Asian Journal of Research in Chemistry

R. Siva Kumar ¹, Shaik Nafeez Basha ¹, P. Kumar Nallasivan ¹, W. D. Sam Solomon ¹, R. Venkatnarayanan ¹

Affiliations
1 Dept. of Pharmaceutical Chemistry, RVS College of Pharmaceutical Sciences, Sulur, Coimbatore-641402, (T.N.), IN

Abstract

The Protein-Ligand interaction plays a significant role in structural based drug designing. The highly pathogenic influenza A virus subtype H5N1 virus is an emerging avian influenza virus that has been causing global concern as a potential pandemic threat. It is often referred to simply as "bird flu" or "avian influenza". In our research work we have taken influenza A virus H5N1 receptor. The receptor was docked to the commercially available drugs zanamivir and oseltamivir and the energy value obtained are as follows; zanamivir (-231.74) and oseltamivir (-243.74) using the HEX docking software. We tried to improve the binding efficiency and steric compatibility of zanamivir against N5N1 receptor. Several modifications were made to the probable functional groups which were interacting with the receptor molecule. Analogs of this drug molecule were prepared using ACD ChemSketch and docked using HeX docking software. Zanamivir analog 3 and oseltamivir analog 5 were detected with significant energy values and probable lead molecules. The Modified drugs was sketched using Chemsketch were found to be better than the conventional drugs available. Further from this work we can improve the steric compatibility and then ADME properties of the Analogs can be analyzed using Inslico ADME tools available.

Keywords

Bird Flu, Chemsketch, Docking, Hex, Rasmol.

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R. Siva Kumar ¹, P. Kumar Nallasivan ¹, S. Saravanakumar ¹, C. S. Kandasamy ¹, R. Venkatnarayanan ¹

Affiliations
1 Dept. of Pharmaceutical Analysis, RVS College of Pharmaceutical Sciences, Sulur, Coimbatore-641402, Tamilnadu, IN

Abstract

Reverse Phase HPLC method for the determination of nitazoxanide and ofloxacin in bulk and tablet formulations. The determination was carried out by using Phenomenex C18 column with 0.24% sodium lauryl sulphate:acetonitrile:acetic acid (pH-4.0) (58:40:02) as the mobile phase. The flow rate was 1.5 ml/min and the eluent was monitored at 295 nm. The Retention time of nitazoxanide and ofloxacin were 2.2 and 5.4 respectively. Linearity for the nitazoxanide and ofloxacin were found in the range of 400-600 μg/ml and 160-240 μg/ml respectively. The method was reproducible, with good resolution between nitazoxanide and ofloxacin and can be use for routine analysis.

Keywords

RP-HPLC, Nitazoxanide and Ofloxacin.

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