Asian Journal of Research in Chemistry

Prasanna Reddy Battu ¹, M. S. Reddy ²

Affiliations
1 Dept. Quality Control and Analytical R and D, Smilax Laboratories Limited, Jeedimetla, Hyderabad-500055, A.P, IN
2 Department of Entomology and Plant Pathology, Auburn University, US

Abstract

A simple, selective, accurate high Performance Liquid Chromatographic (HPLC) method was developed and validated for the analysis of Rebeprazole sodium. Chromatographic separation achieved isocratically on a C₁₈ column [Use Inertsil C₁₈, 5 μ, 150 mm×4.6 mm] utilizing a mobile phase of acetonitrile/phosphate buffer (70:30, v/v, pH 7.0) at a flow rate of 0.8 ml/min with UV detection at 228 nm. The retention time of Rabeprazole sodium was 2.44 min. The method is accurate (99.15-101.85%), precise (intra-day variation 0.13-1.56% and inter-day variation 0.30-1.60%) and linear within range 0.1-30 μg/ml (R²=0.999) concentration and was successfully used in monitoring left over drug. The detection limit of Rabeprazole sodium at a signal-to-noise ratio of 3 was 1.80 ng/ml in human plasma while quantification limit in human serum was 5.60 ng/ml. The proposed method is applicable to routine analysis of Rabeprazole sodium in pharmaceutical formulations as well as in human plasma samples.

Keywords

Rabeprazole Sodium, RP-HPLC, Validation, Human Blood Samples, Pharmaceutical Dosage Forms.

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Prasanna Reddy Battu ¹, M. S. Reddy ²

Affiliations
1 Dept. Quality Control and Analytical R and D, Smilax Laboratories Limited, Jeedimetla, Hyderabad-500055, A.P, IN
2 Department of Entomology and Plant Pathology, Auburn University, US

Abstract

A simple, selective, accurate high Performance Liquid Chromatographic (HPLC) method was developed and validated for the analysis of Paracetamol and Ibuprofen. Chromatographic separation achieved isocratically on a C₁₈ column [Use Inertsil C₁₈, 5 μ, 150 mm×4.6 mm] utilizing a mobile phase of acetonitrile/phosphate buffer (60:40, v/v, pH 7.0) at a flow rate of 0.8 ml/min with UV detection at 260 nm. Aceclofenac was used as an internal standard. The retention time of ibuprofen, paracetamol and aceclofenac was 2.48, 4.45 and 6.34 min respectively. The developed method was validated in terms of accuracy, precision, linearity, limit of detection, limit of quantitation. This study aimed at developing and validating an HPLC method, being simple, accurate and selective, and the proposed method can be used for the estimation of these drugs in combined dosage forms.

Keywords

Paracetamol, Ibuprofen, RP-HPLC, Validation.

Full Text

     

Prasanna Reddy Battu ¹, M. S. Reddy ²

Affiliations
1 Dept. Quality Control and Analytical R and D, Smilax Laboratories Limited, Jeedimetla, Hyderabad-500055, A.P, IN
2 Department of Entomology and Plant Pathology, Auburn University, US

Abstract

Twenty Pseudomonas fluorescens strains were isolated from rice growing soil samples and characterized. One of the P.fluorescens isolated and identified from the dual culture test. It was fermented for secondary metabolite in a small scale and extracted with ethyl acetate. The isolated metabolite tested against rice fungal pathogens. The structure of the compound was elucidated by high-resolution NMR spectroscopy.

Keywords

Pseudomonas fluorescens, Antimicrobial Metabolites, Rice Fungal Pathogens.

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