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Tiwari, Preeti
- Estimation of Total Phenolics and Flavonoids and Antioxidant Potential of Drakshasava Prepared by Traditional and Modern Methods
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Authors
Affiliations
1 Head of Department of Pharmacognosy and Phytochemistry, IIMT College of Medical Sciences, Meerut (U.P.), IN
2 Head of Department of Pharmacognosy, Shri S. K. Patel College of Pharmaceutical Education and Research, Kherva-382711, Gujarat, IN
1 Head of Department of Pharmacognosy and Phytochemistry, IIMT College of Medical Sciences, Meerut (U.P.), IN
2 Head of Department of Pharmacognosy, Shri S. K. Patel College of Pharmaceutical Education and Research, Kherva-382711, Gujarat, IN
Source
Asian Journal of Research in Chemistry, Vol 6, No 3 (2013), Pagination: 204-208Abstract
The objective of the present study was to estimate the total phenolic content as well as flavonoids in Drakshasava-T and Drakshasava-M prepared by traditional and modern methods respectively and in its marketed preparation and also to evaluate the antioxidant activity of these test preparations on two different in vitro antioxidant activity models. Total phenolic content was determined colorimetrically using Folin-Ciocalteu reagent and was found 0.1045 and 0.1041 %w/w gallic acid equivalent in Drakshasava-T and Drakshasava-M respectively. Total flavonoid content was determined by aluminium chloride method and was found 0.01264 and 0.01214 %w/w quercetin equivalent in Drakshasava-T and Drakshasava-M respectively. Super-oxide anion scavenging activity and lipid per-oxidation assay were carried out to evaluate the antioxidant potential of Drakshasava-T and Drakshasava-M. The antioxidant activity of Drakshasava-T and Drakshasava-M was found increased in concentration dependent manner in both the in vitro antioxidant activity models as super-oxide radical scavenging activity and lipid per-oxidation assay. Drakshasava-T and Drakshasava-M showed significant scavenging of super-oxide radical and showed IC50 120.63 and 128.36 μg/ml respectively. Drakshasava-T and Drakshasava-M also inhibited the ferrous sulphate induced lipid per-oxidation in dose dependent manner and showed inhibitory concentration (IC50) 212.50 and 220.15 μg/ml respectively. Marketed Drakshasava also showed a rich concentration of total phenolics and flavonoids and showed dose dependent antioxidant activity in both the models. Thus, the results obtained in this study indicate that Drakshasava-T and Drakshasva-M can be a promising source of natural antioxidant.Keywords
Total Phenolics, Flavonoids, Antioxidant Potential, Drakshasava
References
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- Development and Validation of HPTLC Method for Quantification of Gallic acid and Catechin from Draksharishta
Abstract Views :315 |
PDF Views:1
Authors
Affiliations
1 Department of Pharmacognosy, Shri Sarvajanik Pharmacy College, Mehsana-384001, Gujarat, IN
2 Department of Pharmaceutical Chemistry, Shri Sarvajanik Pharmacy College, Mehsana-384001, Gujarat, IN
3 Head of Department of Pharmacognosy, Shri S. K. Patel College of Pharmaceutical Education and Research, Kherva-382711, Gujarat, IN
1 Department of Pharmacognosy, Shri Sarvajanik Pharmacy College, Mehsana-384001, Gujarat, IN
2 Department of Pharmaceutical Chemistry, Shri Sarvajanik Pharmacy College, Mehsana-384001, Gujarat, IN
3 Head of Department of Pharmacognosy, Shri S. K. Patel College of Pharmaceutical Education and Research, Kherva-382711, Gujarat, IN
Source
Asian Journal of Research in Chemistry, Vol 6, No 3 (2013), Pagination: 248-253Abstract
A simple, precise and accurate HPTLC method has been established for the determination of gallic acid and catechin in test formulations of Draksharishta as Draksharishta-T and Draksharishta-M prepared by traditional and modern method respectively and also in its marketed formulation. Draksharishta is a polyherbal hydro alcoholic preparation and is used to improve digestion, as blood purifier, in the treatment of anaemia and advised as a choice of remedy in respiratory problems. The developed HPTLC method was validated in terms of precision, accuracy, LOD, LOQ and specificity. The amount of gallic acid in Draksharishta-T, M and in its marketed formulation was found to be 0.0227, 0.0225 and 0.0226 % w/w respectively while catechin was found to be 0.0176, 0.0175 and 0.0175 %w/w respectively. Quantification of gallic acid and catechin in Draksharishta has been reported first time by validated HPTLC. Furthermore, no TLC densitometric methods have been reported for the quantification of gallic acid and catechin from Draksharishta.Keywords
Draksharishta, HPTLC, Validation, Gallic Acid, CatechinReferences
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- Baydar NG, Ozkan G, Sagdic O. Total phenolic contents and antibacterial activities of grape (Vitis vinifera L.) extracts. Food Control 2004; 15: 335-339.
- Frankel EN, Kanner J, German JB, parks E, Kinsella JE. Inhibition of oxidation of human low density lipoprotein by phenolic substances in red wine. Lancet 1993; 341 (20): 454-457.
- Mayer AS, Yi OS, Person DA, waterhouse DL, Frankel EN. Inhibition of human low density lipoprotein oxidation in relation to composition of phenolic antioxidants in grapes (Vitis vonofera). Journal of Agriculture and Food Chemistry 1997; 45: 1638-1643.
- Teissure PL, Frankel EN, Waterhouse AL, Peleg H, German GB. Inhibition of in vitro human LDL oxidation by phenolic antioxidants from grapes and wines. Journal of Science Food and Agriculture 1996; 70:55-61.
- Waterhouse AL. Wine antioxidants may reduce heart disease and cancer. Presentation of American Chemical Society, Washington; 1994.
- Mishra S, Bhaisazya Kalpana Vigyan. Varanasi (India): Chaukambha Surbharati Prakashan; 2005:253-254.
- Alam M, Radhamani S, Ali U, Puroshottam KK. Microbiological screening of dhataki flowers. Journal of research in Ayurveda and Siddha 1984; 2(4): 371-375.
- ICH guidelines Q2 (R1). Validation of analytical procedures. Methodology, Geneva, 2006.
- Singh B, Mungara P, Nivsarkar M, Anandjiwala S. HPTLC densitometry quantification of glycyrrhizin, glycyrrhizinic acid, apigenin, kaempferol and quercetin from Glycyrrhiza glabra. Chromatographia 2009; 70: 1665-1672.
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- Development and Validation of Simultaneous Equation UV-Spectrophotometric Method for the Estimation of Telmisartan and Amlodipine besylate in Combined Dosage Form
Abstract Views :311 |
PDF Views:1
Authors
Affiliations
1 Department of Pharmaceutical Chemistry, IIMT College of Medical Sciences, Meerut-250001
2 HOD, Department of Pharmaceutical Chemistry, IIMT College of Medical Sciences, Meerut
1 Department of Pharmaceutical Chemistry, IIMT College of Medical Sciences, Meerut-250001
2 HOD, Department of Pharmaceutical Chemistry, IIMT College of Medical Sciences, Meerut
Source
Asian Journal of Research in Chemistry, Vol 6, No 8 (2013), Pagination: 765-768Abstract
A simple, sensitive, precise and specific UV spectrophotometric method for simultaneous estimation of Telmisartan and Amlodipine besylate in combine dosage form has been developed. The wavelengths 238 nm and 295 nm were selected for estimation of Amlodipine besylate and Telmisartan respectively, where Methanol was used as solvent. Linearity were observed in the concentration range of 2-25 µg/ml (r2=0.9994) and 2-30 µg/ml (r2=0.9999) for Telmisartan and Amlodipine besylate respectively. These methods were validated according to ICH guidelinesKeywords
Telmisartan, Amlodipine Besylate, Simultaneous Equation MethodReferences
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- Development and Validation of Simultaneous Equation UV-Spectrophotometric Method for the Estimation of Metformin HCl and Glimepiride in Combined Dosage Form
Abstract Views :416 |
PDF Views:2
Authors
Affiliations
1 Department of Pharmaceutical Chemistry, IIMT College of Medical Sciences, Meerut-250001
2 HOD, Department of Pharmaceutical Chemistry, IIMT College of Medical Sciences, Meerut
1 Department of Pharmaceutical Chemistry, IIMT College of Medical Sciences, Meerut-250001
2 HOD, Department of Pharmaceutical Chemistry, IIMT College of Medical Sciences, Meerut
Source
Asian Journal of Research in Chemistry, Vol 6, No 9 (2013), Pagination: 845-848Abstract
A simple, sensitive, precise and specific UV spectrophotometric method for simultaneous estimation of Metformin HCl and Glimepiride in combined dosage form has been developed. Two wavelengths 233 nm and 228.4 nm were selected for estimation of Metformin HCl and Glimepiride respectively. Acetonitrile was used as solvent for analysis of both the drugs. Linearity was observed in the concentration range of 5-10 μg/ml (r2=0.9992) and 3-18 μg/ml (r2=0.9999) for Metformin HCl and Glimepiride respectively. The method was validated as per ICH guidelines.References
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- Development and Validation of Stability Indicating High-Performance Liquid Chromatographic Method for Determination of Pramipexole in Solid Dosage Forms
Abstract Views :213 |
PDF Views:0
Authors
Affiliations
1 Shree Sarvajanik Pharmacy Collage, Near Arvind Baug , Mehsana-384001, Gujarat, IN
1 Shree Sarvajanik Pharmacy Collage, Near Arvind Baug , Mehsana-384001, Gujarat, IN
Source
Asian Journal of Research in Chemistry, Vol 4, No 9 (2011), Pagination: 1393-1397Abstract
The Objective Of the current study was to develop a validated stability indicating high performance liquid chromatographic method for Pramipexole in solid dosage form. The method was validated by subjecting the drugs to forced decomposition under hydrolysis, Oxidation, photolysis, and thermal stress conditions prescribed in international Conference on Harmonization. The drug was successfully separated from major and minor degradation products on a reversed -phase Zorbex SB CN column by using Tri Ethyl Amine buffer (PH 7): Methanol (65:35%V/V) as the mobile phase with determination at 263 nm. The flow rate was 1 ml/min. The method was validated with respect to linearity, precision, accuracy, robustness. The response was linear over the range of 2-24 for Pramipexole. The recovery of the drugs from a mixture product was in the range of 99.60-101.84%. The utility of the procedure was verified by its application to marketed formulations that were subjected to accelerated stability studies.Keywords
Pramipexole, Stability Assay, High Performance Liquid Chromatography, Validation, Mirapex.- Development and Validation of Spectrophotometric Method for Determination of Pramipexole in Solid Dosage Forms
Abstract Views :180 |
PDF Views:0
Authors
Affiliations
1 Shree Sarvajanik Pharmacy Collage, Near Arvind Baug, Mehsana-384001, Gujarat, IN
1 Shree Sarvajanik Pharmacy Collage, Near Arvind Baug, Mehsana-384001, Gujarat, IN
Source
Asian Journal of Research in Chemistry, Vol 4, No 8 (2011), Pagination: 1340-1342Abstract
The Objective Of the current study was to develop and validated UV spectrophotometric method for Pramipexole in solid dosage form. The method was validated by subjecting the drug to UV radiation. The drug was successfully quantified at 263 nm. The method was validated with respect to linearity, precision, accuracy, robustness. The response was linear over the range of 3-15 for Pramipexole. The recovery of the drugs from a mixture product was in the range of 99.00-101.46%. The utility of the procedure was verified by its application to marketed formulations. The specificity of the method was also checked with reference to placebo. The method was found to be specific also, hence other validation parameter were in the limit also.Keywords
Pramipexole, Assay, UV Spectrophotometric, Validation, Mirapex.- Quantification of Quercetin and Rutin in Arjunarishta Prepared by Traditional and Modern Methods by Validated HPTLC Densitometry
Abstract Views :145 |
PDF Views:0
Authors
Affiliations
1 Department of Pharmacognosy, Shri Sarvajanik Pharmacy College, Mehsana-384001, IN
2 Department of Pharmacognosy, Shri S. K. Patel College of Pharmaceutical Education and Research, Kherva-382711, IN
1 Department of Pharmacognosy, Shri Sarvajanik Pharmacy College, Mehsana-384001, IN
2 Department of Pharmacognosy, Shri S. K. Patel College of Pharmaceutical Education and Research, Kherva-382711, IN