Asian Journal of Research in Pharmaceutical Sciences

Pradeep Kumar Samal ¹

Affiliations
1 SLT Institute of Pharmaceutical Sciences, Guru Ghasidas Vishwavidyalaya, Bilaspur, Chhattisgarh, IN

Abstract

The objective of this study was to investigate the hepatoprotective activity of alcoholic extract of Ardisia solanacea leaves against carbon tetrachloride (CCL₄) induced hepatotoxicity. The plant material were dried in shade then powdered and extracted with alcohol. Preliminary phytochemical tests were done. Alcoholic extract showed presence of phenolic compound and flavanoids. The hepatoprotective activity of the alcoholic extract was assessed in CCL₄ induced hepatotoxic rats. Alteration in the levels of biochemical markers of hepatic damage like SGOT, SGPT, ALP, Billirubin and Protein were tested in both CCL₄ treated and untreated groups. CCL₄ (1ml) has enhanced the SGOT, SGPT, ALP and Total Billirubin where decrease in total protein level in liver. Treatment of alcoholic extract of Ardisia Solanacea (200mg/kg) has brought back the altered levels of biochemical markers to the near normal levels in the dose dependent manner. Our findings suggested that Ardisia Solanacea alcoholic leaf extract possessed hepatoprotective activity. Moreover, it prevented CCL₄induced prolongation in pentobarbital sleeping time confirming hepatoprotectivity and validates the traditional use of this plant against liver damage.

Keywords

Ardisia solanacea, Hepatoprotective, Silymarin, CCL₄, Alcohol.

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Pradeep Kumar Samal ¹

Affiliations
1 SLT Institute of Pharmaceutical Sciences, Guru Ghasidas Vishwavidyalaya, Bilaspur, Chhattisgarh, IN

Abstract

The objective of this study was to investigate the antioxidant activity of Hibiscus vitifolius leaves against carbon tetracholoride (CCl₄) induced hepatotoxic model. The plant materials were dried in shade, powdered with help of hand grinder and extracted with alcohol. Preliminary phytochemical test shows the presence of Alkaloids, Carbohydrates, Tannins, Amino acids and Flavanoids. The ethanolic extract of (EEHV) showed a significant dose dependent (250 mg and 500 mg/kg p.o.) antioxidant activity. The degree of antioxidant activity was measured by using serum biochemical parameters like hepatic antioxidant enzymes like SOD, CAT, GPX and MDA. EEHV at a dose level of 250mg/kg and 500mg/kg produce significant (P<0.001) antioxidant activity by decreasing the level Malondialdehyde, while it significantly increased the levels of Glutathione peroxidise (GPX), superoxide dismutase (SOD) and Catalase activity (CAT) in a dose dependent manner. The effect of EEHV was comparable to that of standard drug Silymarin.

Keywords

Hibiscus vitifolius, SOD, GPX, CAT, MDA.

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