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Rakesh, Sachin U.
- HPTLC Method for Quantitative Determination of Gallic Acid in Hydroalcoholic Extract of Dried Flowers of Nymphaea Stellata Willd
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1 Govt. College of Pharmacy, Vidyanagar, Tal. Karad- 415 124, Dist. Satara, IN
1 Govt. College of Pharmacy, Vidyanagar, Tal. Karad- 415 124, Dist. Satara, IN
Source
Asian Journal of Research in Chemistry, Vol 2, No 2 (2009), Pagination: 131-134Abstract
A sensitive and reliable high performance thin layer chromatographic method has been developed for quantitation of gallic acid in the dried flowers of Nymphaea stellata willd. The hydroalcoholic extract of N. stellata was chromatographed on silica gel 60 F254 plates with chloroform: ethyl acetate: formic acid, 7.5: 6: 0.5 (v/v/v), as mobile phase. Detection and quantitation were performed by densitrometric scanning at = 292 nm, by using deuterium lamp. The accuracy of the method was checked by conducting recovery studies at three different levels, using the standard addition method. The average recovery of gallic acid was found to be 98.33%. The proposed HPTLC method provide a good resolution of gallic acid from other constituents present in hydroalcoholic extract of dried flowers of N. stellata and can be used for quantitation of gallic acid present in extract. The method is rapid, simple and precise.Keywords
HPTLC, Gallic Acid, Nymphaea stellata Willd.- Simultaneous UV Spectrophotometric Method for Estimation of Losartan Potssium and Amlodipine Besylate in Tablet Dosage Form
Abstract Views :152 |
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Authors
Affiliations
1 Govt. College of Pharmacy, Karad- 415 124, (Satara), Maharashtra, IN
1 Govt. College of Pharmacy, Karad- 415 124, (Satara), Maharashtra, IN