kshirsagar Sanjay ¹

Affiliations
1 Department of Quality Assurance Techniques, MET’s Institute of Pharmacy, MET League of Colleges, Bhujbal Knowledge City, Adgaon, Nashik 422 003, Maharashtra State, IN
2 Department of Pharmaceutical Chemistry, MET’s Institute of Pharmacy, MET League of Colleges, Bhujbal Knowledge City, Adgaon, Nashik 422 003, Maharashtra State, IN

Abstract

A simple, rapid and accurate RP-HPLC method was developed and validated for the estimation of cefuroxime axetil in spiked human plasma using liquid-liquid extraction. Sufficient recovery was obtained when drug and internal standard (Guaifensin) were extracted using ethyl acetate with 1% formic acid. The chromatographic separation was performed on C18 (250 × 4.6 mm, 5 μm) column with mobile phase consisting of Potassium dihydrogenortho phosphate: methanol (60:40% v/v). The flow rate was kept constant at 1 mL/min and detection was carried out at 276 nm. The calibration curve was found linear in the range of 1000-32000 ng/mL To minimize the heteroscedasticity, weighted calibration models were used with different weighing factors. The method was validated as per the US-FDA guidelines.

Keywords

Cefuroxime Axetil, Weighted Regression, RP-HPLC, Spiked Human Plasma, Bioanalytical Method Validation.

Full Text