Asian Journal of Research in Chemistry

R. Vijayalakshmi ¹, S. Bhargavi ¹, S. Archana ¹, M. D. Dhanaraju ¹

Affiliations
1 Research Lab, GIET School of Pharmacy, NH-5, Chaitanya Nagar, Rajahmundry-533294, IN

Abstract

Rapid, simple and sensitive spectrophotometric methods (A&B) have been developed for the determination of Atorvastatin (ATN) in pharmaceutical bulk and tablet dosage form. Method A is based on the reaction of ATN with 3-methyl-2-benzothiazolinone hydrazone and ferric chloride to form green colored chromogen. Method B is based on the formation of wine red colored chromogen with 1,2-naphthaquinone-4-sulphonate and NaOH. The absorbances of the chromogens were measured at their respective wavelengths of maximum absorbance against the corresponding reagent blank. The proposed methods have been successfully applied to the analysis of the bulk drug and its tablet dosage form. The methods have been statistically evaluated and were found to be precise and accurate.

Keywords

Atorvastatin, 3-Methyl-2-Benzothiazolinone Hydrazone, Ferric Chloride, 1, 2-Naphthaquinone-4-Sulphonate, Sodium Hydroxide.

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R. Vijayalakshmi ¹, S. Archana ¹, M. D. Dhanaraju ¹

Affiliations
1 Research Lab., GIET School of Pharmacy, NH-5 Chaitanya Nagar, Rajahmundry-533294, IN

Abstract

Perindopril and losartan are used in combination for treatment of hypertension. The present work deals with a simple spectrophotometric method development for simultaneous estimation of perindopril (PRL) and losartan (LSN) in two component-formulation. First order derivative spectrophotometric method is adopted to eliminate spectral interference. The method obeys beer’s law in the concentration ranges employed for evaluation. To determine the sampling wave length, 10 mcg/ml of PRL and LSN were scanned from 200-350 nm. The wavelengths were found to be 219 nm for PRL, where LSN showed zero crossing point and 214 nm for LSN, where PRL showed zero crossing point in First order derivative spectroscopy. The recovery studies confirmed accuracy of proposed method and low values of standard deviation confirmed precision of the method. The method is validated as per ICH guidelines.

Keywords

Perindopril, Losartan, First Order Derivative Spectroscopy.

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